Emprego de compostos organometálicos mononucleares de paládio(II) na ativação de macrófagos peritoneais de camundongos

Almeida, Eduardo Tonon de; Mauro, Antônio Eduardo; Santana, A. M.; Netto, Adelino Vieira de Godoy; Carlos, Iracilda Zeppone

doi:10.1590/s0100-40422005000300008

Cited by 17 publications

(6 citation statements)

References 11 publications

(11 reference statements)

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“…Recent research has demonstrated that organopalladated compounds containing diphenylphosphine ligands can activate macrophages in vitro (De Almeida et al, 2005) via immunological activation. This effect was demonstrated by assessing the amount of H 2 O 2 released in cultures of peritoneal macrophages from Swiss mice in the presence of organopalladated compounds of the type [Pd(dmba)(X)(dppp)] (dmba = N,N-dimethylbenzylamine; dppp = 1,3-bis(diphenylphosphine) propane; X = Cl, N 3 , NCO, NCS).…”

Section: Resultsmentioning

confidence: 99%

“…The organometallic complexes [Pd(dmba)(Cl) tu] and [Pd(dmba)(N 3 )tu] were synthesized according to the literature (Lucca Neto et al, 1998;De Almeida et al, 2005), at the Laboratory of Organometallic and Coordination Chemistry, Department of General and Inorganic Chemistry, UNESP, Araraquara, Brazil. Cisplatin (FH Fauld-ing & Co. Ltd., Adelaide, Australia) was used as standard drug.…”

Section: Organometallic Complexesmentioning

confidence: 99%

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Antitumour and Anti-Inflammatory Effects of Palladium(II) Complexes on Ehrlich Tumour

Quilles

Carli

Ananias

et al. 2013

Zeitschrift Für Naturforschung C

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Palladium(II) complexes are an important class of cyclopalladated compounds that play a pivotal role in various pharmaceutical applications. Here, we investigated the antitumour, anti-infl ammatory, and mutagenic effects of two complexes: [Pd(dmba)(Cl)tu] (1) and [Pd(dmba)(N 3 )tu] (2) (dmba = N,N-dimethylbenzylamine and tu = thiourea), on Ehrlich ascites tumour (EAT) cells and peritoneal exudate cells (PECs) from mice bearing solid Ehrlich tumour. The cytotoxic effects of the complexes on EAT cells and PECs were assessed using the 3-(4,5-dimethylthiazol-3-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. The effects of the complexes on the immune system were assessed based on the production of nitric oxide (NO) (Griess assay) and tumour necrosis factor-α (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10) (ELISA). Finally the mutagenic activity was assessed by the Ames test using the Salmonella typhimurium strain TA 98. Cisplatin was used as a standard. The IC 50 ranges for the growth inhibition of EAT cells and PECs were found to be (72.8 ± 3.23) μM and (137.65 ± 0.22) μM for 1 and (39.7 ± 0.30) μM and (146.51 ± 2.67) μM for 2, respectively. The production of NO, IL-12, and TNF-α, but not IL-10, was induced by both complexes and cisplatin. The complexes showed no mutagenicity in vitro, unlike cisplatin, which was mutagenic in the strain. These results indicate that the complexes are not mutagenic and have potential immunological and antitumour activities. These properties make them promising alternatives to cisplatin.

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Section: Resultsmentioning

confidence: 99%

Section: Organometallic Complexesmentioning

confidence: 99%

Antitumour and Anti-Inflammatory Effects of Palladium(II) Complexes on Ehrlich Tumour

Quilles

Carli

Ananias

et al. 2013

Zeitschrift Für Naturforschung C

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“…Our research group has demonstrated that organopalladated compounds containing diphenylphosphine ligands have activated macrophages in vitro 32 by the immunological activation, in vitro, determining the H 2 O 2 released in cultures of peritoneal macrophage cells from Swiss mice in the presence of organopalladated compounds of the type [Pd(dmba)(X)(dppp)], dmba = N,Ndimethylbenzylamine, dppp = 1,3-bis(diphenylphosphine)propane, X = Cl, N 3 , NCO, NCS. An excellent activation of macrophages by the [Pd(dmba)(X)(dppp)] compounds was observed and the influence of the X ligand on the immune response could be verified.…”

Section: Biological Activitiesmentioning

confidence: 99%

Citotoxicity and immune response induced by organopalladium(II) compounds in mice bearing Ehrlich ascites tumour

Rocha¹,

Santana²,

Ananias³

et al. 2007

J. Braz. Chem. Soc.

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Os ciclometalados de paládio(II) são compostos inorgânicos reativos empregados em vários estudos biológicos devido a seu potencial antitumoral e interação com o sistema imune. Neste estudo, a resposta imune e citotóxica induzida por dois complexos organopaladados: [{Pd(N,C-dmba)} 2 (μ-NCS) 2 ] (1), [Pd(C-dmba)(NCS)(dppp)] (2) [dmba = N,N'-dimetilbenzilamina, dppp = 1,3-bis(difenilfosfino)propano] e cisplatina (cis-DDP), como padrão, foram investigados em camundongos portadores do tumor ascítico de Ehrlich. Os camundongos foram divididos em cinco grupos e inoculados com (1) ou (2) ou cis-DDP ou apenas veículo ou solução salina tamponada de fosfatos (PBS). Diversos parâmetros foram avaliados, tais como a porcentagem de células tumorais presentes no exsudato peritoneal, os níveis de óxido nítrico (NO) e fator de necrose tumoral (TNF-α) séricos e o aumento na expectativa de vida. Os dados obtidos demonstram que o composto (2) apresentou atividade similar à da cis-DDP, como, por exemplo, aumento na expectativa de vida, diminuição dos níveis séricos de TNF-α e aumento da produção de NO.Cyclometallated palladium(II) complexes are reactive inorganic compounds employed in several biological studies because of their antitumour potential and interaction with immune system. In the present study, the immune and citotoxic response induced by two organopalladated complexes: [{Pd(N,C-dmba)} 2 (μ-NCS) 2 ] (1), [Pd(C-dmba)(NCS)(dppp)] (2) [dmba = N,N'-dimethylbenzylamine, dppp = 1,3-bis(diphenylphosphino)propane] and cisplatin (cis-DDP), as standard, were investigated in mice bearing Ehrlich ascites tumour. The mice were divided into five groups and inoculated with the compounds (1) or (2) or cisplatin, or only vehicle or phosphatebuffered saline (PBS). Many parameters were evaluated, such as tumour cell percentage in the peritoneal exsudate, levels of seric nitric oxide (NO) and tumour necrosis factor-alpha (TNF-α) and increase in life span. Analysis of all data revealed, for compound (2), an activity similar to that presented by cisplatin, resulting in increased life span, lower levels of seric TNF-α and increase in NO production.Keywords: palladium(II) complexes, Ehrlich ascites tumour, macrophages, nitric oxide, tumour necrosis factor-alpha. IntroductionCancer is a disease in which unremitting clonal expansion of somatic cells kills by invading, subverting, and eroding normal tissues. 1 Millions of people die every year from the metastatic spread of cancer that occurs through blood and lymphatic vessels or directly into tissues and body cavities. 2 Between the several types of cancer, breast cancer is the most common malignancy in women worldwide. 3 In some cases, immune cells constitute a prominent component of the host response to cancer, but their participation in tumour pathogenesis remains not completely understood. Dense intratumoral lymphocyte cis-Diamminedichloroplatinum(II), 9 a clinically important antitumour drug, acts like a classical alkylating agent in chemotherapy against some types of cancers. It is a potent ...

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“…Our previous results have indicated that the release of H 2 O 2 levels in cultures of activated mice peritoneal macrophages was increased in the presence of compounds of the type [PdX(dmba)(dppp)] (X = Cl, N 3 , NCO, NCS; dmba = N,N-dimethylbenzylamine; dppp = 1,3-bis(diphenylphosphine)propane). 14 Metal-based compounds of 1,2,4-triazole have received attention due to their potential uses as antimicrobial and antitumoral drugs.…”

Section: Introductionmentioning

confidence: 99%

“…As ligands, 1,2,4-triazoles are extremely versatile and display the ability to act as monodentate, exobidentate N(1),N (2) and exobidentate N (2),N(4) when neutral or monodentate, exobidentate N (1),N (2), exobidentate N (1), N(4) and N (1),N (2),N(4)-tridentate when deprotonated. 17 In pursuing our interest on chemical and biological aspects of palladium(II) complexes, 14,18,19 we describe in this work the synthesis, spectroscopic characterization and thermal investigation of the two new 1,2,4-triazolylbridged polynuclear complexes [{PdCl 2 (µ-Htrz)} n ] (1) and [{PdBr 2 (µ-Htrz)} n ] (2). We also investigated the immunomodulatory effects of the compounds 1,2,4-triazole (Htrz), 1, 2, and cisplatin by the release of NO and H 2 O 2 in the culture supernatants of treated murine peritoneal cells.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of palladium(II) complexes of 1,2,4-triazole on murine peritoneal macrophages

Bego¹,

Frem²,

Netto³

et al. 2009

J. Braz. Chem. Soc.

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Os complexos polinucleares [{PdCl 2 (µ-Htrz)} n ] (1) e [{PdBr 2 (µ-Htrz)} n ] (2) (Htrz = 1,2,4-triazol) foram sintetizados neste trabalho. O composto 1 foi preparado a partir da substituição da acetonitrila do precursor [PdCl 2 (MeCN) 2 ] pelo 1,2,4-triazol. A adição posterior de brometo de potássio ao meio reacional resultou na formação do complexo 2. Os complexos inéditos foram isolados, purificados e caracterizados por análise elementar, espectroscopias vibracional no infravermelho e eletrônica no UV-visível e curvas de análise termogravimétrica (TG). Os resultados experimentais sugerem que, em ambos os casos, a coordenação do 1,2,4-Htrz ocorra via átomos N(2) e N(4), atuando como pontes entre centros de paládio. O poliedro de coordenação quadrado-planar ao redor do paládio(II) é determinado pelos dois átomos de N do heterociclo e por dois ligantes cloro (1) ou bromo (2), em uma provável configuração trans. As curvas TG indicaram que a natureza do ligante aniônico não afeta significativamente a estabilidade térmica de 1 e 2. Os produtos finais de decomposição térmica foram identificados como paládio metálico pela técnica de difratometria de raios X de pó. Testes preliminares envolvendo a avaliação dos efeitos dos compostos 1, 2 e Htrz na produção de H 2 O 2 e NO em culturas de macrófagos peritoneais de camundongos BALB/c foram realizados in vitro.The 1,2,4-triazolyl-bridged polynuclear complexes [{PdCl 2 (µ-Htrz)} n ] (1) and [{PdBr 2 (µ-Htrz)} n ] (2) have been obtained in this work. Compound 1 is prepared by the displacement of acetonitrile from [PdCl 2 (MeCN) 2 ] by 1,2,4-triazole (Htrz). Further addition of potassium bromide to the reaction medium afforded complex 2. The new complexes have been isolated, purified and characterized by means of elemental analysis, IR and UV-visible electronic spectroscopies and thermogravimetric (TG) curves. The experimental data suggested that, in both cases, the coordination of 1,2,4-Htrz takes place through the N(2) and N(4) atoms, bridging the palladium centers. The square-planar coordination polyhedron of palladium(II) is determined by two nitrogen atoms from the triazole ligands, while the other two coordination positions are occupied by the chloro (1) or bromo (2) ligands. TG curves indicated that the nature of the anionic ligand does not affect significantly the thermal stability of 1 and 2. The final products of the thermal decompositions were identified as metallic palladium by X-ray powder diffractometry. Preliminary tests involving the evaluation of the effects of compounds 1, 2 and Htrz on H 2 O 2 and NO production in cultures of peritoneal macrophages from BALB/c mice were carried out in vitro.

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Emprego de compostos organometálicos mononucleares de paládio(II) na ativação de macrófagos peritoneais de camundongos

Cited by 17 publications

References 11 publications

Antitumour and Anti-Inflammatory Effects of Palladium(II) Complexes on Ehrlich Tumour

Antitumour and Anti-Inflammatory Effects of Palladium(II) Complexes on Ehrlich Tumour

Citotoxicity and immune response induced by organopalladium(II) compounds in mice bearing Ehrlich ascites tumour

Immunomodulatory effects of palladium(II) complexes of 1,2,4-triazole on murine peritoneal macrophages

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