Low‐level Laser Therapy Ameliorates CCl₄‐induced Liver Cirrhosis in Rats

Abstract

This study investigated the effects of low-level laser therapy (LLLT) in the liver function, structure and inflammation in a experimental model of carbon tetrachloride (CCl(4))-induced liver cirrhosis. Wistar rats were divided into Control, LLLT, CCl(4) and CCl(4) +LLLT groups. CCl(4) groups received CCl(4) (0.4 g kg(-1); i.p.), three times a week, for 12 weeks. A 830 nm LLLT was performed with a continuous wave, 35 mW, 2.5 J cm(-2) per point, applied to four points of the liver (right and left upper and lower extremities, in the four lobes of the liver) for 2 weeks. Liver structure and inflammation (cirrhotic areas, collagen deposition, inflammation, density of Kupffer and hepatic stellate cells) and function (aspartate aminotransferase, alkaline phosphatase, gamma glutamyltransferase, lactate dehydrogenase, total proteins and globulins) were evaluated. LLLT significantly reduced CCl(4)-increased aspartate aminotransferase (P < 0.001), alkaline phosphatase (P < 0.001), gamma-glutamyl transferase (P < 0.001) and lactate dehydrogenase (P < 0.01) activity, as well as total proteins (P < 0.05) and globulins (P < 0.01). LLLT also reduced the number of cirrhotic areas, the collagen accumulation and the hepatic inflammatory infiltrate. Of note, LLLT reduced CCl(4)-increased number of Kupffer cells (P < 0.05) and hepatic stellate cells (P < 0.05). We conclude that LLLT presents beneficial effects on liver function and structure in an experimental model of CCl(4)-induced cirrhosis.

Photobiomodulation therapy inhibits oral submucous fibrosis in mice

“…Sassoli et al (2016) showed that PBM inhibited the TGF-β1/Smad3mediated fibroblast-myofibroblast transition. Additionally, PBM has been reported to reduce collagen deposition in rat tendons after trauma (Fillipin et al, 2005), carbon tetrachloride-induced liver cirrhosis (Oliveira-Junior et al, 2013), and renal interstitial fibrosis (Oliveira et al, 2012). According to our previous in vitro study, we showed that PBM could significantly reduce the pathological thickness in ANE-induced OSMF.…”

Melatonin application in targeting oxidative‐induced liver injuries: A review

“…CCl 4 has been used as the most common experimental model of chemically induced liver fibrosis (Ogeturk et al, ) and cirrhosis (Oliveira‐Junior et al, ) in rodents causing damages resemble those seen in human subjects (Mortezaee, Sabbaghziarani, et al, ). Metabolization of CCl 4 produces • CCl 3 that reacts with O 2 to form CCl 3 (Shaker et al, ).…”

“…CCl 4 has been used as the most common experimental model of chemically induced liver fibrosis (Ogeturk et al, 2008) and cirrhosis (Oliveira-Junior et al, 2013) in rodents causing damages resemble those seen in human subjects .…”

Effects of pre-incubation laser irradiation on hatchability and small intestine enzymes activity in post-hatched broiler chickens

“…Alkaline phosphatase (ALP) is another prominent enzyme from the group of catalytic enzymes, frequently distributed in brush border of intestinal mucosal cytosol, degrading phosphate esters and separating phosphoric acid molecules (Ghiasi Ghalehkandi et al, 2012). It is reported that low-level laser therapy significantly diminished carbon tetrachloride-increased ALP of rat liver (Oliveira-Junior et al, 2013).…”