Phylogenetic characterization of serum plus antibiotic-resistant extraintestinal Escherichia coli obtained from the liver of poultry carcasses in Pernambuco
Abstract:RESUMO.-[Caracterização filogenética de Escherichia coli extraintestinal soro-e antibi ótico-resistentes isoladas do fígado de carcaças de frango em Pernambuco.] Neste estudo, isolados de Escherichia coli extraintestinal aviária obtidos a partir do fígado de carcaças de aves aprovadas para consumo humano no Estado de Pernambuco--Brasil foram testados para resistência a antibióticos e soro. As amostras de fígado (n = 110) foram obtidas de um abatedouro, sendo 88 isolados bacterianos identificados como Escherichi… Show more
“…Low resistance frequency to sulphamethoxazole-trimethoprim was also reported in this study, contrasting with most data [59][60][61]66]. Among aminoglycosides, high resistance prevalence to streptomycin is widespread, but not to other drugs of this class [61][62][63]67]. Colistin and polymyxin B resistance were rarely described [59].…”
Section: E Colicontrasting
confidence: 81%
“…The percentage of resistance to tetracycline was moderate (33%), but always associated with multidrug resistance [65]. Vaz et al (2017) in Pernambuco State (NE) studied E. coli strains isolated from poultry liver carcasses and detected multidrug resistance with frequencies up to 48%, varying according to the farm [67]. Borzi et al (2017) studied E. coli recovered from free-range helmeted guinea fowl in São Paulo State (SE), and multidrug resistance was detected in 90.4% of the isolates [62].…”
In animal husbandry, antimicrobial agents have been administered as supplements to increase production over the last 60 years. Large-scale animal production has increased the importance of antibiotic management because it may favor the evolution of antimicrobial resistance and select resistant strains. Brazil is a significant producer and exporter of animal-derived food. Although Brazil is still preparing a national surveillance plan, several changes in legislation and timely programs have been implemented. Thus, Brazilian data on antimicrobial resistance in bacteria associated with animals come from official programs and the scientific community. This review aims to update and discuss the available Brazilian data on this topic, emphasizing legal aspects, incidence, and genetics of the resistance reported by studies published since 2009, focusing on farm animals and derived foods with the most global public health impact. Studies are related to poultry, cattle, and pigs, and mainly concentrate on non-typhoid Salmonella, Escherichia coli, and Staphylococcus aureus. We also describe legal aspects of antimicrobial use in this context; and the current occurrence of genetic elements associated with resistance to beta-lactams, colistin, and fluoroquinolones, among other antimicrobial agents. Data here presented may be useful to provide a better understanding of the Brazilian status on antimicrobial resistance related to farm animals and animal-derived food products.
“…Low resistance frequency to sulphamethoxazole-trimethoprim was also reported in this study, contrasting with most data [59][60][61]66]. Among aminoglycosides, high resistance prevalence to streptomycin is widespread, but not to other drugs of this class [61][62][63]67]. Colistin and polymyxin B resistance were rarely described [59].…”
Section: E Colicontrasting
confidence: 81%
“…The percentage of resistance to tetracycline was moderate (33%), but always associated with multidrug resistance [65]. Vaz et al (2017) in Pernambuco State (NE) studied E. coli strains isolated from poultry liver carcasses and detected multidrug resistance with frequencies up to 48%, varying according to the farm [67]. Borzi et al (2017) studied E. coli recovered from free-range helmeted guinea fowl in São Paulo State (SE), and multidrug resistance was detected in 90.4% of the isolates [62].…”
In animal husbandry, antimicrobial agents have been administered as supplements to increase production over the last 60 years. Large-scale animal production has increased the importance of antibiotic management because it may favor the evolution of antimicrobial resistance and select resistant strains. Brazil is a significant producer and exporter of animal-derived food. Although Brazil is still preparing a national surveillance plan, several changes in legislation and timely programs have been implemented. Thus, Brazilian data on antimicrobial resistance in bacteria associated with animals come from official programs and the scientific community. This review aims to update and discuss the available Brazilian data on this topic, emphasizing legal aspects, incidence, and genetics of the resistance reported by studies published since 2009, focusing on farm animals and derived foods with the most global public health impact. Studies are related to poultry, cattle, and pigs, and mainly concentrate on non-typhoid Salmonella, Escherichia coli, and Staphylococcus aureus. We also describe legal aspects of antimicrobial use in this context; and the current occurrence of genetic elements associated with resistance to beta-lactams, colistin, and fluoroquinolones, among other antimicrobial agents. Data here presented may be useful to provide a better understanding of the Brazilian status on antimicrobial resistance related to farm animals and animal-derived food products.
“…reported in major poultry production countries from 2000 to 2020. Information obtained from: Brazil [ 41 , 121 – 129 ], Europe [ 130 – 132 ], USA [ 133 – 139 ], China [ 140 – 143 ] Fig. 3 Schematic representation of Gram-positive and Gram-negative antimicrobial resistance mechanisms.…”
Section: Poultry Production Systems and Amr Bacteriamentioning
confidence: 99%
“…reported in major poultry production countries from 2000 to 2020. Information obtained from: Brazil [ 41 , 121 – 129 ], Europe [ 130 – 132 ], USA [ 133 – 139 ], China [ 140 – 143 ]…”
Section: Poultry Production Systems and Amr Bacteriamentioning
confidence: 99%
“…reported in major poultry production countries from 2000 to 2020. Information obtained from: Brazil[41,[121][122][123][124][125][126][127][128][129], Europe[130][131][132], USA[133][134][135][136][137][138][139], China[140][141][142][143]…”
Economic losses and market constraints caused by bacterial diseases such as colibacillosis due to avian pathogenic Escherichia coli and necrotic enteritis due to Clostridium perfringens remain major problems for poultry producers, despite substantial efforts in prevention and control. Antibiotics have been used not only for the treatment and prevention of such diseases, but also for growth promotion. Consequently, these practices have been linked to the selection and spread of antimicrobial resistant bacteria which constitute a significant global threat to humans, animals, and the environment. To break down the antimicrobial resistance (AMR), poultry producers are restricting the antimicrobial use (AMU) while adopting the antibiotic-free (ABF) and organic production practices to satisfy consumers’ demands. However, it is not well understood how ABF and organic poultry production practices influence AMR profiles in the poultry gut microbiome. Various Gram-negative (Salmonella enterica serovars, Campylobacter jejuni/coli, E. coli) and Gram-positive (Enterococcus spp., Staphylococcus spp. and C. perfringens) bacteria harboring multiple AMR determinants have been reported in poultry including organically- and ABF-raised chickens. In this review, we discussed major poultry production systems (conventional, ABF and organic) and their impacts on AMR in some potential pathogenic Gram-negative and Gram-positive bacteria which could allow identifying issues and opportunities to develop efficient and safe production practices in controlling pathogens.
The current study was aimed to detect the existence of genes encode outer membrane proteins: transfer protein (traT) and increased serum survival (iss) which associated with resistance to complement bacterial lysis activity in different Enterobacteriaceae species isolates .Enterobacteriaceae isolates included in this study were (5) isolates of E. coli , K. pneumonia, Salmonella typhi and Shigella dysentery, (4) isolates of Proteus vulgaris and (2) isolates of Serratia marcescens which were isolated from different clinical infections. The traT and iss genes were revealed from whole DNA of 26 isolates of Enterobacteriaceae family, it was found that 21(80.7%) of Enterobacteriaceae isolates gave positive result for traT gene at 288bp while only 17(65.3%) of isolates gave positive result for iss gene at 258bp. It was observed that the traT gene was recognized among 100 % of Salmonella typhi and Serratia marcescens strains and the prevalence of a traT gene was showed among 80 % of K. pneumoniae and Shigella dysentery isolates whereas 75% of Proteus vulgaris and 60% of E. coli isolates contain the traT gene. The iss was found among 80% of both E. coli and Shigella dysentery isolates while it was observed in 60% of K. pneumonia and Salmonella typhi isolates and it was present only in 50% of Proteus vulgaris and Serratia marcescens isolates, as conclusion the study confirmed presence of traT and iss which linked to human complement resistance among local Enterobacteriaceae species and high rate of occurrence among extraintestinal isolates. .
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