Gastroprotective and anti-inflammatory activities integrated to chemical composition of Myracrodruon urundeuva Allemão - A conservationist proposal for the species

Galvão, Wagner Rodrigues; Braz-Filho, Raimundo; Canuto, Kirley Marques; Ribeiro, Paulo Riceli Vasconcelos; Campos, Adriana Rolim; Moreira, Ana Cristina de Oliveira Monteiro; Silva, S.O.; Filho, F.A. Mesquita; S.A.A.R., Santos; Melo, Jeane Martins; Gonçalves, Nidyedja Goyanna Gomes; Fonseca, Said Gonçalves da Cruz; Bandeira, M. A. M.

doi:10.1016/j.jep.2018.04.024

Cited by 24 publications

(12 citation statements)

References 40 publications

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“…Portulaca olearacea [40] and Protium spruceanum [41], both rich in quercitrin, proved to suppress NF-kB/activate MAPK and exerted immunomodulation/enzyme activation, which could lead to their anti-inflammatory effect. Finally, the anti-inflammatory action of Myracrodruon urundeuva and Tetraclinis articulata is correlated to the presence of afzelin [42,43]. The presence of these three compounds as the main constituents of Crataegus almaatensis explains in part the anti-inflammatory action obtained for APCa.…”

Section: Discussionmentioning

confidence: 99%

Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian Herbal Medicine

et al. 2019

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Crataegus almaatensis, an endemic ornamental plant in Kazakhstan is used in popular medicine due to its cardiotonic properties. The most studied species of the same genus are commonly found in Europe, which shows the importance of having the Kazakh species validated via its chemical and pharmacological studies. High-speed countercurrent chromatography (HSCCC) operated under optimized conditions enabled an isolation of the three main compounds from the aqueous phase of the leaves ethanol extract, further identified by nuclear magnetic resonance (NMR), as quercetin 3-O-rhamnoside (quercitrin) (4.02% of the crude extract-CECa); quercetin 3-O-β-galactoside (hyperoside) (1.82% of CECa); kaempferol 3-O-α-L-rhamnoside (afzelin) (0.94% of CECa). The CECa, the aqueous phase of the crude extract (APCa) together with the isolates were evaluated for their vascular (vascular reactivity in human internal mammary artery-HIMA), anti-nociceptive (formalin-induced liking response and hot plate) and anti-inflammatory (subcutaneous air-pouch model-SAP) activities. CECa at the concentrations of 0.014 and 0.14 mg/mL significantly increased the maximum contractility response of HIMA to noradrenaline. The APCa CR curve (0.007–0.7 mg/mL) showed an intrinsic relaxation effect of the HIMA. APCa at the dose of 100 mg/kg i.p. significantly decreased the total leukocyte count and the IL-1β release in the SAP wash.

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Section: Discussionmentioning

confidence: 99%

Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian Herbal Medicine

et al. 2019

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“…Solid phase extraction (SPE) was performed according to Aguiar-Galvão et al ( 22 ), using a Supelclean (C18, 500 mg) reverse phase cartridges. Briefly, 20 mg of sample were dissolved in 200 μL of methanol (MeOH).…”

Section: Methodsmentioning

confidence: 99%

“…The fractions were analyzed in the positive (PI) and negative (NI) ionization modes in a range of 100-1,200 Da. ESI conditions were defined as follows: capillary voltage 2800 V, cone voltage 40 V, source temperature 120°C, dissolution temperature 330°C, cone gas flow of 20 L/h, gas desolvation flow 600 L/h, and MCP (microchannel plate voltage)-detector at 1,900 V. The compound identification was based on the molecular formula deduced from the exact mass (4 decimal places), considering a mass error lower than 5 parts per million (ppm), the isotopic ion pattern (i-fit) and the ion fragmentation pattern compared to literature data ( 22 ).…”

Section: Methodsmentioning

confidence: 99%

Endophytic Fungus Isolated From Achyrocline satureioides Exhibits Selective Antiglioma Activity—The Role of Sch-642305

et al. 2018

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Glioblastoma is the most devastating primary brain tumor. Current treatment is palliative, making necessary the development of new therapeutic strategies to offer alternatives to patients. Therefore, endophytes represent an interesting source of natural metabolites with anticancer potential. These microorganisms reside in tissues of living plants and act to improve their growth. Evidence revealed that several medicinal plants are colonized by endophytic fungi producer of antitumor metabolites. Achyrocline satureioides is a Brazilian medicinal plant characterized by its properties against gastrointestinal disturbances, anticancer and antioxidant effects. However, there are no reports describing the endophytic composition of A. satureioides. The present study proposes the isolation of endophytic fungus from A. satureioides, extract preparation, phytochemical characterization and evaluation of its antiglioma potential. Our data showed that crude extracts of endophyte decreased glioma viability with IC50 values of 1.60–1.63 μg/mL to eDCM (dichloromethane extract) and 37.30–55.12 μg/mL to eEtAc (ethyl acetate extract), respectively. Crude extracts induced cell death by apoptosis with modulation of redox status. In order to bioprospect anticancer metabolites, endophytic fungus extracts were subjected to guided fractionation and purification yielded five fractions of each extract. Six of ten fractions showed selective antiproliferative activity against glioma cells, with IC50 values ranged from 0.95 to 131.3 μg/mL. F3DCM (from eDCM) and F3EtAc (from eEtAc) fractions promoted C6 glioma toxicity with IC50 of 1.0 and 27.05 μg/mL, respectively. F3EtAc fraction induced late apoptosis and arrest in G2/M stage, while F3DCM promoted apoptosis with arrest in Sub-G1 phase. Moreover, F3DCM increased antioxidant defense and decreased ROS production. Additionally, F3DCM showed no cytotoxic activity against astrocytes, revealing selective effect. Based on promising potential of F3DCM, we identified the production of Sch-642305, a lactone, which showed antiproliferative properties with IC50 values of 1.1 and 7.6 μg/mL to C6 and U138MG gliomas, respectively. Sch-642305 promoted arrest on cell cycle in G2/M inducing apoptosis. Furthermore, this lactone decreased glioma cell migration and modulated redox status, increasing superoxide dismutase and catalase activities and enhancing sulfhydryl content, consequently suppressing reactive species of oxygen generation. Taken together, these results indicate that metabolites produced by endophytic fungus isolated from A. satureioides have therapeutic potential as antiglioma agent.

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“…É de conhecimento popular local a ação analgésico, anti-inflamatório, antioxidante, antifúngico e antibacteriano da aroeira. Na literatura há relatos que tais benefícios a saúde estão relacionados principalmente aos compostos bioativos: polifenóis, taninos, flavonoides e alcaloides AGUIAR GALVÃO et al, 2018). Pesquisas recentes lingam os bioativos de aroeira a propriedades de prevenção ou tratamento de distúrbios neurodegenerativos, doenças que são crescentes na população mundial (VIANA; CALOU, 2019).…”

Section: Introductionunclassified

“…Resultados com efeito analgésico, anti-inflamatório, antioxidante, antifúngico e antibacteriano de várias partes da arvore, também são de amplo conhecimento popular local e estão associados a compostos bioativos, como os polifenóis, taninos, flavonoides e alcaloides AGUIAR GALVÃO et al, 2018). Pesquisas recentes relacionaram os bioativos de aroeira a prevenção ou tratamento de distúrbios neurodegenerativos (VIANA; CALOU, 2019).…”

Section: Introductionunclassified

Conservação in vitro da aroeira (myracrodruon urundeuva fr. Allemão) e estabelecimento in vitro da baraúna (schinopsis brasiliensis engl.)

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Gastroprotective and anti-inflammatory activities integrated to chemical composition of Myracrodruon urundeuva Allemão - A conservationist proposal for the species

Cited by 24 publications

References 40 publications

Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian Herbal Medicine

Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian Herbal Medicine

Endophytic Fungus Isolated From Achyrocline satureioides Exhibits Selective Antiglioma Activity—The Role of Sch-642305

Conservação in vitro da aroeira (myracrodruon urundeuva fr. Allemão) e estabelecimento in vitro da baraúna (schinopsis brasiliensis engl.)

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