Ultrastructural analysis of in vitro direct and indirect organogenesis

Appezzato‐da‐Glória, Beatriz; Machado, Sílvia Rodrigues

doi:10.1590/s0100-84042004000300004

Cited by 9 publications

(14 citation statements)

References 31 publications

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“…These cells probably have repeated divisions, forming compact tissue without intercellular spaces. Similar ultrastructural features were found in cells in the meristemoids of plants during organogenesis [ 10 45 ]. Other cell types, highly vacuolated, with intercellular spaces in the NCs, may be related to the friability of these structures.…”

Section: Discussionsupporting

confidence: 59%

“…According to Fortes and Pais [ 9 ], an accumulation of starch in prenodular structures during organogenesis in H. lupulus (Cannabaceae) results from the high energy requirement of this process. Thus, the presence of starch granules in the plastids found in dividing cells seems to be a typical feature of organogenesis [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

“…In orchids, Lee et al [ 47 ] reported structures composed of compact and loose aggregates of cells, which then resulted in protocorm-like bodies. Nevertheless, meristemoids in Bauhinia forficata (Fabaceae) and Glycine max (Fabaceae) did not originate from typical meristematic cells, but rather from highly vacuolated cells, which divide unequally to produce different-sized daughter cells [ 45 ]. Therefore, the two cell types observed in the NCs are important to achieving high regenerative potential, culminating in the multiple production of adventitious shoots.…”

Section: Discussionmentioning

confidence: 99%

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Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Corredor-Prado

Schmidt

Guerra

et al. 2015

J Microsc Ultrastruct

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Micropropagation via induction, multiplication and development of nodular cultures (NCs) is an efficient regeneration system for Bromeliaceae, a family of endangered monocot plants with ornamental value. Therefore, the present work aimed to induce NCs from seeds and leaf explants of Vriesea in order to characterize the morphological and histochemical aspects of induction and formation of these cultures. Seeds of Vriesea friburgensis var. paludosa were sterilized and inoculated into liquid culture media supplemented with different concentrations and combinations of growth regulators. Leaf explants of Vriesea reitzii were inoculated into medium supplemented with 4 μM α-naphthalene acetic acid (NAA) and 2 μM 6-benzylaminopurine (BAP). The addition of NAA (4 μM) in the culture medium used for seeds led to an induction rate of 72% in NCs. First, the embryo began to germinate, and afterwards, nodular structures started to form. While NCs formed from seeds is associated with root and shoot meristems, the formation of NCs from leaf explants involves the intercalary meristem. Meristematic cells generate an appropriate response in the induction medium, producing NCs by the proliferation of small cells with meristematic characteristics and large vacuolated cells. These results provide a better understanding of morphogenetic responses in bromeliads and, hence, the opportunity to develop optimized micropropagation protocols.Abbreviations: BAP, 6-benzylaminopurine; 2-iP, N6 (2-isopentyl) adenine; CBB, Coomassie Brilliant Blue; CLSM, confocal laser scanning microscopy; MSB, MS basal medium; NAA, α-Naphthalene acetic acid; NCs, nodular cultures; PAS, Periodic Acid-Schiff; SEM, scanning electron microscopy; TDZ, N-phenyl-N’-1,2,3-thidiazol-5-ylurea; TB-O, Toluidine Blue O; TEM, Transmission electron microscopy.

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Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Corredor-Prado

Schmidt

Guerra

et al. 2015

J Microsc Ultrastruct

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“…Ceres, Viçosa, v. 62, n.4, p. 340-346, jul-ago, 2015 amido. Mitocôndrias dessas células são abundantes, usualmente ovais ou arredondadas e apresentam uma matriz eletrodensa com a crista bem desenvolvida (Appezzatoda- Glória & Machado, 2004;Pinto et al, 2011), como as observadas, neste trabalho, em células de calos cultivados em meio com 2,4-D e cinetina. Essas características são típicas de intensa atividade metabólica, graças às altas taxas respiratórias.…”

Section: Resultsunclassified

Indução, análises morfológicas e ultraestruturais de calos de maracujazeiro nativo

et al. 2015

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A análise de calos que apresentem características embriogênicas é importante para posterior regeneração, in vitro, de espécies com características agronômicas desejáveis, como o maracujazeiro nativo Passiflora gibertii. Diante do exposto, objetivou-se, com este trabalho, analisar a indução de calos oriundos de explantes foliares de Passiflora gibertiiN. E. Brown, bem como caracterizá-los, morfológica e ultraestruturalmente. Para obtenção de calos, folhas cotiledonares foram inoculadas, em meio de cultura, suplementado com picloram e 2,4-D, combinados com cinetina. Após 30 dias em meio de cultura, no escuro, os calos obtidos foram preparados para a visualização em microscopia eletrônica (transmissão e varredura) e microscopia de luz. Os resultados permitem afirmar que a adição de picloram e cinetina ao meio de cultura promove maior formação de calos em explantes foliares de P. gibertii que 2,4-D e cinetina. O regulador 2,4-D proporciona a obtenção de calos com células de formato isodiamétrico, pequenas e com pequeno espaço intercelular, sistema celular organizado e predominância de mitocôndrias de formato arredondado. Já com a utilização do regulador de crescimento picloram, observa-se a predominância de células grandes e de formato alongado, de espaços intercelulares, de sistema celular desorganizado e de mitocôndrias de formato alongado.

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“…In B. forficata, two studies exist. Shoots have been shown to originate from cotyledonary node epidermal and subepidermal tissue through a process of indirect organogenesis and amitosis (nuclear fragmentation) (Appezzato-da- Glória and Machado 2004). In that study, half-strength MS (½ MS) basal medium to which 3% (w/v) sucrose and 4 mg·dm -3 BA were added allowed callus to form from 1 cm long hypocotyls after 30 days when cultured under 30 μmol·m -2 ·s -1 PPFD with a 16-h ____________________________________________________________________________________________________________________ photoperiod.…”

Section: Species-by-species Descriptionmentioning

confidence: 99%

A Review of the in Vitro Propagation of Bauhinia Spp

Silva¹

2013

Journal of Horticultural Research

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Bauhinia species (including B. acuminata, B. variegata, B. purpurea, B. monandra, B. galpinii, B. blakeana and B. acuminata) are popular ornamental plants, usually woody ornamentals or herbaceous lianas, with attractive flowers typical of the Leguminosae of arid, temperate, sub-tropical and tropical zones. Bauhinia species also serve as fodder and many have multiple medicinal and biological properties. There is an interest in commerce and amongst collectors to clonally propagate species from this genus. This review highlights protocols that currently exist for the in vitro culture of Bauhinia species as a means to clonally propagate material.

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Ultrastructural analysis of in vitro direct and indirect organogenesis

Cited by 9 publications

References 31 publications

Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Histodifferentiation and ultrastructure of nodular cultures from seeds of Vriesea friburgensis Mez var. paludosa (L.B. Smith) L.B. Smith and leaf explants of Vriesea reitzii Leme & A. Costa (Bromeliaceae)

Indução, análises morfológicas e ultraestruturais de calos de maracujazeiro nativo

A Review of the in Vitro Propagation of Bauhinia Spp

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