Avaliação de técnicas de biópsia hepática em ovinos

Néspoli, Pedro Brandini; Gheller, Valentim Arabicano; Peixoto, Paulo Vargas; França, Ticiana N.; Carvalho, A.Ú.; Araújo, Douglas K. Godoy de; Malm, Christina

doi:10.1590/s0100-736x2010000100005

Cited by 7 publications

(5 citation statements)

References 16 publications

(21 reference statements)

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“…Os ovinos, que apresentaram sinais clínicos, foram imediatamente retirados do experimento para evitar o agravamento da intoxicação e submetidos à biópsia hepática (Santos Júnior 2008, Néspoli et al 2010) ou necropsiados quando morriam. Ao final do experimento, nos animais que não adoeceram também foram realizadas biopsias hepáticas.…”

Section: Methodsunclassified

Avaliação da exposição solar na intoxicação experimental por Brachiaria decumbens em ovinos

et al. 2013

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ABSTRACT.-Porto M.R., Saturnino K.C., Lima E.M.M., Lee S.T., Lemos R.A.A., Marcolongo--Pereira C., Riet-Correa F. & Castro M.B. 2013 Twenty-six five-month-old lambs originated from flocks with no previous contact with Brachiaria spp. pastures were divided into three groups. Two groups (GS and GSB) were fed daily with fresh harvested Brachiaria decumbens ad libitum. GS was kept in an area with solar exposure and GSB was kept in stalls sheltered from solar exposure. Control group (GC) was also kept under solar exposure, but fed with Cynodon dactylon grass hay and Pennisetum purpureum fresh grass. All sheep from the three groups were supplemented with 200g daily of a commercial concentrated food. Evaluation of clinical signs was carried out daily and blood samples were collected twice a week to determine AST and GGT serum activities. Three out of nine lambs of GS presented clinical signs of Brachiaria spp. poisoning, and two died. One animal showed clinical signs and died in GSB. The main clinical signs observed were apathy, weight loss, photophobia, conjunctivitis, ocular mucous discharge and jaundice. Dermatitis due to photosensitization was not observed. Mean serum AST and GGT activities were significantly higher (p≤0,05) in the group exposed to sun, than in the other two groups, and the GGT activities were significantly higher in the group sheltered than in the control group (p≤0,05). All animals at the end of the experiment were submitted to liver biopsy and died lambs were necropsied. Histopathological evaluation of liver samples from sheep with clinical signs evidenced swelling and vacuolization of hepatocytes, individual hepatocytes necrosis, presence of foamy macrophages, crystal negative images within bile ducts and foamy macrophages, biliary duct hyperplasia and periportal mononuclear infiltration. These results suggest that sun exposure does not define intoxication, but exacerbates the toxicity of the grass. RESUMO.-Foram utilizados 26 ovinos, entre 3 e 4 meses de idade, divididos em 3 grupos, provenientes de rebanhos que nunca tiveram contato com pastos de Brachiaria spp.. Dois grupos receberam Brachiaria decumbens no cocho diariamente ad libitum, sendo que um deles permaneceu em área com exposição solar (GS) e o outro foi mantido em baias cobertas protegidos do sol (GSB). O grupo controle (GC) foi também mantido em local com exposição solar e alimentado com feno de Cynodon dactylon e capim Pennisetum purpureum triturado. Todos os grupos receberam alimentação em cochos e foram suplementados com 200g/ dia/animal de ração comercial para ovinos. Foi realizada a avaliação clínica diária dos ovinos e colhidas amostras duas vezes por semana para dosagem sérica de AST e GGT. Os

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Section: Methodsunclassified

Avaliação da exposição solar na intoxicação experimental por Brachiaria decumbens em ovinos

et al. 2013

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“…Recently [ 14 ], it was demonstrated that the exclusive consumption of Malachra fasciata also caused signs of photosensitization in sheep, seven days after the beginning of ingestion of the plant. The photosensitizing effect of F. humboldtiana was reproduced in sheep and cattle, respectively, 10 and 3 days after the animals were introduced to pastures invaded by the plant [ 8 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…All sheep underwent histologic evaluation of the liver using ultrasound-guided biopsies with a number 14 catheter on D0, using a previously reported technique [ 11 ]. The animals were kept stationary, and the catheter was inserted into the 11th intercostal space, after local trichotomy (10 cm 2 ), surgical antisepsis and local blockade, using an anesthetic button, with 5 mL of 2% lidocaine hydrochloride (Dorfin ® , Ceva Animal Hearth, Paulinia, Brazil), in the intercostal muscle.…”

Section: Methodsmentioning

confidence: 99%

Primary Photosensitization by Chamaecrista serpens in Santa Inês Sheep

Mendonça

Caymmi

Silva

et al. 2022

Animals

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This study aimed to clarify the type of photosensitization induced by C. serpens and to verify if the plant remains toxic after being collected and stored. Eight crossbred sheep, aged between 6 and 36 months, were divided into three groups (G1 to G3). Over 30 days, daily, G1 received an exclusive diet of C. serpens, and G2 and G3 received 10 g/kg/BW and 20 g/kg/BW, respectively. Two other sheep were used as controls (CG). Before administration, the plant had been harvested every 15 days. Liver biopsies and blood samples were taken from all sheep on day zero and weekly. All sheep that received the plant developed clinical signs of photosensitization, and no changes were observed in the serum activities of AST and GGT. On day 30, all sheep except Ov1 from G1 and Ov7 were euthanized and necropsied. All sheep that received the plant developed clinical signs. Macroscopic or histologic lesions were not observed in the liver. Ov 1 recovered 13 days after the end of ingestion. These results demonstrated that C. serpens causes primary photosensitization. It is advisable to avoid grazing on pastures invaded by the plant or to remove them from the pastures immediately after observing the first signs.

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“…Nos caprinos que não vieram à morte em 48 horas após a primeira administração foi realizada a coleta de fragmentos do fígado por meio de biópsia hepática percutânea guiada por ultrassonografia conforme descrito por Néspoli et al (2010). Nos animais que receberam as folhas e sobreviveram, foi realizada a biopsia hepática 15 dias após o último procedimento.…”

Section: Methodsunclassified

Comparação dos efeitos das folhas de Cestrum axillare Vell. com as saponinas isoladas em caprinos

et al. 2018

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ABSTRACT.-Marinho J.B.R., Carvalho A.U., Pierezan F., Keller K.M., Riet-Correa F. Center-West regions and in coastal areas of the Northeast Brazil. Spontaneous poisoning was described in cattle, goats and sheep, with clinical signs evidenced within 24 hours after ingestion of the leaves and death within 48 hours after signs onset. The clinical signs observed in acute poisoning are apathy, anorexia, ruminal arrest, arched back, constipation with feces in small spheres, sometimes covered with mucus and blood streaks, muscle tremors, staggering gait and sometimes sialorrhoea. Neurological signs may be observed, due to interference in the urea cycle due to hepatic insufficiency resulting in hyperammonemia (hepatic encephalopathy). The main pathological finding is centrilobular hepatic necrosis. The toxic principle present in C. axillare was not yet definitively proven, but some authors attribute the toxicity of the plant to the presence of saponins gitogenin and digitogenin. However, it has not been determined whether the saponins present in C. axillare are responsible for the hepatotoxic effect of the plant. Thus, the objective of this work is to determine if the saponins are the compounds responsible for the hepatotoxic effects produced by the ingestion of the leaves of C axillare, using goats as experimental model. For this, the effects of the administration of the leaves were compared with those produced by the saponins isolated from the leaves in goats. Six goats were randomly assigned to three experimental groups that received [1] dry leaves of C. axillare (animals A1 and A2), [2] saponins extract from leaves (animals S1 and S2) or [3] control group (animals C1 and C2). For goats receiving the dry leaves the administered dose of plant was 10g/kg for one animal (A1) and 5g/kg for the other one (A2). For animals receiving the saponins extract, administration was done at a dose equivalent to 20g/kg repeated after 24 hours. The dry leaves administered at a dose of 10g/kg to a goat produced toxic effects, with alterations in biochemistry (indicating hepatic lesion) and histopathology showing centrilobular hepatic necrosis. At the dose of 5 g/kg of dry leaves, clinical signs of poisoning were not observed, but hepatic necrosis was found; after 15 days after the last administration, the hepatic parenchyma of this animal was already normal, with only hemorrhagic areas, demonstrating full regeneration. The administration of extracts of saponins containing gitogenin and digitogenin to goats did not produce significant toxic effects, proving that these compounds are not responsible for intoxication. In addition, goats are a good experimental model for studies of this intoxication.

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Avaliação de técnicas de biópsia hepática em ovinos

Cited by 7 publications

References 16 publications

Avaliação da exposição solar na intoxicação experimental por Brachiaria decumbens em ovinos

Avaliação da exposição solar na intoxicação experimental por Brachiaria decumbens em ovinos

Primary Photosensitization by Chamaecrista serpens in Santa Inês Sheep

Comparação dos efeitos das folhas de Cestrum axillare Vell. com as saponinas isoladas em caprinos

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