Genetic purity certificate in seeds of hybrid maize using molecular markers

Salgado, Kalinka Carla Padovani de Carvalho; Vieira, das Graças Guimarães Carvalho; Pinho, Édila Vilela de Resende Von; Guimarães, C. T.; Pinho, Renzo Garcia Von; Souza, Lourenço Viana de

doi:10.1590/s0101-31222006000100024

Cited by 18 publications

(14 citation statements)

References 11 publications

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“…Use of SSR markers has allowed distinguishing among accessions of the cultivated peanut species, A. hypogaea L. (Kottapalli et al, 2007); this was not possible with RFLP or RAPD markers Halward et al, 1991). The use of microsatellite markers for assessing true hybrids is common in tomato (Smith and Register, 1998), maize (Salgado et al, 2006), rice (Yashitola et al, 2002), and cotton (Dongre and Parkhi, 2005).…”

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confidence: 99%

Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis

Gomez¹,

Denwar²,

Ramasubramanian³

et al. 2008

Peanut Science

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In peanut hybridization, distinguishing inadvertent selfs from the true hybrids may be difficult. In this study, to differentiate between selfs and hybrids, DNA was extracted from leaf tissue of F 1 or F 2 plants, and SSR markers were amplified and bands separated by a novel submarine horizontal polyacrylamide gel electrophoresis (H-PAGE). By comparing the resulting banding patterns to those of the parents, 70% of the putative hybrids were shown to be true hybrids on the basis of possessing a marker allele from the male parent. The H-PAGE gels gave better band separation and differentiation of selfed progenies than agarose gels, and were compatible with the common horizontal agarose gel units. This method provides a quick assay to distinguish hybrids from inadvertent selfs, and should result in greater efficiency and more effective use of resources in peanut breeding programs.

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confidence: 99%

Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis

Gomez¹,

Denwar²,

Ramasubramanian³

et al. 2008

Peanut Science

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“…Self-pollination of female parent is one of the main reasons for contamination in hybrid production. This is as result of incomplete removal of its tassel (Salgado et al 2006). Also, the purity level of parental inbred lines could affect the purity of their hybrids.…”

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confidence: 99%

Genetic analysis of maize (Zea mays L.) hybrids using microsatellite markers

HANÇER¹

2015

Tarım Bilimleri Dergisi

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Genetic purity is one of the most important quality criteria required for successful hybrid seed production. In this study, molecular markers were used for assessing the genetic purity and diversity of three commercially important maize F 1 hybrids (Pasha, Frida and PG1661) and their parental inbred lines. Fifty Simple Sequence Repeats (SSRs) markers were used to analyze samples, also the efficiency of the markers were compared. Twenty three primer pairs among the fifty markers were able to detect polymorphism between the different types of hybrids with an average of 0.69 polymorphism information content (PIC) value. Genetic purity analyses revealed more than 98% homogeneity in the hybrid seeds. The hybrids were grouped into three main clusters. It can be concluded that, molecular markers are efficient to study the genetic purity and diversity in maize hybrids and microsatellites are more accurate marker-type because of their co-dominancy. ÖZETGenetik saflık, hibrit tohum üretimi için gerekli olan önemli kalite kriterlerinden biridir. Bu çalışmada, moleküler işaretleyiciler ticari olarak önemli 3 mısır F 1 hibrit çeşidi (Pasha, Frida ve PG1661) ve kendilenmiş saf ebeveyn hatlarının genetik saflık ve çeşitlilik analizleri için kullanılmıştır. Örnekler, elli adet basit dizi tekrarları (SSR) işaretleyicileri kullanılarak analiz edilmiş ve işaretleyicilerin verimliliği karşılaştırılmıştır. Testlenen 50 işaretleyici içinden 23 primer çifti ortalama 0.69 polimorfizm bilgisi değeri ile değişik hibrit çeşitleri arasındaki farklılığı tespit etmiştir. Hibrit tohumların genetik saflık analizi, % 98'den yüksek homoloji oranı ile sonuçlanmıştır. Hibritler, 3 ana gruba toplamıştır. Bu çalışma ile moleküler işaretleyicilerin mısır hibritlerinin genetik saflık ve çeşitlilik analizleri için kullanılmasının verimli olduğu ve mikrosatellitlerin ko-dominantlık özellikleri nedeni ile doğru işaretleyiciler olduğu saptanmıştır.

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“…Segundo Salgado et al (2006), a escolha do estádio para análise da pureza genética depende do objetivo da avaliação. Pinho et al (1997) concluíram que é mais Ciênc.…”

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Identificação de cultivares e certificação da pureza genética de gladíolo por meio de marcadores morfológicos

et al. 2011

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RESUMOA caracterização de cultivares de flores é fundamental nos processos de registro e proteção dessas e também na certificação da pureza genética. Nesse trabalho foram realizadas a caracterização e a certificação da pureza genética de cultivares de Gladiolus sp. por meio de marcadores morfológicos. Utilizaram-se onze cultivares de Gladiolus sp., e para a caracterização das cultivares, as parcelas foram constituídas de 25 plantas avaliando-se características dos cormos, folhas e flores. O delineamento experimental adotado foi de blocos casualizados, com quatro repetições Para a certificação da pureza genética, foram instaladas parcelas constituídas de 24 plantas e cormos contendo contaminação varietal em diferentes proporções. Foram utilizadas três cultivares para compor os tratamentos e as avaliações foram conduzidas por três avaliadores que desconheciam a porcentagem existente de contaminação varietal. Foram calculadas as porcentagens de acerto e de erro com base nos resultados obtidos nas avaliações. Foi calculado o desvio padrão da média dos resultados nas quatro repetições, obtidos pelos três avaliadores. Para verificar a significância dos desvios entre os resultados obtidos pelos avaliadores foi utilizado o teste de qui-quadrado ( 2 ). Por meio de características do cormo é possível diferenciar quatro cultivares das onze avaliadas. A característica da cor das flores é o descritor morfológico mais seguro para a diferenciação das cultivares de gladíolo. Dependendo das características das cultivares contaminantes e das cultivares em análises é possível utilizar descritores morfológicos para a certificação de pureza genética em cultivares de gladíolo.Termos para indexação: Gladiolus sp, flores, contaminação varietal. ABSTRACTThe characterization of flower cultivars is fundamental in the registration and protection processes and also in the genetic purity certification. In this work, both the characterization and the genetic purity certification of cultivars of Gladiolus sp by means of morphologic markers were made. Eleven cultivars of Gladiolus sp. were used and for the characterization of the cultivars, the plots were made up of 25 plants, evaluating the characteristics of corms, leaves and flowers. The used experimental design was the one of randomized blocks with four replicates. For the genetic purity certification, plots with 24 plants and corms containing varietal contaminations in different proportions were established. Three cultivars were used to make up the treatments, and the evaluations were conducted by three evaluators who were unaware of the existent percentage of varietal contamination. The percentage of mistakes was calculated based upon the obtained results of the evaluations. The standard deviation of the means of the results in the four replicates obtained by the three evaluators was calculated. In order to verify the significance of the deviations among the obtained results by the evaluators, Chi-square test ( 2 ) was used. By means of corm characteristics, it is possible to distinguish ...

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Genetic purity certificate in seeds of hybrid maize using molecular markers

Cited by 18 publications

References 11 publications

Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis

Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis

Genetic analysis of maize (Zea mays L.) hybrids using microsatellite markers

Identificação de cultivares e certificação da pureza genética de gladíolo por meio de marcadores morfológicos

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