Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis

Abstract

In peanut hybridization, distinguishing inadvertent selfs from the true hybrids may be difficult. In this study, to differentiate between selfs and hybrids, DNA was extracted from leaf tissue of F 1 or F 2 plants, and SSR markers were amplified and bands separated by a novel submarine horizontal polyacrylamide gel electrophoresis (H-PAGE). By comparing the resulting banding patterns to those of the parents, 70% of the putative hybrids were shown to be true hybrids on the basis of possessing a marker allele from the male parent. The H-PAGE gels gave better band separation and differentiation of selfed progenies than agarose gels, and were compatible with the common horizontal agarose gel units. This method provides a quick assay to distinguish hybrids from inadvertent selfs, and should result in greater efficiency and more effective use of resources in peanut breeding programs.

Inheritance of fresh seed dormancy in Spanish-type peanut (Arachis hypogaea L.): bias introduced by inadvertent selfed flowers as revealed by microsatellite markers control

“…This percentage of true F 1 hybrids indicated most likely that inadvertent selfs were collected at harvest, although caution was taken to remove underground uncolored flowers till 60 days after emergency. The average percentage of true F 1 hybrids found in this study was lower than the percentage (60 -70%) observed by Gomez et al (2008). However, percentage of true hybrids in self-pollinated crops depends on the female parent used in the cross since the number of underground flowers may vary from one cultivar to another.…”

Identification of QTLs for pod and kernel traits in cultivated peanut by bulked segregant analysis

“…A 10 min extension was performed at 72ºC as the last step. Amplified products were analyzed using 6% (19:1, acrylamide: bisacrylamide) non-denaturing polyacrylamide gel (Gomez et al 2008). Electrophoresis was performed at 350 volts DC for 3.5 hrs in a Mega-gel High Throughput Vertical Unit (CBS Scientific, Del Mar, CA).…”

“…Markers for resistance to root-knot nematode (Garcia et al 1995;Burow et al 1996;Choi et al 1999), late leaf spot (Stalker and Mozingo, 2001;Mondal et al 2005;Mace et al 2006), the aphid vector causing groundnut rosette disease (Herselman et al 2004), seed infection by Aspergillus flavus (Yong et al 2005) and early leaf spot (Burow et al 2008) have been indentified. However, no markers have been identified for pod-and kernel-related traits.…”

Identification of true hybrid progenies in cassava using simple sequence repeat (SSR) markers

“…markers in rice (Yashitola et al 2002, Hashemi et al 2009), cotton (Wu et al 2001, Asif et al 2009, Dongre et al 2011, peanut (Gomez et al 2008), sugarcane (Zhang et al 2009), sunflower (Iqbal et al 2010) and cabbage (Liu et al 2007). Bianco et al (2011) used ISSR markers for identification of F 1 hybrids in artichoke and suggested that morphological trait alone is not sufficient to evaluate the offspring precisely.…”