Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha

Santa-Rosa, Sandra; Souza, Fernanda Vidigal Duarte; Vidal, Ádila Melo; Lêdo, Carlos Alberto da Silva; Santana, José Rf de

doi:10.1590/s0102-05362013000100018

Cited by 17 publications

(10 citation statements)

References 14 publications

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“…In general, 25ºC resulted in greater seedling growth, except for O. maculata seedlings, which exhibited lower growth using 25 to 100% salts at 18ºC (Table 1). Similar behavior was observed in A. blanchetiana, which maintained the lowest growth indices at 360 days at lower temperatures (SANTA-ROSA et al, 2013).…”

Section: Ms Salt Medium Reduction and Culture Temperaturesupporting

confidence: 76%

“…For these species, the use of low temperatures possibly increased the viability of the cultures by slowing the tissue degradation process (Table 1). Low temperature has been proven efficient for reducing the growth of several species and retaining greener leaves for a longer time, as observed in Aechmea blanchetiana (SANTA-ROSA et al, 2013) and potato (ARRIGONI-BLANK et al, 2014). However, low temperatures can also be limiting for other species, as observed in this study for C. macrocarpum, which had scores higher than 2, indicating over 50% of dead leaves (25ºC).…”

Section: Ms Salt Medium Reduction and Culture Temperaturementioning

confidence: 99%

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n vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil

Menezes¹,

Blank²,

Costa³

et al. 2019

Biosci. J.

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Orchids are known for the beauty, exuberance, and color of their flowers and therefore represent one of the most coveted ornamental plants. Due to their high commercial value, several illegal practices of removal and commercialization have led to the verge of extinction. This study aimed to develop protocols for in vitro conservation under slow growth and acclimatization of species of the subfamily Epidendroideae (Orchidaceae), from the state of Sergipe, Brazil. Two experiments were conducted: the first one tested four concentrations of MS (MURASHIGE and SKOOG, 1962) (25%, 50%, 75%, 100%), three orchid species (Catasetum macrocarpum, Oeceoclades maculata, and Polystachya estrellensis), and two temperatures (18 and 25ºC); the second one tested three combinations of carbon sources and osmotic regulators (20 g L -1 sucrose; 10 g L -1 sucrose + 5 g L -1 de mannitol; 10 g L -1 sucrose + 5 g L -1 sorbitol) and the same three species and two temperatures. For the in vitro conservation of C. macrocarpum, O. maculata, and P. estrellensis over 450 days, the use of 25% of MS salts or 20 g.L -1 sucrose at 25ºC is recommended. C. macrocarpum seedlings were acclimatized using sand + shredded pine bark + worm castings at a ratio of 2:2:1 (w:w:w). The use of sand alone is recommended for the acclimatization of O. maculata.

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Section: Ms Salt Medium Reduction and Culture Temperaturesupporting

confidence: 76%

Section: Ms Salt Medium Reduction and Culture Temperaturementioning

confidence: 99%

n vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil

Menezes¹,

Blank²,

Costa³

et al. 2019

Biosci. J.

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“…As far as bromeliads are concerned, this biotechnology method provides an efficient commercial tool for the reintroduction of the plants, a fact which is extremely important for endangered species (Dal Vesco et al, 2011). In the last two decades, several studies regarding bromeliads have been published (Silveira et al, 2009;Santa-Rosa et al, 2013). After in vitro propagation, different systems are used in plantlet acclimatization (Deb and Imchen, 2010), a critical micropropagation phase which represents a limiting factor for its success (Chandra et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

et al. 2016

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This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA 3 or 0.2 µM NAA, sucrose at 15 or 30 g L -1 , in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA 3 and 15 g L -1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L -1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.Keywords: enzymatic activity, oxidative stress, micropropagation, proline. Niveis de prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos durante o crescimento in vitro e aclimatização de Pitcairnia encholirioides L.B. Sm. (Bromeliaceae) ResumoEste trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA 3 ou 0,2 µM de ANA, 15 ou 30 g L -1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA 3 e 15 g L -1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L -1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.Palavras-chave: atividade enzimática, estresse oxidativo, micropropagação, prolina.

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“…The positive effect of the NAA on the regeneration of shoots from the stem explants was demonstrated by SANTA-ROSA et al (2013), as well as for the bromeliad Aechmea distichantha, in which the best results were seen in response to the highest concentration they used (0.5 μM). et al (2007a) in their study on Billbergia distachia stem explants reported 5.3 shoots, on average, in response to the combined action of the 5 µM BAP and 3 µM NAA applied.…”

Section: Resultsmentioning

confidence: 88%

Micropropagation of Chapada Diamantina ornamental bromeliad

2020

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Sincoraea mucugensis (Wand. & A.A. Conc.) LOUZADA & WAND, an endangered bromeliad, is confined to the central region of the Chapada Diamantina, in the municipality of Mucugê, Brazil. From various researches, it is evident that for the propagation of this species, the in vitro technique is a feasible option. However, due to the low multiplication rates reported in various papers, this study aimed to establish a micropropagation protocol of direct organogenesis for S. mucugensis. First, the inoculation of the stem explants was done in MS ½ culture medium which contained different levels of BAP (0.00; 6.66; 8.88; 11.10; 13.20 µM) and NAA (0.00; 2.60; 5.20 µM). These shoots were then subjected to a couple of distinct rooting periods (of 30-and 60-day duration) using activated charcoal; finally, these microplants were transferred to a greenhouse for acclimatization, and covered with transparent plastic cups, as a water loss prevention test method. All the data were submitted to the analysis of variance (ANOVA), and the means were subjected to regression analysis or compared using the Tukey test. The findings revealed that the S. mucugensis stem explants raised in the NAA-rich medium (6.42 to 7.43 shoots/explants) showed high multiplication rates; the shoot rooting was done for 30 days using activated charcoal with the medium. Acclimatization, which was performed by directly exposing the microplants to the ex vitro environment, showed 95% survival rate. RESUMO: Sincoraea mucugensis (Wand. & A.A. Conc.) LOUZADA & WAND é uma bromélia vulnerável de ocorrência restrita ao município de Mucugê, Chapada Diamantina. Estudos indicam que a cultura de tecidos é uma alternativa viável para a propagação in vitro destaespécie. Contudo, em função das baixas taxas de multiplicação obtidas em estudos anteriores, objetivou-se estabelecer um protocolo de micropropagação via organogênese direta para S. mucugensis. Explantes caulinares foram inoculados em meio de cultura MS ½ contendo diferentes concentrações de BAP (0,00; 6,66; 8,88; 11,10; 13,20 µM) e ANA (0,00; 2,60; 5,20 µM). Os brotos obtidos foram submetidos a diferentes períodos de enraizamento (30 e 60 dias) com carvão ativado; posteriormente as microplantas foram aclimatizadas em casa de vegetação, testando-se o efeito da cobertura com copos plásticos transparentes como estratégias contra perda de água. Os dados foram submetidos à análise de variância (ANOVA) e as médias analisadas por regressão ou comparadas pelo Teste de Tukey. Os resultados demonstram que altas taxas de multiplicação de S. mucugensis são geradas a partir de explantes caulinares cultivados em meio contendo ANA (6,42 a 7,43 brotos/explantes); o enraizamento dos brotos é realizado por 30 dias em meio com carvão ativado e a aclimatização é feita com exposição direta das microplantas ao ambiente ex vitro com 95% de sobrevivência. Palavras-chave: propagação in vitro, aclimatização, Sincoraea mucugensis, ANA.

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Micropropagation of the ornamental vulnerable bromeliads Aechmea blanchetiana and Aechmea distichantha

Cited by 17 publications

References 14 publications

n vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil

n vitro conservation and acclimatization of Epidendroideae (Orchidaceae) from Sergipe, Brazil

Proline levels, oxidative metabolism and photosynthetic pigments during in vitro growth and acclimatization of Pitcairnia encholirioides L.B. Sm. (Bromeliaceae)

Micropropagation of Chapada Diamantina ornamental bromeliad

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