Evaluation of the biofilm formation capacity of Pasteurella multocida strains isolated from cases of fowl cholera and swine lungs and its relationship with pathogenicity

Abstract

Pasteurella multocida is a Gram-negative bacillus that causes economic losses due to the development of respiratory diseases in several animal species. Among the mechanisms of virulence, the formation of biofilms is an important factor for bacterial survival in hostile environments. Studies of biofilm formation by P. multocida are needed because P. multocida is an important pathogen involved in respiratory infections. However, in contrast to other microorganisms, few studies of biofilm formation have examined P. multocida. Studies comparing the pathogenicity of microbial strains as a function of their biofilm production capacity are also rare. Consequently, the aim of this study was to evaluate the biofilm formation capacity of 94 P. multocida strains isolated from cases of fowl cholera and from swine lungs on polystyrene plates. The associations of the biofilm formation capacity with the pathogenicity index (PI) in vivo and with the presence of four genes (screened by PCR) of the tad locus (tadB, tadD, tadE and tadG), described as adhesion markers, were also determined. Strains from both animal origins were able to form biofilms. However, most of the specimens (52.13%) were classified as weak producers, and more than 40% of the strains of P. multocida (40.42%) did not produce biofilms. There was no significant difference (p>0.05) in the degree of biofilm production between the two sources of isolation. Of the analyzed strains, 56.52% contained all four genes (tadB, tadD, tadE and tadG). The PI arithmetic mean of the strains classified as non-biofilm producers was significantly different (p<0.05) from the PI of moderate-producer strains. The PI of specimens classified as weak biofilm producers also differed significantly (p<0.05) from that of the moderate-producer strains. The results indicate that even though the P. multocida strains isolated from cases of fowl cholera and swine lungs formed biofilms on polystyrene surfaces, adhesion was usually weak. The genes tadB, tadD, tadE and tadG were not significantly associated (p>0.05) with the production of biofilms and with the origin of a given strain. Finally, low virulence strains may suggest a higher biofilm formation capacity on polystyrene plates.

First report from Bangladesh on genetic diversity of multidrug-resistant Pasteurella multocida type B:2 in fowl cholera

“…Of the tested isolates, 36.37%, 27.27%, and 18.18% were SBF, MBF, and WBF, respectively (Figure -2a). The development of bacterial biofilms is presently recognized as one of the most relevant drivers of infections and one of the reasons for treatment failure with antibiotics [76][77][78]. In our present study, 18.18% of study strains were found as NBF (Figure -2a).…”

Isolation and identification of Escherichia coli, Salmonella and Pasteurella from holding grounds of live-bird markets at Addis Ababa, Ethiopia

“…Most bacteria pathogens of poultry especially E. coli, P. multocida and Staphylococcus gallinarum can be transmitted from one to others including humans in many ways and the most causal agents are excreted from chickens through excreta feces, nasal and oral secretion, and these pathogens can persist in the environmental litters, water, feed, holding and transporting cages, vehicles, clothing of animal attendants (Emery et al, 2017;Goldstone and Smith, 2016).…”

Down Regulation of Biofilm and Quorum Sensing Genes of Pseudomonas aeruginosa and Pasteurella multocida Isolated from Broiler Chicken Pericarditis Lesions by the Action of Some Essential Oils

“…Enzymatic lysis procedure of QIA amp RNeasy Mini kit (Qiagen, Germany, GmbH) used for RNA extraction according to (Yuan et al 2006). PCR reaction was applied in a Stratagene MX3005P real-time PCR machine where the specific primers (Table 1) for P. aeruginosa; 16s rDNA (housekeeping), pslA (biofilm), lasI (Quorum sensing) according to (Spilker et al 2004;Ghadaksaz et al 2015;Bratu et al 2006) respectively, while for P. multocida kmt1 (housekeeping) and tadG (Biofilm) according to (OIE 2012;de Emery et al 2017) while luxS (Quorum sensing) designed in this study, in brief preparation of PCR mix according to quantitect sybr green PCR kit as the following : 2x QuantiTect SYBR Green PCR Master Mix 12.5μl, Reverse transcriptase 0.25μl, each primers 0. 5μl, RNase Free Water 8.25μl, Template RNA 3μl.…”