Anaplasma marginale infection in cattle from south-western Amazonia

Brito, L. G.; Oliveira, M. C. de S.; Rocha, Rodrigo Barros; Netto, F. G. da Silva; Marim, A. D.; Souza, G. C. R. de; Vendrame, Fabiano Benitez; Moura, Maria Manuela da Fonseca

doi:10.1590/s0100-736x2010000300011

Cited by 10 publications

(10 citation statements)

References 26 publications

(19 reference statements)

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“…This was similar to what was found by Torioni de Echaide et al (1998) in Oregon, USA, with 64% of positive animals, and lower than what was observed by Brito et al (2010) in Rondônia and Acre, Brazil, who found that 98.6% and 92.4% of the animals were positive, respectively, among animals aged between 4 and 12 months, using PCR in both studies. These higher results obtained by Brito et al (2010) may have been influenced by several factors, including age, since their study was conducted exclusively using calves. In the results from the present study, the calves also showed higher values (P < 0.01).…”

Section: Discussionsupporting

confidence: 90%

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Amorim

Wenceslau

Carvalho

et al. 2014

Rev. Bras. Parasitol. Vet.

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Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, BrazilComplexo tristeza parasitária bovina: diagnóstico e avaliação dos fatores de risco na Bahia, Brasil AbstractDirect diagnoses were made by using -blood smears and nested PCR (nPCR) tests on 309 blood samples from crossbred dairy cattle in the municipality of Ibicaraí, Bahia. From diagnostic blood smear slides, the observed parasitic frequencies were 31.1% for Anaplasma marginale and 20.4% for Babesia sp. From nPCR diagnoses, they were 63% for A. marginale, 34% for Babesia bigemina and 20.4% for Babesia bovis. There were significant differences (P < 0.01) between the two diagnostic methods (nPCR and blood smear slides). The compliance obtained from the kappa test was 0.41 and 0.48 for A. marginale and Babesia sp., respectively. The tick samples from the six farms analyzed using nPCR were only positive for A. marginale. Evaluation of the risk factors relating to the presence of ticks and the age of the animals showed that there was a significant association (P < 0.01) with the frequency of animals infected with both pathogens. Therefore, under the conditions studied, nPCR proved to be a good tool for diagnosing the agents of the bovine babesiosis and anaplasmosis complex because of its sensitivity and specificity in comparison with blood smears. The municipality of Ibicaraí is an area with endemic prevalence of bovine babesiosis and anaplasmosis confirmed by nPCR and A. marginale is the main agent of the disease.Keywords: Bovine Babesiosis and Anaplasmosis Complex, blood smear, nPCR, risk factors. ResumoRealizou-se o diagnóstico direto por esfregaço sanguíneo e nested PCR (nPCR) em 309 amostras de sangue de bovinos mestiços leiteiros provenientes do município de Ibicaraí, Bahia. A frequência observada no diagnóstico por lâminas de esfregaço sanguíneo foi 31,1% para Anaplasma marginale e 20,4% para Babesia sp. Enquanto que no diagnóstico por nPCR foi 63% para A. marginale, 34% para Babesia bigemina e 20,4% Babesia bovis. Verificaram-se diferenças significativas (P<0,01) na comparação entre os dois métodos de diagnósticos (nPCR e esfregaço sanguíneo). A concordância ao teste KAPPA obtida foi de 0,41 e 0,48 para A. marginale e Babesia sp., respectivamente. As amostras de carrapatos das seis propriedades analisadas por nPCR foram positivas apenas para A. marginale. Na avaliação dos fatores de risco verificou-se que a presença de carrapato e idade dos animais apresentaram associação significativa (P<0,01) com a frequência de animais infectados por ambos os patógenos analisados por nPCR. Portanto, nas condições estudadas, a nPCR revelou-se uma boa ferramenta para diagnóstico dos agentes do complexo tristeza parasitária bovina (TPB) devido a sensibilidade e especificidade, quando comparado ao esfregaço sanguíneo. O município de Ibicaraí

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Section: Discussionsupporting

confidence: 90%

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Amorim

Wenceslau

Carvalho

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…PCR based assay for sensitive diagnosis of B. babesiosis has been attempted by many workers with high degree of sensitivity and specificity worldwide (Fahrimal et al 1992;Salem 1998;Smeenk et al 2000;Santana et al 2001;Linhares et al 2002;Silva et al 2009;Brito et al 2010;Shams et al 2013). A PCR based assay for sensitive detection of B. bigemina was originally described by Figueroa et al (1992), targeting 278 bp fragment specific of parasite DNA.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Babesia bigemina infection in apparently healthy cattle of central plain zone of Punjab

Bhat

Singh

et al. 2014

J Parasit Dis

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Bovine babesiosis is an important tick-borne disease caused by the parasites belonging to the genus Babesia, distributed worldwide and infecting a wide range of domesticated and wild cattle, occasionally man. The present study was conducted to determine the prevalence of B. bigemina infection in apparently healthy cattle from central plain zone of Punjab, India. Examination of Giemsa-stained peripheral thin blood smears revealed 2.45 % (5/204) animals to be positive for piroplasms of B. bigemina. However, genomic DNA isolated from these blood samples when subjected to primary PCR revealed a positivity of 7.35 % (15/204) as detected by the amplification of a 278-bp product in the agarose gel. PCR products obtained from the primary PCR of B. bigemina, when employed as template in nested PCR produced the amplicons of desired size (170 bp) was detected in 30.39 % (62/ 204) of the samples. It can thus be concluded that B. bigemina infection is prevalent in apparently healthy cattle population of this region and PCR assays can serve as a valuable tool for epidemiological studies in endemic areas.

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“…The geographical distribution and clinical significance of Ehrlichia/Anaplasma infections in cattle are well documented for some species (E. ruminantum, A. marginale, and A. phagocytophilum) (Brito et al, 2010;Aktas and Özübek, 2015). However, little information is available on other species (e.g., E. minasensis).…”

Section: Anaplasmataceaementioning

confidence: 99%

“…However, little information is available on other species (e.g., E. minasensis). In Brazil, only A. marginale (Brito et al, 2010) and recently Ehrlichia sp. infections have been reported in cattle (Aguiar et al, 2014).…”

Section: Anaplasmataceaementioning

confidence: 99%

Molecular identification of Ehrlichia sp. in a beef cattle from brazilian Cerrado - case report

Oliveira

Silveira

Sorgatto

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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Ehrlichia infections in cattle are frequent in Africa but have also been reported in Brazil and North America. This paper reports natural infection by Ehrlichia sp. associated with Babesia bigemina and Anaplasma marginale in a calf in the municipality of Campo Grande, state of Mato Grosso do Sul, Brazil, presenting polioencephalomalacia. The molecular evidence, based on a fragment of the dsb gene, indicates a species of Ehrlichia genetically related to Ehrlichia canis and other species of the genus found in the tick Rhipicephalus (Boophilus) microplus and a calf from Brazil (99 to 100% identity). It was not possible to associate the clinical signs with Ehrlichia infection due to co-infections and histological evidence of another disease. However, the circulation of the bacteria in bovines in Brazilian Cerrado was confirmed and more attention should be given to clinical suspicion of tick-borne pathogens in cattle to clarify the pathogenic potential of Ehrlichia sp. RESUMO Infecções porEhrlichia em bovinos são frequentes na África, mas também foram relatadas no Brasil e na América do Norte. Este artigo relata uma infecção natural por Ehrlichia sp. associado a Babesia bigemina e Anaplasma marginale em um bezerro, no município de Campo Grande, Mato Grosso do Sul, Brasil, o qual apresentava polioencefalomalácia. A evidência molecular, baseada em um fragmento do gene dsb, indica uma espécie de Ehrlichia geneticamente relacionada a Ehrlichia canis e outras espécies do gênero encontradas no carrapato Rhipicephalus (Boophilus) microplus e em um bezerro do Brasil (99 a 100% de identidade). Não foi possível associar os sinais clínicos à infecção por Ehrlichia devido a coinfecções e evidências histológicas de outra doença. No entanto, a circulação da bactéria em bovinos no Cerrado brasileiro foi confirmada, e mais atenção deve ser dada à suspeita clínica de patógenos transmitidos por carrapatos em bovinos para esclarecer o potencial patogênico de Ehrlichia sp. Palavras-chave: patógenos transmitidos por carrapatos, gado, Ehrlichia, sinais neurológicos Recebido em 28 de setembro de 2018 Aceito em 28 de dezembro de 2018

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Anaplasma marginale infection in cattle from south-western Amazonia

Cited by 10 publications

References 26 publications

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Molecular detection of Babesia bigemina infection in apparently healthy cattle of central plain zone of Punjab

Molecular identification of Ehrlichia sp. in a beef cattle from brazilian Cerrado - case report

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