Produção de metabólitos secundários em cultura de células e tecidos de plantas: o exemplo dos gêneros Tabernaemontana e Aspidosperma

Fumagali, Elisângela; Gonçalves, Regina Aparecida Correia; Machado, Maria de Fátima Pires da Silva; Vidoti, Gentil José; Oliveira, Arildo José Braz de

doi:10.1590/s0102-695x2008000400022

Cited by 58 publications

(42 citation statements)

References 62 publications

(56 reference statements)

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“…Biotechnological alternatives, such as the use of endophytic microorganisms, culture of cells and tissues, micropropagation, and genetic improvement by crossing can be employed to increase the production of secondary metabolites in plants. Another sustainable technology that has favored an increase in compounds of secondary metabolism is the use of arbuscular mycorrhizal fungi (AMF) …”

Section: Introductionmentioning

confidence: 99%

Production of biomolecules of interest to the anxiolytic herbal medicine industry in yellow passionfruit leaves (Passiflora edulis f. flavicarpa) promoted by mycorrhizal inoculation

et al. 2019

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BACKGROUND Our contemporary way of life has led us to consume high amounts of chemically‐synthesized allopathic medicinal products and anxiolytics to which a viable alternative is the use of Passiflora‐based herbal medicines with composition containing vitexin, a flavonoid with anxiolytic and antidepressant properties. Arbuscular mycorrhizal fungi (AMF) are known for enhancing the production of biomolecules, however, increase production of phytochemistry in Passiflora edulis f. flavicarpa has not been reported in the literature. Our aim was to select AMF to benefit the production of vitexin in leaves of P. edulis by inoculating seedlings in the region of roots with Acaulospora longula, Claroideoglomus etunicatum and Gigaspora albida. RESULTS The inoculation increased the concentration of vitexin in 63.64% and the inoculation with A. longula also increased the content of flavonoids and total saponins in the leaves in relation to the control. CONCLUSION The increase in the production of vitexin in the leaf in response to the inoculation with AMF, with emphasis to A. longula, interests the pharmaceutical industry and can generate profit to the production of yellow passionfruit‐based anxiolytic herbal medicine. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Production of biomolecules of interest to the anxiolytic herbal medicine industry in yellow passionfruit leaves (Passiflora edulis f. flavicarpa) promoted by mycorrhizal inoculation

et al. 2019

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“…The main chemical compounds of the secondary metabolites of Tabernaemontana correspond to indolic alkaloids (Fumagali et al, 2008), a class of compounds with a wide spectrum of pharmacologic activities (Federici et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Morphoanatomic characterization of the stem and the leaf of Tabernaemontana catharinensis A.DC (Apocynaceae) and antimutagenic activity of its leaves

Guidoti

Rocha

Mourão

2015

Rev. bras. plantas med.

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Tabernaemontana catharinensis A. DC (Apocynaceae) is used as a medicinal plant by the population. In order to contribute to the safe use of the plant as herbal medicine, this study aimed to morphoanatomically characterize the aereal vegetative organs of T. catharinensis and to evaluate the leaves' mutagenic and antimutagenic activities. Histological blades of leaves and stem of T. catharinensis were performed; the methionine system (methG1) and Aspergillusnidulans conidia germination analysis were employed for mutagenic and antimutagenic evaluation. The morphoanatomic analysis did not show trichomes in the stem, petiole and leaf. Besides, it was observed both the presence of bi-collateral bundles -except in the foliar apex where the bundles were from the collateral type -as well as anamphistomatic leaf with paracyte stomata and sub-epidermal layer in the region of the leaf edges. The mutagenicity/ antimutagenicity trial indicated a significant decrease of mutation frequency in comparison with the control group and showed that theT. catharinensis had antimutagenic activity within the type, time and form of treatment. Since the germination test showed that the conidia germination was accelerated from the bud phase, activities at the cell cycle level and polarized growth proved to be possible. The morphoanatomic analysis of the leaf and stem associated with the mutagenic and antimutagenic analyses contributes to the safe use of the plant by humans and also for the quality control of a possible phytotherapeutic drug.Keywords: Aspergillus nidulans, genotoxicity, medicinal herbs, methionine system, morphoanatomy.RESUMO: Caracterização morfoanatômica do caule e da folha de Tabernaemontana catharinensis A.DC (Apocynaceae) e atividade antimutagênica de suas folhas Tabernaemontana catharinensis A. DC (Apocynaceae) é utilizada como planta medicinal pela população. A fim de contribuir para o uso seguro da planta como medicinal, este trabalho teve como objetivo caracterizar morfoanatomicamente os órgãos vegetativos aéreos de T. catharinensis e avaliar a atividade mutagênica e antimutagênica de suas folhas. Foram realizados cortes histológicos da folha e do caule de T. catharinensis e, para a avaliação mutagênica e antimutagênica, foi utilizado o sistema metionina (methG1) e análise da germinação de conídios em Aspergillus nidulans. A análise morfoanatômica evidenciou a ausência de tricomas no caule, pecíolo e folha; presença de feixes bicolaterais, com exceção no ápice foliar cujos feixes são do tipo colateral; folha anfiestomática com estômatos paracíticos e camada subepidérmica na região do bordo foliar. O ensaio de mutagenicidade/antimutagenicidade mostrou uma diminuição significativa da frequência de mutação em relação ao controle, indicando que nesse tipo, tempo e forma de tratamento, T. catharinensis apresentou atividade antimutagênica. O ensaio de germinação evidenciou que houve aceleração da germinação dos conídios, a partir da fase de botão, indicando uma possível atuação em nível de ativação de ciclo celu...

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“…According to FUMAGALI et al (2008), the production of large amounts of secondary metabolites by plant cell culture may occur within a few weeks, unlike the extraction of secondary metabolites of an annual or a perennial plant, which requires a longer time for accumulation of such metabolites.…”

Section: Total Phenols Contentmentioning

confidence: 99%

“…With the progress of biotechnology, the callus culture may be used for the in vitro biosynthesis of compounds of pharmaceutical interest (SANTOS, 2007), therefore reducing the indiscriminate exploitation of medicinal wild species. The production of such compounds is usually stimulated through a stress which can be chemical and/or physical (FUMAGALI et al, 2008). Growth regulators may affect the success of in vitro culture and auxins and cytokinins are widely used.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin

et al. 2015

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Produção de metabólitos secundários em cultura de células e tecidos de plantas: o exemplo dos gêneros Tabernaemontana e Aspidosperma

Cited by 58 publications

References 62 publications

Production of biomolecules of interest to the anxiolytic herbal medicine industry in yellow passionfruit leaves (Passiflora edulis f. flavicarpa) promoted by mycorrhizal inoculation

Production of biomolecules of interest to the anxiolytic herbal medicine industry in yellow passionfruit leaves (Passiflora edulis f. flavicarpa) promoted by mycorrhizal inoculation

Morphoanatomic characterization of the stem and the leaf of Tabernaemontana catharinensis A.DC (Apocynaceae) and antimutagenic activity of its leaves

Vitamin C and total phenols quantification in calli of native passion fruit induced by combinations of Picloram and Kinetin

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