Chalcones from Myracrodruon urundeuva are efficacious in guinea pig ovalbumin-induced allergic conjunctivitis

Abstract

Abstract:The objective of the study was to verify the effects of dimeric chalcones (urundeuvines A, B, and C) from Myracrodruon urundeuva Allemão, Anacardiaceae (a Brazilian anti-inflammatory species), on an allergic conjunctivitis model. Male guinea-pigs were sensitized with two intraperitoneal injections of ovalbumin (10 μg dissolved in 0.5 mL saline and emulsified in 0.5 mL Freund's adjuvant), at days 0 and 7. At day 24, the animals were submitted to an ocular instillation (right eyes) with ovalbumin. At the next day, the animals were treated with chalcones (0.5 mg, three times a day for 7 days), 0.1% fluormetalone acetate (0.05 mg, as the reference drug) or saline. After anesthesia of the animals, enucleations of their corneas and conjunctivas were carried out for morphometric and histological analyses, at days 1, 3 and 7. Their radical scavenging activity and action on myeloperoxidase were also determined. We demonstrated that chalcones from M. urundeuva stem barks presented anti-inflammatory and antioxidant effects, and drastically inhibited the MPO activity, pointing them as candidates for the treatment of allergic conjunctivitis and other inflammatory conditions.

Gastroprotective and anti-inflammatory activities integrated to chemical composition of Myracrodruon urundeuva Allemão - A conservationist proposal for the species

“…In the shoot stems, the presence of urundeuvine A is observed, as described by Bandeira (2002), as well as a derivative of urundeuvine A. Chalcones represent a class of compounds with pronounced gastroprotective activity (Mota et al, 2009). Urundeuvines isolated from M. urundeuva have a pronounced antiulcerogenic effect, in addition to the anti-inflammatory, analgesic (Viana et al, 2003;Albuquerque et al, 2011;Rodrigues et al, 2002) and also neuroprotective effects (Calou et al, 2014;Nobre-Júnior et al, 2009). In the shoot leaves, the presence of gallotannins, such as tri-galloyl hexoside, tetragalloylglucose, penta galloyl-hexoside and hexagalloylhexoside was observed in the present study.…”

Assessment of Phenolic Compounds and Anti-Inflammatory Activity of Ethyl Acetate Phase of Anacardium occidentale L. Bark

“…Bernardes et al [62], using S. terebinthifolius extract, showed a reduction in the inflammatory process exacerbated in their studies and found antimycobacterial activity attributed to the flavonoids identified in this extract. Studies of the Myracrodruon urundeuva , also known in ethnopharmacology for its anti-inflammatory action, confirmed the presence of tannins and flavonoids in various parts of the plant [63,64].…”

Evaluation of the Healing Potential of Myracrodruon urundeuva in Wounds Induced in Male Rats

“…Jandú et al (2013), Gomes et al (2013), and Sá et al (2009) demonstrated the antibacterial activity of "aroeira" against gram-positive and gram-negative bacteria, mainly by the composition of their secondary metabolites that mainly involve tannins, flavonoids, and alkaloids. Others (Albuquerque et al 2011) demonstrated that chalcones from M. urundeuva stem barks presented anti-inflammatory and antioxidant effects and drastically inhibited myeloperoxidase (inflammation marker) activity pointing them as potential candidates for the treatment of inflammatory conditions. In addition, an important neuroprotective property of a standardized extract (rich in tannins and chalcones) from M. urundeuva that was shown to be effective on a Parkinson's disease model in rats (Calou et al 2014).…”