Toxicological, biochemical and morphophysiological effects of Serjania erecta leaf aqueous extract on Piaractus mesopotamicus

Ventura, Arlene Sobrinho; Filho, Ruy Alberto Caetano Corrêa; Spica, Louise Nex; Silva, Ana Cremilda F.; Araújo, Andréa Maria de; Cardoso, Cláudia Andréa Lima; Jerônimo, Gabriela Tomas; Povh, Jayme Aparecido

doi:10.1590/0001-3765202120190479

Cited by 2 publications

(1 citation statement)

References 35 publications

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“…The biological effects attributed to S. erecta in traditional and indigenous medicine may be related to the bioactive compounds found in this species. Qualitative analyses have already identified the presence of saponins, flavonoids, triterpenoids, steroids, tannins, and catechins in the hydroalcoholic extract of the leaves ( Gomig et al, 2008 ; Ventura et al, 2021 ). Similarly, analyses of the crude extract using nuclear magnetic resonance showed the presence of flavonoids such as camferol, epicatechins, apigenins, vitexin, isovitexin, and quercetin ( Guimarães et al, 2015 ; Cardoso et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

The morphoanatomy of Serjania erecta Radlk (Sapindaceae) provides evidence of biotrophic interactions by endophytic fungi within leaves

Freitas,

Faria,

Silva

et al. 2023

PeerJ

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Background The leaves of Serjania erecta Radlk (Sapindaceae) are renowned in ethnobotany for their medicinal properties and are significant as a medicinal resource for traditional Brazilian communities. As necrotic spots are common on these leaves, indicating interaction with phytopathogenic fungi, it was hypothesized that biotrophic fungal species colonize the leaf tissues of S. erecta. Methods To test this hypothesis, we employed standard techniques in plant anatomy, which enabled us to investigate the interaction of fungal structures with plant tissues and describe the morphoanatomical and histochemical characteristics of the epidermis and limbus of S. erecta. Results The anatomical analysis showed the existence of leaf teeth on the leaf tips. Additionally, hyphae, conidiospores, and spores of Bipolaris/Curvularia species were detected on the adaxial epidermis. Moreover, melanized microsclerotia were found in glandular areas of the leaf teeth and the phloem, providing evidence of biotrophic behavior. The hypothesis that biotrophic phytopathogenic fungi interact with S. erecta leaf tissues was confirmed, despite the presence of many bioactive compounds (such as flavonoids, alkaloids, and essential oils), as evidenced by histochemical analyses. The presence of tector, glandular, and scabiform trichomes on the leaf teeth and epidermis was also revealed. This study presents, for the first time, the synthesis of essential oils and alkaloids in the leaves of S. erecta. Additionally, it investigates previously unexplained aspects of the anatomy and histochemistry of the species, as well as its interaction with resident microorganisms. Therefore, it is recommended that future research focus on extracting and characterizing the oils and alkaloids of S. erecta, as well as exploring other aspects related to its microbiome and its relationship.

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Section: Introductionmentioning

confidence: 99%

The morphoanatomy of Serjania erecta Radlk (Sapindaceae) provides evidence of biotrophic interactions by endophytic fungi within leaves

Freitas,

Faria,

Silva

et al. 2023

PeerJ

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Low microbial diversity, yeast prevalence, and nematode-trapping fungal presence in fungal colonization and leaf microbiome of Serjania erecta

de Freitas,

Silva,

Bessa

et al. 2024

Sci Rep

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Medicinal plant microbiomes undergo selection due to secondary metabolite presence. Resident endophytic/epiphytic microorganisms directly influence plant’s bioactive compound synthesis. Hypothesizing low microbial diversity in Serjania erecta leaves, we assessed leaf colonization by epiphytic and endophytic fungi. Given its traditional medicinal importance, we estimated diversity in the endophytic fungal microbiome. Analyses included scanning electron microscopy (SEM), isolation of cultivable species, and metagenomics. Epiphytic fungi interacted with S. erecta leaf tissues, horizontally transmitted via stomata/trichome bases, expressing traits for nematode trapping. Cultivable endophytic fungi, known for phytopathogenic habits, didn’t induce dysbiosis symptoms. This study confirms low leaf microbiome diversity in S. erecta, with a tendency towards more fungal species, likely due to antibacterial secondary metabolite selection. The classification of Halicephalobus sp. sequence corroborated the presence of nematode eggs on the epidermal surface of S. erecta by SEM. In addition, we confirmed the presence of methanogenic archaea and a considerable number of methanotrophs of the genus Methylobacterium. The metagenomic study of endophytic fungi highlighted plant growth-promoting yeasts, mainly Malassezia, Leucosporidium, Meyerozyma, and Hannaella. Studying endophytic fungi and S. erecta microbiomes can elucidate their impact on beneficial bioactive compound production, on the other hand, it is possible that the bioactive compounds produced by this plant can recruit specific microorganisms, impacting the biological system.

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Toxicological, biochemical and morphophysiological effects of Serjania erecta leaf aqueous extract on Piaractus mesopotamicus

Cited by 2 publications

References 35 publications

The morphoanatomy of Serjania erecta Radlk (Sapindaceae) provides evidence of biotrophic interactions by endophytic fungi within leaves

The morphoanatomy of Serjania erecta Radlk (Sapindaceae) provides evidence of biotrophic interactions by endophytic fungi within leaves

Low microbial diversity, yeast prevalence, and nematode-trapping fungal presence in fungal colonization and leaf microbiome of Serjania erecta

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