Isoenzimas no monitoramento da deterioração de sementes de Euterpe espiritosantensis Fernandes

Martins, Cibele Chalita; Nakagawa, João; Ramos, Paulo Roberto Rodrigues

doi:10.1590/s0100-67622011000100010

Cited by 4 publications

(5 citation statements)

References 12 publications

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“…The results of this study reinforced that genetic differences among seeds from different sites, associated or not with climatic conditions, influenced morphological, physiological and biochemical characteristics of seeds in this species (Martins, Bovi, Mori, & Nakagawa, 2007;Martins et al, 2011).…”

Section: Resultssupporting

confidence: 73%

Isoenzyme activity in monitoring deterioration of Balfourodendron riedelianum seeds

et al. 2018

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In this study, we sought to identify efficient enzymes for use in monitoring the deterioration and loss of germination of Balfourodendron riedelianum (Engler) Engler seeds. The research was conducted at the Laboratório de Tecnologia de Sementes e Mudas of the Universidade Estadual do Oeste do Paraná, Campus Marechal Cândido Rondon, Paraná State, Brazil. Seeds were harvested at three collection sites and later submitted for processing, drying, initial characterization and storage in airtight packaging under controlled and uncontrolled storage conditions and uncontrolled. Tests were conducted at 0, 120, 240, and 360 days using a germination test, germination speed index, accelerated ageing test and the activity of peroxidase, phenylalanine ammonia-lyase and β-1,3-glucanase. The experiment was a completely randomized design. The results were subjected to regression analysis at 5% probability. Ageing was different among sample sites and between different storage conditions. The decrease in viability of seeds of Balfourodendron riedelianum (Engler) Engler was detected based on the activity of peroxidase enzymes in seeds from the Diamante do Oeste and Missal sites and the enzyme β-1,3-glucanase in seeds from the Missal site after storage periods of 0, 120, and 240 days.

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Section: Resultssupporting

confidence: 73%

Isoenzyme activity in monitoring deterioration of Balfourodendron riedelianum seeds

et al. 2018

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“…Possivelmente, o teor de água das sementes seja suficiente para manter a rota funcionando para atender às necessidades específicas, como a produção de produtos secundários. Martins et al (2011) também concluíram que essa enzima não foi eficiente na identificação dos graus de deterioração de sementes de Euterpe espiritosantensis.…”

Section: Resultsunclassified

ALTERAÇÕES FISIOLÓGICAS E ATIVIDADE ENZIMÁTICA EM SEMENTES ARMAZENADAS DE Melanoxylon brauna Schott.

Borges

Flores

Ataíde

et al. 2015

CERNE

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ALTERAÇÕES FISIOLÓGICAS E ATIVIDADE ENZIMÁTICA EM SEMENTES ARMAZENADAS DE Melanoxylon brauna Schott.RESuMO: A temperatura e a umidade relativa estabelecem as condições fundamentais na conservação de sementes, quer sejam recalcitrantes, quer sejam ortodoxas. A conservação de sementes de braúna sob umidades relativas de 55, 75 e 93% e temperatura de 20ºC foi realizada com os objetivos de avaliar as respostas da germinação e do vigor, relacionandoas à permeabilidade da membrana, atividade de enzimas, teor e peroxidação de lipídios. A qualidade das sementes decresce com o aumento do teor de água do ambiente, com decréscimo também das atividades das enzimas superóxido dismutase e catalase e aumento da atividade da enzima glicose-6-fosfato desidrogenase. A permeabilidade das membranas é ampliada com o aumento da deterioração das sementes. Não há relação entre as alterações no teor e peroxidação de lipídios e a deterioração das sementes. PHYSIOLOGICAL AND ENZYME ACTIVITY CHANGES IN STORED SEEDS OF Melanoxylon brauna Schott.AbSTRACT: Temperature and relative humidity set the basic conditions for the preservation of recalcitrant and orthodox seeds. The seed conservation of braúna under relative humidities of 55, 75 and 93% at 20°C was performed with the aim of evaluating germination and vigor responses, relating them to the membrane permeability, enzyme activity, content and lipid peroxidation. Increasing environment water content decreases seed quality and also decreases the activity of the enzymes superoxide dismutase and catalase, and increases activity of glucose-6-phosphate dehydrogenase. The membrane permeability increases with increasing deterioration of the seeds. There is no relationship between the changes in lipids content and peroxidation and seed deterioration.

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“…The membrane systems of seeds harvested at 45% water content had a greater effect of exposure to oxygen due to the higher content of mechanical damage that they suffered during the process of husking, resulting in a lower expression of peroxidase enzyme, which can be also proven by the results of physiological tests where their poor performance is noted in both hybrids studied (Martins et al, 2011).…”

Section: Resultsmentioning

confidence: 88%

“…The balance between the generation and removal of radicals during drying and storage of seeds, relates to its longevity (Martins et al, 2011), suggesting that the difference observed between the seeds harvested with different water content is related to the seeds age. (1 -45% T, 2 -40% T, 3 -35% T, 4 -45% UT, 5 -40% UT and 6 -35% UT); Hybrid 2 -BM 3061 (7 -45% T, 8 -40% T, 9 -35% T, 10 -45% UT, 11 -40% UT and 12 -35% UT), disclosed for peroxidase.…”

Section: Resultsmentioning

confidence: 99%

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Isoenzyme activity in maize hybrid seeds harvested with different moisture contents and treated

Ferreira¹,

Ferreira²,

Oliveira³

et al. 2015

J. Seed Sci.

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-The analysis of isoenzyme activity is an important monitoring and characterization tool of the physiological quality of seeds and to understand the deterioration. The purpose of this work was to study the isoenzyme expression allied to the quality of maize hybrid seeds harvested at different moisture levels and subjected to chemical treatment. A completely randomized experimental design was used with four replicates, in a 3x2 factorial arrangement with three moisture levels (45%, 40% and 35%), and two forms of seeds tillage (with and without treatment). Seeds from maize hybrids, semi-hard BM 810 and dented BM 3061, were used. Seeds were manually gathered on ears. Chemical treatment was performed with commercial products Maxin ® + K-obiol ® + Actellic ® . Seed quality was assessed by moisture test, incidence of mechanicals damage, first count of germination, germination, emergence, emergence speed index, mean emergence time, accelerated aging, and electrical conductivity. Isoenzyme expressions were assessed by means of the systems superoxide dismutase (SOD), catalase (CAT), esterase (EST), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), peroxidase (PO) and α-amilase. Isoenzyme expressions are different, depending on moisture levels at harvest, the hybrid maize and seeds quality. Seeds treatment does not interfere in their isoenzymes expression.Index terms: Zea mays, isoenzyme expression, physiological quality.Atividade isoenzimática em sementes de milho híbridos colhidas com diferentes teores de água e tratadas RESUMO -A análise da atividade enzimática é uma importante ferramenta no monitoramento e caracterização da qualidade fisiológica de sementes e no entendimento da deterioração. Objetivou-se com este trabalho estudar a expressão de isoenzimas aliada a qualidade de sementes de milho híbrido colhidas com diferentes teores de água e submetidas ao tratamento químico. O delineamento utilizado foi o inteiramente casualizado, com quatro repetições, em esquema fatorial 3x2, sendo três teores de água (45%, 40% e 35%), e duas formas de manejo das sementes (tratadas e não tratadas). Foram utilizadas sementes de milho dos híbridos: BM 810, semiduro e BM 3061, dentado, colhidas manualmente em espigas. O tratamento químico foi realizado com os produtos comerciais Maxin ® + K-obiol ® + Actellic ® . A qualidade das sementes foi avaliada pelos testes de umidade, incidência de danos mecânicos, primeira contagem de germinação, germinação, emergência, índice de velocidade de emergência, tempo médio de emergência, envelhecimento acelerado e condutividade elétrica. As expressões isoenzimáticas foram avaliadas por meio dos sistemas superóxido dismutase (SOD), catalase (CAT), esterase (EST), álcool desidrogenase (ADH), malato desidrogenase (MDH), peroxidase (PO) e α-amilase. A expressão de isoenzimas é divergente dependendo do teor de água na colheita, do híbrido e da qualidade das sementes. O tratamento de sementes não interfere na expressão das isoenzimas.Termos para indexação: Zea mays, expressão isoenzimática...

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Isoenzimas no monitoramento da deterioração de sementes de Euterpe espiritosantensis Fernandes

Cited by 4 publications

References 12 publications

<b>Isoenzyme activity in monitoring deterioration of <i>Balfourodendron riedelianum</i> seeds

<b>Isoenzyme activity in monitoring deterioration of <i>Balfourodendron riedelianum</i> seeds

ALTERAÇÕES FISIOLÓGICAS E ATIVIDADE ENZIMÁTICA EM SEMENTES ARMAZENADAS DE Melanoxylon brauna Schott.

Isoenzyme activity in maize hybrid seeds harvested with different moisture contents and treated

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