Estratégias utilizadas no combate a resistência bacteriana

Silveira, Gustavo Pozza; Nome, Faruk; Gesser, José C.; Sá, Marcus M.; Terenzi, Hernán

doi:10.1590/s0100-40422006000400037

Cited by 78 publications

(62 citation statements)

References 44 publications

(72 reference statements)

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“…On the other hand, it cannot be unconsidered of other protection mechanisms are involved such as efflux pump, enzymatic modifications and modifications of target sites that can be resistant to microorganism metabolites (Garvey et al, 2011). The specific mechanisms involved in the antimicrobial action of terpenes remain poorly characterized (Helander et al, 1998;Silveira et al, 2006).…”

Section: Antimicrobial Activity (Model Agar Diffusion)mentioning

confidence: 99%

Myracrondruon urundeuva Allemo: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

Santos¹,

Lima²,

Andrade³

et al. 2017

Afr. J. Biotechnol.

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Caatinga biome features species with high therapeutic potential such as Aroeira do Sertão (Myracrodruon urundeuva Allemão) which has long history in folk medicine. This study aimed to chemically characterize the secondary metabolites of hydroethanolic extract and hexane, chloroform, acetate, hydroethanolic fractions from Myracrodruon urundeuva (Allemão) possibly responsible antioxidant and antimicrobial activity, besides to evaluate the inotropic effect of crude extract in the left atrium of Cavia porcellus. Phytochemical composition was evaluated by spectrophotometry and highperformance liquid chromatography (HPLC). Antioxidant activity was assessed by 2,2-diphenyl-1-picryl hidrazyl (DPPH•) scavenging and reactive substances to thiobarbituric acid methods. Inotropic effect was evaluated using the isolated organ model. Data were expressed as mean ± SEM and differences determined by ANOVA followed by Tukey test. Phytochemical screening and the HPLC confirmed the presence of flavonoids, terpenes, tannins. Acetate and Hydroethanolic extract showed inhibition of DPPH values of 81.56 and 93.28% respectively. On the other hand, TBARS reduced in extract were 64.13% in a concentration of 150 µg/mL. Hydroethanolic extract and acetate fraction with concentrations of 50 and 100 mg/mL showed inhibition zones between 11.00 and 20.50 mm suggesting antimicrobial activity against Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae bacteria and Candida albicans. The isolated atrium model demonstrated that the extract acts non-competitively antagonizing muscle calcium currents, decreasing the force of contraction by about 60%. Flavonoids, terpenes and tannins are responsible for M. urundeuva (Allemão) biological effects.

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Section: Antimicrobial Activity (Model Agar Diffusion)mentioning

confidence: 99%

Myracrondruon urundeuva Allemo: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

Santos¹,

Lima²,

Andrade³

et al. 2017

Afr. J. Biotechnol.

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“…Many bacteria have developed resistance to a large number of antimicrobial agents available on the market; these bacteria are considered challenges for medicine. Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE) are two examples of antimicrobialresistant microorganisms (Silveira et al, 2006;Wright, 2011). Compounding the problem of antimicrobial resistance, there are few new antimicrobial agents emerging in the market (Wright, 2011).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…It has been used successfully in treating some infections caused by resistant gram-positive bacteria, such as MRSA and VRE. Furthermore, resistance to daptomycin is rare worldwide (Silveira et al, 2006;Picazo et al, 2011; Brunton, Chabner, Knollman, 2012;Chitnis et al, 2013;Sader et al, 2014).The quality of medicines has always been a concern to the World Health Organization (WHO), because without the assurance that these products meet the standards of quality, safety, and efficacy, worldwide health services can be affected. In addition, governments allocate a substantial part of their health budget to purchasing medicines.…”

mentioning

confidence: 99%

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A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

Tótoli

Salgado

2015

Braz. J. Pharm. Sci.

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Daptomycin is the first approved drug from a new class of antimicrobials, the cyclic lipopeptides, and is a very important antimicrobial agent in current clinical practice. Currently, there are no "green" analytical methods described in the literature to analyze the typical pharmaceutical dosage form of daptomycin. Thus, the aim of this work was to validate an environment-friendly spectrophotometric method in the UV region, for the analysis of daptomycin as a lyophilized powder. Water was used as diluent and the analyses were carried out on a spectrophotometer at 221 nm. The method met all validation requirements of the ICH guidelines, over a concentration range of 6-21 µg mL -1 . A Student's t-test demonstrated that the proposed method was comparable to an HPLC method previously validated. Thus, the validated spectrophotometric method could quantify daptomycin in a powder form for injectable solutions, while being an economical, rapid, and "green" alternative for routine analysis in quality control.Uniterms: Antimicrobials/quality control. Daptomycin/determination. Spectrophotometry/UV region/ quantitative analysis. Spectrophotometry/green analytical method.A daptomicina é o primeiro membro aprovado de uma nova classe de antimicrobianos, os lipopeptídeos cíclicos, e é muito importante para a prática clínica atualmente. Não existem métodos analíticos "verdes" descritos na literatura para a análise da daptomicina na forma farmacêutica. Desta forma, o objetivo deste trabalho foi a validação de método espectrofotométrico na região do UV ambientalmente favorável para análise da daptomicina em pó liofilizado. A água foi escolhida como diluente e as análises foram realizadas em 221 nm. O método atendeu a todas as exigências de validação dos guias do ICH, na faixa de 6-21 µg mL -1 . Teste t de Student mostrou que o método proposto é intercambiável com método de HPLC previamente validado. Assim, o método espectrofotométrico validado é capaz de quantificar a daptomicina em pó para solução injetável e é uma opção econômica, rápida e "verde" para análises de rotina do controle de qualidade deste fármaco.Unitermos: Antimicrobianos/controle de qualidade. Daptomicina/determinação. Espectrofotometria/ região do UV/análise quantitativa. Espectrofotometria/método analítico verde. INTRODUCTIONIt is widely known that microbial resistance is a major problem worldwide. Many bacteria have developed resistance to a large number of antimicrobial agents available on the market; these bacteria are considered challenges for medicine. Methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE) are two examples of antimicrobialresistant microorganisms (Silveira et al., 2006;Wright, 2011). Compounding the problem of antimicrobial resistance, there are few new antimicrobial agents emerging in the market (Wright, 2011).One recent development in antimicrobial treatment is the introduction of daptomycin into the marketplace (Figure 1). This is the first antimicrobial agent from the cyclic lipopeptide class appro...

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“…The ethnopharmacological studies have cataloged lately a large number of plants used by the samepeople who got their medica l activities confirmed [4][5][6] . The growing interest in medicinal plant research in recent decades is due to an increasing apparition of adverse effects of conventional drugs and the increase of microbial resistance to antibiotics, the researchers are looking more carefully the natural products that could provide effective treatment for diseases and infections and a low toxicity to the patient 7,8 . Two main factors are crucial to microbial resistance: the emerging and re-emerging diseases and the resistance to antibiotics 9 .…”

mentioning

confidence: 99%

Evaluation of antibacterial, antifungal and modulatory activity of methanol and ethanol extracts of Padina sanctae-crucis

Nogueira¹,

Morais²,

Brito³

et al. 2014

Afr H. Sci.

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Background: Multi-resistant microorganisms such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida tropicalis e Candida krusei are the main causes of microbial infections. Padina sanctae-crucis is a seaweed often used to check the contamination of ecosystems by materials such as heavy metals, but studies of the antimicrobial activity of the same seaweed were not found. Methods:The tests for the minimum inhibitory concentration and modulation of microbial resistance, with the use of ethanolic and methanolic extracts of Padina Sanctae-cruces combined with drugs of the class of aminoglycosides and antifungal were used to evaluate the activity against the cited microorganisms. Results: Was observed a modulation of antibiotic activity between the natural products and the E. coli and S. aureus strains, indicating a synergism and antagonism respectively. Conclusions:The results showed a moderate modulatory effect against some microorganisms studied.

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Estratégias utilizadas no combate a resistência bacteriana

Cited by 78 publications

References 44 publications

Myracrondruon urundeuva Allemo: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

Myracrondruon urundeuva Allemo: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

Evaluation of antibacterial, antifungal and modulatory activity of methanol and ethanol extracts of Padina sanctae-crucis

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