Intercorrência entre leucose enzoótica e brucelose em búfalos (Bubalus bubalis) em sistema de produção extensivo

Abstract

Pesq. Vet. Bras. 32(2):131-134, fevereiro 2012 131 RESUMO.-Objetivou-se com este estudo avaliar a intercorrência entre leucose enzoótica e brucelose em búfalos (Bubalus bubalis) em sistema de produção extensivo, pelo estabelecimento da prevalência de bubalinos reagentes às provas diagnósticas especíϐicas. Foram analisados sorologicamente 232 animais, pela técnica de imunodifusão dupla em gel de agarose para diagnóstico de leucose enzoótica e o teste do antígeno acidiϐicado tamponado, seguida pelos testes conϐirmatórios 2-mercaptoetanol e soroaglutinação lenta em tubos para diagnóstico de brucelose. As prevalências de búfalos que apresentaram positividade para leucose e brucelose foram 4,21% (10/232) e 5,18% (12/232), respectivamente. Os resultados obtidos nesta pesquisa permitiram concluir que as infecções estudadas encontram-se presentes na população avaliada, entretanto, com baixa prevalência e sem dependência de ocorrên-cia entre ambas.Intercorrência entre leucose enzoótica e brucelose em búfalos (Bubalus bubalis) em sistema de produção extensivo 1 Nancyleni P. Chaves 2* , Danilo C. Bezerra 2 , Larissa S. dos Santos 2 , Janaira S. This study was carried out to evaluate the intercurrence of brucellosis and enzootic leucosis in buffaloes in extensive production system by establishing the prevalence of reagent buffaloes for speciϐic diagnostic tests. We analyzed 232 animals serologically by the technique of double immunodiffusion in agarose gel (AGID) for diagnostic of leucosis and the tampon acidiϐied antigen (TAA) test, followed by conϐirmatory tests of 2-mercaptoethanol (2-ME) and slow serum agglutination (SSA) in tubes for diagnostic of brucellosis. The prevalence of buffaloes that were positive for leucosis and brucellosis tests were 4.21% (10/232) and 5.18%(12/232) respectively. The results of this investigation indicated that the diseases occur in the population studied, however, with low prevalence and without depending on the occurrence of both.INDEX TERMS: Brucella abortus, VLEB, bubalines , Maranhão.

Detecção de Brucella abortus em linfonodos de búfalas (Bubalus bubalis) em diferentes fases da gestação

“…O diagnóstico utilizado para brucelose em bubalinos tem sido baseado nas pesquisas de anticorpos por métodos sorológicos (Mathias et al 1998, Fosgate et al 2002, Molnár et al 2002, Nasir et al 2004, Bastianetto et al 2005, Chaves et al 2012, Silva et al 2014, Pereira et al 2015. Para demonstrar a presença desta bacteria a reação em cadeira da polimerase em tempo real (qPCR) detecta o DNA de Brucella spp.…”

Occurrence of anti-Brucella abortus and anti-Leptospira spp. antibodies in buffaloes from Paraíba state, Northeastern Brazil

“…Borriello et al (2006) reported a potential risk for humans after the consumption of milk and dairy products from buffaloes infected with Brucella abortus. Chaves et al (2012) in Maranhão and Calderón et al (2010) in Colombia reported the prevalence of brucellosis in buffaloes as 5.18% and 3%, respectively. The low frequency (1.5%) of brucellosis that was found in this study may be due to the number of bovine brucellosis control actions that are conducted by the animal defense service of Paraíba, including the inspection of vaccinations and animal movement control.…”

“…The second most common serotype was Pomona, whose maintenance host is the pig (FAINE et al, 1999), which also suggests contact with these species in breeding consortiums. The Canicola serotype, which was named the third most frequent, is usually associated with dogs as a maintenance host (FAINE et al, 1999).…”

“…In Brazil, in recent years, the frequency of seropositivity for buffalo leptospirosis has been reported to range from 37.7% to 80% (LANGONI et al, 1999;VIANA et al, 2009); for brucellosis, this variation was from 5.18% to 37.5% (BASTIANETTO et al, 2005;CHAVES et al, 2012). In the absence of studies on buffalo leptospirosis and brucellosis in the State of Paraíba, this study aimed to determine the occurrence of anti-Leptospira spp.…”

“…The main consequence of systemic infection with leptospirosis is abortion. The abortions usually occur in the third trimester of gestation and cause fetal death, with or without placental degeneration, which is followed by removal of the fetus weeks after infection (FAINE et al, 1999).…”