Viability, production and morphology of pollen grains for different species in the genus Manihot (Euphorbiaceae)

Vieira, Lívia de Jesus; Soares, Taliane Leila; Rossi, Mônica Lanzoni; Alves, Alfredo Augusto Cunha; Santos, Francisco de Assis Ribeiro dos; Souza, Fernanda Vidigal Duarte

doi:10.1590/s0102-33062012000200011

Cited by 15 publications

(8 citation statements)

References 14 publications

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“…Vieira et al [25], studying pollen grains of accessions of Manihot spp. (cassava), found different results when they compared pollen viability by colorimetric methods and germination ones.…”

Section: Discussionmentioning

confidence: 99%

Viability and Action of CPL Lectin on in Vitro Germinability of Pollen Grains of Malpighia emarginata DC.—(Malpighiaceae)

Sousa¹,

Rego²,

Santos³

2013

AJPS

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This study aimed to observe the viability of the pollen grains of Malpighia emarginata DC. (West Indian cherry) and the action of a lectin in their germination. Lectins are proteins that specifically interact with carbohydrates, but don't modify them and bind with high affinity and specificity, promoting a transfer of information that is clearly central to many cellular processes in living beings. For the viability test was used aniline blue in lactophenol. The in vitro germination test used was the hanging drop method, in control medium containing sucrose, boric acid, calcium nitrate and agar. The experiment dealt with three different treatments in order to pollen germination: growing medium without addition of lectin, with 1.0 µg/ml and with 3.0 µg/ml CPL lectin. This lectin is extracted from seeds of Crotalaria pallida L.-Leguminosae. Data on pollen grains were analyzed by one-way ANOVA, with 95% reliability and comparison of averages by Turkey test at 5% probability. It was found that despite previous high viability of the pollen grains (c. 70%), a germinability rate has been low, yet exceptionally larger in pollen samples placed on a growing medium with addition of 3.0 µg/ml of CPL lectin (24%). There was no significant difference among the pollen grains germinate in medium without lectin and those in medium with the addition of only 1.0 µg/ml. According to data obtained in the treatments, CPL lectin, with concentration of 3.0 µg/ml, influenced the formation of the pollen tube and thus more pollen germinated in Malpighia emarginata.

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Section: Discussionmentioning

confidence: 99%

Viability and Action of CPL Lectin on in Vitro Germinability of Pollen Grains of Malpighia emarginata DC.—(Malpighiaceae)

Sousa¹,

Rego²,

Santos³

2013

AJPS

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“…esculenta, observed that the use of wild species as the male parent was more promising. Analogously, Vieira et al (2012) found that wild Manihot species generally produce pollen in greater quantity and with better (1)…”

Section: Resultsmentioning

confidence: 94%

“…This low fertilization rate could also be related to the "low" pollen viability reported for Manihot species, because pollen lasts for only 48 hours after anthesis (Halsey et al, 2008). According to Vieira et al (2012), the pollen remains viable for six days when conserved in calcium chloride, and loses its viability when stored fresh at room temperature. When creating hybrids between M. esculenta subsp.…”

Section: Rf (%)mentioning

confidence: 99%

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Obtaining hybrids of cultivars and wild subspecies of cassava

Silva

Martins

Santos

et al. 2018

Pesq. agropec. bras.

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-The objective of this work was to evaluate the crossability between cassava (Manihot esculenta subsp. esculenta) cultivars and the subspecies M. esculenta subsp. flabellifolia and M. esculenta subsp. peruviana. Plants from the BRS Jari, BRS Formosa, BRS Verdinha, and BGM 2050 (Equador 72) cultivars were crossed with the two subspecies. During flowering, at pre-anthesis, pistillate flowers were covered with a nylon bag and staminate flowers on the same inflorescence were emasculated. The staminate flowers were collected and placed in flasks previously labeled and sterilized with alcohol; some fresh flowers were stored at room temperature due to asynchronous flowering. The crosses were made between 10 a.m. and 2 p.m. After pollination, the flowers were covered again. Evaluations were made from the moment of pollination to the dehiscence of the fruit. Of the total flowers pollinated, 38% were fertilized, and 504 developed fruits and 816 produced seeds were observed. As a male parent, M. esculenta subsp. flabellifolia is more efficient in crosses with M. esculenta subsp. esculenta and may be recommend for controlled crosses. The crosses between the cassava cultivars and M. esculenta subsp. flabellifolia and M. esculenta subsp. peruviana produce viable seeds.Index terms: Manihot esculenta, controlled pollination, hybrid, plant breeding, wild relative species. Obtenção de híbridos de cultivares e de subespécies silvestres de mandiocaResumo -O objetivo deste trabalho foi avaliar o potencial de obtenção de híbridos oriundos do cruzamento de cultivares de mandioca (Manihot esculenta subsp. esculenta) com as subespécies M. esculenta subsp. flabellifolia e M. esculenta subsp. peruviana. Os cruzamentos foram realizados entre plantas das cultivares BRS Jari, BRS Formosa, BRS Verdinha e BGM 2050 (Equador 72) e das duas subespécies. Por ocasião do florescimento, no período pré-antese, as flores femininas foram cobertas com saco de pano tipo voal, com posterior emasculação das flores masculinas. As flores masculinas foram coletadas e acondicionadas em frascos previamente identificados e desinfetados com álcool; algumas flores foram armazenadas em temperatura ambiente devido ao florescimento assincrônico. Os cruzamentos foram feitos entre 10 e 14 horas. Após a polinização, as flores foram cobertas novamente. As avaliações foram efetuadas desde a polinização até a deiscência dos frutos. Do total das flores polinizadas, 38% foram fertilizadas, tendo-se observado 504 frutos desenvolvidos e 816 sementes produzidas. Manihot esculenta subsp. flabellifolia como parental masculino é mais eficiente em cruzamentos com M. esculenta subsp. esculenta e pode ser indicada para cruzamentos controlados. Os cruzamentos realizados entre as cultivares de mandioca e M. esculenta subsp. flabellifolia e M. esculenta subsp. peruviana propiciam a obtenção de sementes viáveis.Termos para indexação: Manihot esculenta, polinização controlada, híbrido, melhoramento de plantas, parentes silvestres.

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“…12. Micrografia de barrido de la especie cultivada (Manihot esculenta) (10 µm) (Vieira et al 2012). 13.…”

Section: Uso Y Función De La Cerámicaunclassified

Manejo y cultivo de plantas en sierras húmedas del NE de Brasil ca. 670-530 BP: evidencias palinológicas del yacimiento Evaristo I

et al. 2015

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paleoclimáticos debido a problemas tafonómicos que afectan la preservación polínica , sin embargo nos ofrecen a menudo información muy valiosa sobre las actividades humanas y las culturas prehistóricas (Dimbleby 1985; Holloway y Bryant 1986). El polen de plantas consumidas por antiguas poblaciones adherido a las vasijas cerámicas, piedras de moler u otros artefactos Los estudios arqueopalinológicos ayudan a la comprensión de los aspectos culturales de las poblaciones humanas en el pasado, especialmente a través de los cambios en la vegetación natural causados por la acción antrópica (Bryant y Hall 1993;Carrión et al. 2009

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Viability, production and morphology of pollen grains for different species in the genus Manihot (Euphorbiaceae)

Cited by 15 publications

References 14 publications

Viability and Action of CPL Lectin on <i>in Vitro</i> Germinability of Pollen Grains of <i>Malpighia emarginata</i> DC.—(Malpighiaceae)

Viability and Action of CPL Lectin on <i>in Vitro</i> Germinability of Pollen Grains of <i>Malpighia emarginata</i> DC.—(Malpighiaceae)

Obtaining hybrids of cultivars and wild subspecies of cassava

Manejo y cultivo de plantas en sierras húmedas del NE de Brasil ca. 670-530 BP: evidencias palinológicas del yacimiento Evaristo I

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Viability, production and morphology of pollen grains for different species in the genus Manihot (Euphorbiaceae)

Cited by 15 publications

References 14 publications

Viability and Action of CPL Lectin on &lt;i&gt;in Vitro&lt;/i&gt; Germinability of Pollen Grains of &lt;i&gt;Malpighia emarginata&lt;/i&gt; DC.—(Malpighiaceae)

Viability and Action of CPL Lectin on &lt;i&gt;in Vitro&lt;/i&gt; Germinability of Pollen Grains of &lt;i&gt;Malpighia emarginata&lt;/i&gt; DC.—(Malpighiaceae)

Obtaining hybrids of cultivars and wild subspecies of cassava

Manejo y cultivo de plantas en sierras húmedas del NE de Brasil ca. 670-530 BP: evidencias palinológicas del yacimiento Evaristo I

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Viability and Action of CPL Lectin on <i>in Vitro</i> Germinability of Pollen Grains of <i>Malpighia emarginata</i> DC.—(Malpighiaceae)

Viability and Action of CPL Lectin on <i>in Vitro</i> Germinability of Pollen Grains of <i>Malpighia emarginata</i> DC.—(Malpighiaceae)