Produção de biossurfactante por Aspergillus fumigatus utilizando resíduos agroindustriais como substrato

Castiglioni, Gabriel Luis; Bertolin, Telma Elita; Costa, Jorge Alberto Vieira

doi:10.1590/s0100-40422009000200005

Cited by 31 publications

(12 citation statements)

References 14 publications

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“…When compared with the results obtained in the current study, species from genus Aspergillus appear to be most promising for lipase production by SSF, since they produce high levels of the enzyme. Castiglioni et al (2009) tested lipase production by Aspergillus fumigatus under SSF by using shell and rice bran oil, and olive added as carbon source. The obtained value of the total activity of 117.02 U gds -1 was similar to that obtained in the present study by Aspergillus caespitosus URM 5938 with almond bran licuri and olive oil as carbon source and total activity of 117.33 U gds -1 .…”

Section: Effect Of Temperature On the Enzymatic Activity Stabilitymentioning

confidence: 99%

High-level lipase production by Aspergillus candidus URM 5611 under solid state fermentation (SSF) using waste from Siagrus coronata (Martius) Becari

Farias¹,

Souza²,

Sousa³

et al. 2015

Afr. J. Biotechnol.

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The current study evaluated lipase production by Aspergillus candidus URM 5611 through solid state fermentation (SSF) by using almond bran licuri as a new substrate. The microorganism produced high levels of the enzyme (395.105 U gds -1 ), thus surpassing those previously reported in the literature. The variable moisture content, the inductor concentration, temperature and pH were analyzed. The best production conditions were initial moisture content of 75%, 0% of inductor, at 25°C, pH 5.5, in 10.0 g of substrate. The variables initial moisture and inductor contents showed significant effects on lipase production by increasing enzyme activity in about 200% when compared with the initial screening (197.44 U gds -1 ). Optimum lipase activity was obtained at pH 2.5 at an optimum temperature of 65°C. The lipase was stable from pH 2.5 to 9.0 and at temperatures between 30 to 65°C. Results from the study are promising for the economic use and value addition from these important agro residues, which are abundantly available in Brazil. This is the first report on lipase production by A. candidus URM 5611 reaching a much higher yield of enzyme under SSF with almond bran licuri used as substrate.

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Section: Effect Of Temperature On the Enzymatic Activity Stabilitymentioning

confidence: 99%

High-level lipase production by Aspergillus candidus URM 5611 under solid state fermentation (SSF) using waste from Siagrus coronata (Martius) Becari

Farias¹,

Souza²,

Sousa³

et al. 2015

Afr. J. Biotechnol.

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“…It only needs a low percentage of free water to be conducted and it uses low cost substrates such as agricultural wastes, which act both as a source of nutrients for the fermentation process (Martins et al, 2008;Messias et al, 2011;Pandey, 2003), Martins et al, 2008). Among these microorganisms, filamentous fungi are considered the most suitable for processes involving SSF due to their ability to grow with a low amount of free water (Castiglioni et al, 2009;Thomas et al, 2013;Vendruscolo et al, 2007) together with their efficiency to degrade some pollutants (Rigas et al, 2007;Ye et al, 2011). Particularly, the production of lipolytic enzymes by filamentous fungi in SSF from agro-industrial wastes has attracted great interest, since these enzymes can be easily extracted from the fermentation medium as they are mostly extracellular (Moura et al, 2013;Roveda et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Costa

Hermann

García-Román

et al. 2017

Braz. J. Chem. Eng.

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-Filamentous fungi can easily degrade agro-industrial wastes in solid-state fermentation processes, synthesizing many important commercial biocompounds, such as lipolytic enzymes. The aim of this study was to evaluate the effect of the composition of the solid culture medium on the production of lipolytic enzymes by the fungus Aspergillus niger. Rice, wheat and soybean bran were mixed to prepare the culture medium, which was supplemented with glucose, glycerol or soybean oil. Four mixture experimental designs were used to find the best medium for enzyme production. According to our results, the highest lipolytic activity values were achieved with a mixture of rice bran and glycerol (19.844 U·g -1 ) or with rice bran only (13.267 U·g -1 ). Thus, the lipolytic enzyme could be produced without any additional carbon source apart from rice bran, although glycerol addition induced a higher production.

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“…Por sua vez, dentre os de alto peso molecular estão os polissacarídeos, as proteínas, as lipoproteínas e os lipopolissacarídios (Pacwa-Plociniczak et al, 2011). Muitos microrganismos são capazes de utilizar diferentes substratos como fonte de alimento, sendo extensa a lista de substratos usados para produção de biossurfactantes, com destaque para hidrocarbonetos de petróleo (Mahjoubi et al, 2013), óleo vegetal residual (Rufino et al, 2014), resíduos agroindustriais (Castiglioni et al, 2009;Fontes et al, 2012), carboidratos (Viramontes-Ramos et al, 2010), dentre outros. A produção de biossurfactantes a partir de substratos renováveis tem ganhado atenção considerável nos últimos anos (Marti et al, 2015).…”

Section: Introductionunclassified

Produção De Biossurfactantes Por Cepas Bacterianas De Origem Marinha Utilizando Querosene Como Fonte De Carbono

et al. 2017

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RESUMOEste trabalho teve como objetivo avaliar a capacidade de bactérias marinhas, isoladas de região portuária de Fortaleza, Ceará, em produzir compostos biossurfactantes utilizando querosene comercial como fonte de carbono. Foram utilizadas 22 cepas bacterianas, sendo 14 provenientes de amostras de água e 8 de sedimento. Os microrganismos foram crescidos por um período de 72 horas em meio de cultura Luria Bertani (LB) tendo como única fonte de carbono o querosene na concentração de 2%. Com o sobrenandante (líqui-do metabólico -LM) das culturas bacterianas foram realizados os seguintes testes de avaliação da produção de biossurfactantes: colapso da gota, atividade de emulsificação, capacidade de formação e estabilidade de emulsão. Foi avaliada a capacidade dos isolados em produzir hemólise em meio Ágar Sangue, indicativo da produção de biossurfacatntes. Do total de isolados, 36% obtiveram forte atividade no teste do colapso da gota, 50% produziram boas emulsões (acima de 40%), apresentando boa estabilidade até 168 horas de observação e 63% dos isolados produziram halos de hemólise. As cepas obtiveram bons resultados nos testes realizados, indicando um potencial em degradar querosene comercial, utilizando-o como fonte de carbono e produzindo compostos biossurfactantes/bioemulsificantes. Palavras-chave: Querosene; Surfactantes Biológicos; Degradação; Emulsão. ABSTRACTBiosurfactant production by marine bacterial strains using kerosene as a carbon source. The aim of this work was to evaluate the ability of marine bacteria isolated from harbor region of Fortaleza, Ceará, to produce biosurfactants compounds using kerosene as the carbon source. Twenty-two strains were used, of which 14 from water samples and 8 from sediment samples. The microorganisms were grown for 72 hours in Luria Bertani (LB) using kerosene 2% as the only carbon source (v/v). The ability of these strains to produce biosurfactant compounds was investigated using the extracted supernatant from bacterial cultures (metabolic liquid, ML) through the following tests: drop collapse test, emulsification activity, formation hability and emulsion stability. The isolated bacterias hability to produce hemolysis in Blood Agar medium, indicative of biosurfactant production, was evaluated. A total of 36% of strains showed strong activity in the drop collapse test, while about 50% of strains produced emulsions higher than 40%, showing good stability until 168 hours of observation and 63% produced hemolytic activity. The isolated strains showed good results in the performed tests indicating biotechnological potential to degrade kerosene and using it as a source of carbon and produce biosurfactant/bioemulsificants compounds.Keywords: Kerosene; Biological Surfactants; Degradation; Emulsion. INTRODUÇÃOOs surfactantes são compostos tensoativos com ampla utilização nos diferentes setores da indústria.Essas substâncias apresentam estrutura molecular com grupos hidrofílicos e hidrofóbicos e possuem a capacidade de reduzir a tensão superficial de misturas com fases e pola...

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Produção de biossurfactante por Aspergillus fumigatus utilizando resíduos agroindustriais como substrato

Cited by 31 publications

References 14 publications

High-level lipase production by Aspergillus candidus URM 5611 under solid state fermentation (SSF) using waste from Siagrus coronata (Martius) Becari

High-level lipase production by Aspergillus candidus URM 5611 under solid state fermentation (SSF) using waste from Siagrus coronata (Martius) Becari

LIPASE PRODUCTION BY Aspergillus niger GROWN IN DIFFERENT AGRO-INDUSTRIAL WASTES BY SOLID-STATE FERMENTATION

Produção De Biossurfactantes Por Cepas Bacterianas De Origem Marinha Utilizando Querosene Como Fonte De Carbono

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