Behavioral and enzymatic bioassays with Serjania erecta Radlk., Sapindaceae, correlated with cognitive dysfunctions

Abstract

RESUMO: "Comportamento e ensaios bioenzimáticos com Serjania erecta Radlk., sapindaceae, correlacionados com disfunções cognitivas." O objetivo deste estudo foi pesquisar o extrato bruto de Serjania erecta Radlk., Sapindaceae, e seus bioativos como preventivos ou inibidores de perda de memória em roedores, e outros fatores correlacionados com a síndrome de Alzheimer: atividade antioxidante e anticolinesterásicas, principalmente como planta adaptógena, baixa toxicidade e ação regulatória. A reversão do bloqueador colinérgico (escopolamina) no teste da esquiva passiva foi detectada pela latência mensurada em animais jovens e adultos. Apresentou baixa toxicidade, com efeito protetor na análise bioquímica (hipoglicemia/hipotrigliceridemia). Índices elevados (acima 83%) na atividade antioxidante foram observados. A inibição da AChE e BuChE foi perceptível nas frações cromatográficas, confirmando as ações via oral e diretamente no SNC.Unitermos: Serjania erecta, antioxidante, anticolinesterase, adaptógena, Alzheimer. ABSTRACT:The purpose of this study was to investigate the crude extract of Serjania erecta Radlk., Sapindaceae, and its bioactive agents as preventive or inhibitor of memory loss in rodents, as well as other factors correlated with Alzheimer's syndrome: antioxidant and anticholinesterase activity, mainly as plant adaptogen -low toxicity and regulation action. The blocking cholinergic reversion activity (scopolamine) in the test of the passive avoidance was detected by measuring latency in young and adult animals. It presented low toxicity, with protective effect as shown by biochemical analysis (hypoglycemic/ hypotriglyceridemic). Elevated levels (above 83%) of antioxidant activity were detected. AchE and BuChE inhibition were also detected in the chromatographic fractions, which were active both orally and directly on CNS (ICV).

Inhibitory effects of Serjania erecta on the development of Chrysodeixis includens

“…The delayed development and weight loss observed in C. includens may have been related to the phenolic compounds found in the S. erecta extract, including kaempferol 28 , saponins 29 , and apigenins 28 . Phenolic compounds may cause a significant reduction in the survival and growth of insects due to the inactivation of digestive enzymes 30 .…”

“…Regarding S. erecta , there are few reports in the literature regarding the inhibitory activity of its extracts. Broggini et al 29 showed the inhibitory effect of crude extracts of this species using thin layer chromatography. In this work, the flavonoid fraction of this extract was shown to be more selective for inhibition of acetylcholinesterase than for that of butyrylcholinesterase, while the saponin fraction showed greater inhibition of butyrylcholinesterase than of acetylcholinesterase 29 .…”

“…Broggini et al 29 showed the inhibitory effect of crude extracts of this species using thin layer chromatography. In this work, the flavonoid fraction of this extract was shown to be more selective for inhibition of acetylcholinesterase than for that of butyrylcholinesterase, while the saponin fraction showed greater inhibition of butyrylcholinesterase than of acetylcholinesterase 29 . Thus, the presence of metabolites in the extract of this plant, at a concentration of 31.3 µg mL −1 , is high enough to totally inhibit the activity of carboxyl-esterases.…”

Toxicological, biochemical and morphophysiological effects of Serjania erecta leaf aqueous extract on Piaractus mesopotamicus

“…The aqueous extract was obtained by the method described by Broggini et al (2010), with adaptations. Leaves were dried in a forced-air circulation incubator with microprocessor and air renewal (Sterilifer -SXCR 40) at 37 ± 2 °C for 48 h and ground through a Wiley mill (TE-680-Tecnal) with a 10-mesh screen.…”

“…[e.g. Serjania marginata (Périco et al 2015, Moreira et al 2019, Serjania caracasana (Silva et al 2017), and S. erecta (Castelo et al 2009, Broggini et al 2010] in mice. However, no studies have evaluated the toxicity of S. erecta in aquatic organisms.…”