Atividade antioxidante e viabilidade de sementes de girassol após estresse hídrico e salino

Carneiro, Marília Mércia Lima Carvalho; Deuner, Sidnei; Oliveira, Pablo Valadão de; Teixeira, Sheila Bigolin; Pinho, Camila Ferreira de; Bacarin, Marcos Antônio; Moraes, Dário Munt de

doi:10.1590/s0101-31222011000400017

Cited by 25 publications

(21 citation statements)

References 24 publications

(16 reference statements)

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“…The same behavior is expected for CAT enzyme activity since it acts on the products processing derived from SOD activity. This increased activity of both enzymes during the germination process has been reported in a study by Flores et al (2014) with seeds of Melanoxylon brauna, where they observed increased activity of the two enzymes with longer exposure of the seeds to imbibition, and by Carneiro et al (2011), with sunflower seeds in NaCl solutions.…”

Section: Introductionsupporting

confidence: 79%

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

et al. 2017

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ABSTRACT. With the imminence of global climate changes that affect the temperature and the rainfall uniformity, it is growing the concern about the adaptation of crops to the water deficit. Thus, the objective of this study was to evaluate alterations in physiological and enzymatic mechanisms during the germination process of sesame seeds under different water availability. To simulate the water restriction we used PEG6000, a high molecular weight molecule that does not penetrate the seed structure but allows different osmotic potentials. The treatments were -0.1, -0.2, and -0.3 MPa, and the control. Germination, first-count germination, germination velocity index, and length and dry mass of the hypocotyl and radicle were performed. The seeds were weighed before and after treatments every 3 h. After each weighing, 100 seeds were taken for analysis of the enzymes alcohol dehydrogenase (ADH), malate dehydrogenase, esterase, catalase (CAT), superoxide dismutase (SOD), isocitrate lyase (ICL), and glutamate dehydrogenase (GTDH). The statistical design was completely randomized with five replications. PEG6000 prolonged ADH activity during the beginning of germination, maintaining the anaerobic metabolism for longer. Subsequently, their activity was reduced, as well as ICL, favoring the deterioration of the seeds that take the time to germinate. Behavior was evidenced by the appearance of SOD, CAT, and GTDH isoforms after 24 h of imbibition when water restriction was imposed. Therefore, the PEG600 is efficient in simulating water deficit conditions in future scenarios of climate change, offering impotent information regarding the germination behavior of the plants under these conditions.

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Section: Introductionsupporting

confidence: 79%

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

et al. 2017

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“…Thus, a reduction of the activity of this enzyme may result in a decrease of oxidative injuries to seeds, which can explain the results found in this study on castor oilseeds. Carneiro et al (2011) also observed a decrease in the catalase activity associated with loss of viability of sunfl ower seeds. Therefore, this evaluation suggests that catalase can be a deterioration marker for castor plant seeds.…”

Section: Resultsmentioning

confidence: 76%

Physiological and biochemical aspects of castor beans seeds deterioration stored in different packaging conditions and temperatures

Santos¹,

Carvalho²,

Caldeira³

et al. 2016

J. Seed Sci.

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-The present study aimed to evaluate effects of different storage conditions on the castor bean seed cultivar IAC-226 quality, stored for 12 months. For this purpose, seeds were stored in different environment and packaging conditions: in a cold chamber and conventional storage, using multiwall Kraft paper and in no vacuum and vacuum plastic packages at 1 atm; as well as under cryopreservation storage (-196 °C). Seed quality was evaluated before and after 4, 8 and 12 months of storage by germination tests, first count of germination, emergence percentage, emergence speed index and determination of changes in catalase (CAT) and superoxide dismutase (SOD) enzyme systems. Cryopreservation (-196 °C) is efficient in maintaining the physiological quality of castor bean cultivar IAC-226 for 12 months. The enzyme catalase stands out as a marker of castor seed deterioration during storage.Index terms: Ricinus communis L. storage, cryopreservation, vacuum.Aspectos bioquímicos e fisiológicos da deterioração de sementes de mamona armazenadas em diferentes embalagens e temperaturas RESUMO -Objetivou-se avaliar os efeitos de diferentes condições de armazenamento sobre a qualidade de sementes de mamona cultivar IAC-226, armazenadas por um período de 12 meses. Para isso, as sementes foram armazenadas em diferentes ambientes e tipos de embalagens: em câmara fria e armazém convencional, utilizando embalagens papel Kraft multifoliado e plástico com e sem acondicionamento a vácuo a 1 atm; e também armazenamento sob criopreservação (-196 °C). A qualidade das sementes foi avaliada antes e após 4, 8 e 12 meses de armazenamento pelos testes de geminação, primeira contagem de germinação, porcentagem de emergência, índice de velocidade de emergência e pela determinação das alterações nos sistemas enzimáticos catalase (CAT) e superóxido dismutase (SOD). A criopreservação (-196 °C) é eficiente na manutenção da qualidade fisiológica de sementes de mamona cultivar IAC-226 por 12 meses. A enzima catalase se destaca como um marcador da deterioração de sementes de mamona durante o armazenamento.Termos para indexação: Ricinus communis L., isoenzimas, criopreservação, armazenamento.1

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“…Reduced expression levels of catalase were also found at 25 ºC for AP8202 and AP8203 hybrids. The antioxidant enzyme systems of seeds are an important primary defense against free radicals generated under stress conditions, such as superoxide dismutase (SOD), which catalyzes the dismutation of the superoxide radical in H 2 O 2 and O 2; catalase (CAT) and ascorbate peroxidase (APX), which can break H 2 O 2 in H 2 O and O 2 (Carneiro et al, 2011). The regulation of these antioxidant enzymes can be lost if stress is too severe, greatly increasing the production of free radicals that can lead to a cascade of events that begins with lipid peroxidation, advancing towards membrane degradation and cell death (Greggains et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Physiological, isozyme changes and image analysis of popcorn seeds submitted to low temperatures

Catão

Caixeta

2017

J. Seed Sci.

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-This present study aimed to assess seed quality of popcorn seedlings subjected to low temperatures by examining their physiological changes, enzymes, and images. We used a completely randomized design with four replications in a factorial arrangement with four hybrids (P618, AP6002, AP8202, AP8203) and five temperatures (10, 13, 16, 19 and 25 °C). Their physiological quality was assessed through radicle protrusion, germination, dry mass of seedlings and emergence. We have assessed the images of seedlings through their hypocotyl and root lengths; and through rates of automatic force, uniformity and growth, provided by Groundeye. The isozyme expressions were determined for the catalase (CAT -IUBMB: EC 1.11.1.6), alcohol dehydrogenase (ADH -IUBMB: EC 1.1.1.1), malate dehydrogenase (MDH -IUBMB: EC: 1.1.1.37) and α-amylase (α-AMY -IUBMB: EC 3.2.1.1). Results show that low temperatures cause a negative effect on seed physiological quality in enzyme expression of CAT, ADH, MDH and α-AMY and on the performance of seedlings. These alterations compromise seed quality. Hybrids AP6002 and AP8203 should be recommended for sowing at up to 16 °C. The Groundeye software was efficient in the analysis of popcorn seedlings and in for the assessment of seed quality when submitted to low temperatures.

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Atividade antioxidante e viabilidade de sementes de girassol após estresse hídrico e salino

Cited by 25 publications

References 24 publications

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

Research Article Physiological and enzymatic alterations in sesame seeds submitted to different osmotic potentials.

Physiological and biochemical aspects of castor beans seeds deterioration stored in different packaging conditions and temperatures

Physiological, isozyme changes and image analysis of popcorn seeds submitted to low temperatures

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