The glycosylated flavonoids vitexin, isovitexin, and quercetrin isolated from Serjania erecta Radlk (Sapindaceae) leaves protect PC12 cells against amyloid-β 25-35 peptide-induced toxicity

Protection of Differentiating Neuronal Cells from Amyloid β Peptide-induced Injury by Alkaline Extract of Leaves of Sasa senanensis Rehder

“…Cells were treated for 4,8,12,48,72 stress (10), alteration of metal ion influx (11), endoplasmic reticulum stress (12) and impaired autophagy (13). In order to test the generality of cell protective activity of SE, we investigated here whether SE protects the neuronal cell death induced by amyloid β-peptides (Aβ and Aβ [25][26][27][28][29][30][31][32][33][34][35] ).…”

“…The following chemicals and reagents were obtained from the indicated companies: Dulbecco's modified Eagle's medium (DMEM), human recombinant NGF, dimethyl sulfoxide (DMSO) (Wako Pure Chemical Ind., Ltd., Osaka, Japan); fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich Inc., St. Louis, MO, USA); Aβ and Aβ [25][26][27][28][29][30][31][32][33][34][35] (Cosmo Bio Co., Ltd., Tokyo, Japan). NGF (dissolved in water at 0.5 mg/ml), Aβ (dissolved in DMSO at 1 mM) and Aβ [25][26][27][28][29][30][31][32][33][34][35] (dissolved in water at 0.1 mg/m) were frozen at -20˚C.…”

“…NGF (dissolved in water at 0.5 mg/ml), Aβ (dissolved in DMSO at 1 mM) and Aβ [25][26][27][28][29][30][31][32][33][34][35] (dissolved in water at 0.1 mg/m) were frozen at -20˚C. The following 96-microwell plates were purchased from the indicated companies: TrueLine (Nippon Genetics Co., Ltd., Tokyo, Japan), TPP (Techno Plastic Products AG, Trasadingen, Swizeland), BioCoat™ Collagen I 96-well plate, BioCoat™ Collagen I 96-well plate, BioCoat™ Poly-LLysine 96-well plate (Corning Inc., Corning, Canton, NY, USA).…”

“…Effect of preincubation of Aβ peptides on the cytotoxicity induction. Aβ and Aβ [25][26][27][28][29][30][31][32][33][34][35] solution were preincubated at 37˚C for 0 (control),measure the absorbance at 560 nm with a plate leader (Infinite F 50 R, TECAN, Kawasaki, Japan).…”

“…Undifferentiated (PC12, SH-SY5Y) and differentiated PC12 cells were treated with Aβ 1-42 and Aβ [25][26][27][28][29][30][31][32][33][34][35] for the 24~48 hours in the presence of increasing concentrations of test samples. The viable cell number was determined as described above.…”