Comparison of capillary electrophoresis and high performance liquid chromatography methods for caffeine determination in decaffeinated coffee

Abstract

Decaffeinated coffee accounts for 10 percent of coffee sales in the world; it is preferred by consumers that do not wish or are sensitive to caffeine effects. This article presents an analytical comparison of capillary electrophoresis (CE) and high performance liquid chromatography (HPLC) methods for residual caffeine quantification in decaffeinated coffee in terms of validation parameters, costs, analysis time, composition and treatment of the residues generated, and caffeine quantification in 20 commercial samples. Both methods showed suitable validation parameters. Caffeine content did not differ statistically in the two different methods of analysis. The main advantage of the high performance liquid chromatography (HPLC) method was the 42-fold lower detection limit. Nevertheless, the capillary electrophoresis (CE) detection limit was 115-fold lower than the allowable limit by the Brazilian law. The capillary electrophoresis (CE) analyses were 30% faster, the reagent costs were 76.5-fold, and the volume of the residues generated was 33-fold lower. Therefore, the capillary electrophoresis (CE) method proved to be a valuable analytical tool for this type of analysis.

Larvicidal Activity against Aedes aegypti and Chemical Characterization of the Inflorescences of Tagetes patula

“…Comparing the HPLC and CE methods developed for evaluation of BF of T. patula , CE was more efficient, being almost four times faster. In addition, in the CE method, organic solvents are not used to separate the analytes, and the volume of electrolytic solution used for analyses is small, making the technique less costly and polluting [ 39 – 41 ].…”

Bioactive Micronutrients in Coffee: Recent Analytical Approaches for Characterization and Quantification

“…In the last five years, CE has been applied in many fields including the analysis of plant materials [ 86 ]. While HPLC did provide lower values of LOD for the determination of caffeine in decaffeinated coffee than CE, the latter was 30% faster, had lower cost of reagents, and generated smaller volumes of residues [ 137 ]. In a similar experiment, a fast hydrodynamic injection (50 mBar, 7 s) was applied in the analysis of caffeine in commercial decaffeinated coffee using a 48 cm × 50 μ m fused-silica capillary [ 138 ].…”

Development of a method for simultaneous analysis of caffeine and taurine in energy drinks by micellar electrokinetic chromatography with diode-array detector

“…Both caffeine and taurine content are usually determined by high-performance liquid chromatography with diode array detector (HPLC-DAD) (Bizzotto et al, 2013;Chirita Tampu et al, 2018;Paula Lima & Farah, 2019;Rai et al, 2016) and capillary electrophoresis, mainly zone or micellar electrokinetic chromatography (MEKC) with diode array detector (DAD), is also often employed. However, taurine does not absorb light in the UV -VOS region, being detected indirectly, either by DAD, or by laser -induced fluorescence, amperometric and conductometric detectors (Rai et al, 2016;Sawabe et al, 2008;Vochyánová et al, 2014;Zinellu et al, 2009).…”