This study was designed to isolate and characterize the pathogenicity of the most common bacteria causing enteric diseases in broilers in some Egyptian governorates. Enteric bacterial organisms like Escherichia coli (E. coli), Salmonellae, and Clostridia perfringens (C. perfringens) were isolated and identified from 100 dead and diseased broilers in Giza, El-Kalubia and El-Sharqia governorates. The samples were subjected to conventional isolation as well as biochemical and serological identification techniques. Molecular and toxigenic detection of C. perfringens were performed on one selected isolate by multiplex PCR. The pathogenicity test of some isolated bacterial strains was done on 80 a-day-old broiler chicks. The clinical observation, performance parameters, bacterial re-isolation and histopathological examination were carried out after bacterial challenge. The results revealed isolation of E. coli, Salmonella and C. perfringens in rates of 17, 11 and 39%, respectively. Serological identification of E. coli revealed that O78 (35.2%) and O1 (23.5%) were the highest isolated serotypes, while O117 (17.6%), O91 (11.7%), O112, and O146 (5.8% each) were the lowest ones. Salmonella enteritidis (S. enteritidis) was the most prevalent (36.3%) followed by S. kentucky, S. larochelle (27.2%, each) and S. inganda (9%). Multiplex PCR of C. perfringens strains revealed presence of both cpa and cpb genes that encoding to alpha (α) and beta (β) lethal toxins, respectively. The pathogenicity of E. coli O78, S. enteritidis, and C. perfringens were tested in broiler chickens. The results showed that E. coli O78, S. enteritidis and C. perfringens were pathogenic strains. It was concluded that enteric bacterial pathogens especially E. coli O78, S. enteritidis, and C. perfringens type C are widely distributed in Egyptian broiler chicken flocks and still causing severe losses because of mortalities and decreasing in production.