Atividade antioxidante de Jacaranda decurrens Cham., Bignoniaceae

Abstract

RESUMO:Muitas doenças e processos degenerativos estão associados à superprodução de espécies reativas de oxigênio (ERO) o que tem estimulado vários grupos de pesquisa a investigarem o potencial antioxidante de substâncias produzidas por diversas famílias da flora mundial. O presente trabalho teve como objetivo avaliar a atividade antioxidante de extrato bruto hidroalcoólico de folhas de Jacaranda decurrrens e de frações obtidas a partir desse extrato, pelo método espectrométrico de descoloração do radical 2,2-difenil-1-picrilhidrazila (DPPH). A atividade antioxidante (91%) do extrato bruto e da fração Jd-2 foi semelhante à atividade obtida com o padrão rutina, na concentração de 1 mg/L (92,56%). A fração Jd-3 quando comparada com as outras frações apresentou maior atividade antioxidante em concentrações igual ou abaixo de 2,5 mg/L. A atividade antioxidante das amostras está relacionada à presença dos triterpenos ácidos ursólico e oleanólico presentes no extrato bruto e nas frações Jd-1 e Jd-2 e também a flavonóides glicosilados contidos na fração Jd-3. Este é o primeiro trabalho demonstrando a atividade antioxidante de extrato de folhas de Jacaranda decurrens.Unitermos: Jacaranda decurrens, Bignoniaceae, planta medicinal, cerrado, antioxidante.ABSTRACT: "Antioxidant activity of Jacaranda decurrens Cham., Bignoniaceae". Since many diseases and degenerative processes have been associated to the overproduction of reactive oxygen species (ROS) many research groups are motivated to investigate the antioxidant potential of substances produced by several families of the world flora. The aim of this work was to evaluate the antioxidant activity of crude hydroalcoholic extract of Jacaranda decurrrens leaves and fractions obtained from that extract, using the free radical DPPH (2,2-difenyl-1-picrylhydrazyl) through spectrometric discoloration method. Results confirmed that either the crude extract or fractions of the extract presented antioxidant activity. The antioxidant activity (91%) demonstrated by the crude extract was equivalent to the antioxidant activity determined for fraction Jd-2 in the concentration of 10 mg/L, and similar to the activity showed by the standard rutin, in the concentration of 1 mg/L (92,56%). Fraction Jd-1 in the concentration of 10 mg/L and 5 mg showed 84% and 86% antioxidant activity, respectively. Those values are significantly different and inferior if compared to the standard rutin. Compared to the other fractions the Jd-3 presented higher antioxidant activity. The antioxidant potential of Jacaranda decurrens crude extracts and fractions Jd-1 and Jd-2 is probably related to the production of the triterpenes ursolic and oleanolic acids and also to the glycosylated flavonoids produced in the fraction Jd-3. This is the first report on the antioxidant activity of crude hydroalcoholic extract of J. decurrrens leaves and fractions obtained from that extract.

Subacute and Reproductive Oral Toxicity Assessment of the Hydroethanolic Extract ofJacaranda decurrensRoots in Adult Male Rats

“…In other study, analyses performed using HPLC indicated the presence of ursolic acid in fractions of the J. decurrens extract [7]. In the present study, the presence of flavonoids luteolin in ethyl acetate fraction and myricetrin and quercetrin in water fraction of the extract was identified (Figure 1).…”

Anti-inflammatory effects and acute toxicity of hydroethanolic extract of Jacaranda decurrens roots in adult male rats

“…Analyses performed using high performance liquid chromatography (HPLC) indicated the presence of ursolic acid in fractions of the Jacaranda decurrens extract (Carvalho et al, 2009), a compound whose molecule is similar to dexamethasone. In the present study, the oleanolic and ursolic acids were isolated of the EJD roots.…”

“…Pharmacological evaluations have revealed that species from Jacaranda genus possess antioxidants (Carvalho et al, 2009), antimicrobials (Zatta et al, 2009), chemopreventives properties (Subbaramaiah et al, 2000). It is suggested that the responsible for these activities are compounds such as ursolic acid.…”

“…It is suggested that the responsible for these activities are compounds such as ursolic acid. Phytochemical analyses of the leaves of Jacaranda decurrens indicated the presence of triterpenes, ursolic acid, oleanolic, flavonoid, saponins and coumarins (Carvalho et al, 2009;Zatta et al, 2009).…”

Produtividade de vinagreira, pimenta rosa e carobinha cultivadas em sucessão a mucuna preta e feijão de porco

“…symmetrifoliolata (Bignoniaceae, carobinha). Esse subarbusto endêmico do Cerrado (Farias & Proença, 2003) encontra-se em risco de extinção, devido principalmente à expansão do uso da terra (Bertoni et al, 2010) e pelas propriedades medicinais das suas raízes como antioxidante (Carvalho et al, 2009), antibacteriana (Zatta et al, 2009) e antifúngica (Fenner et al, 2006).…”