A microsatellite library for Carica papaya L. cv. Sunrise solo

Abstract

-In experimental areas of the Education and Researches Ilha Solteira and Jaboticabal UNESP/Campus farms were selected and tagged 20 hermaphrodite plants and 20 feminine of cultivar Sunrise Solo, Improved Sunrise Solo cv.72/12 and Baixinho of Santa Amália.The seeds origined of the selected fruits were cropped to be analysed the self-pollination efficiency and frequency of the sex in the progenies. After that, samples of the young leaf of the matrix plants were colected for the extration of the DNA. It was built five library enriched of microsatellite sequencies, using probes (TCA) 10 , (TC) 13 , (GATA) 4 , (CAC) 10 e (TGAG) 8 .It was possible the development of the primers only in the library that has utilized the probe (TCA) 10. This probe allowed the design of 32 primer pairs. From these, 31 presented pattern of unique band in agarose Metaphor and in acrilamide. For primer S36 were observed 2 bands, but with no polymorphism to differentiation in the sexual form at papaya tree culture. However, these primers can be tested, in the futures, in the investigation of the others features in segregated populations of this specie and the related species, germoplasm analysis, cultivars identification, parent evolution and molecular markers for the assisted plant breeding programs.

Genetic characterization of papaya plants (Carica papaya L.) derived from the first backcross generation

“…For the amplification reactions with the SSR marker, we selected 32 primers used by Santos et al (2003), and 45 clones containing microsatellite sequences developed by Pérez et al (2006), available at GenBank (http://www.ncbi.nlm.nih.gov). The clones containing the DNA sequence that flanks the microsatellite region were used to design the primer pairs (forward and reverse), using the programs Expression version 1.0.0.0 and Oligo version 6.68 (Genamics).…”

Polymorphic microsatellite marker set for Carica papaya L. and its use in molecular-assisted selection

“…SSRs have other advantages as well, such as high reproducibility, technical simplicity, low cost, high resolution power and, most important of all, codominance (Brondani et al 1998;Rallo et al 2000;Oliveira et al 2006). Microsatellites were first reported in C. papaya L. by Sharon et al (1992), Parasnis et al (1999), Santos et al (2003), Oliveira et al (2008), and Eustice et al (2008). Reliable and highly polymorphic SSR markers have been used mainly for genetic map construction (Chen et al 2007) and sexual differentiation (Parasnis et al 1999;Santos et al 2003) and to access genetic diversity (Ocampo Pérez et al 2007).…”

Early sex discrimination in Carica papaya by nuclei FISH

“…Therefore, the sex type identification in this species is only possible after flowering (Deputy et al 2002), which may take 5-8 months (Parasnis et al 1999(Parasnis et al , 2000Ma et al 2004). The selection of the appropriate papaya sex type prior to planting in the field is of great interest by growers, for reducing the production costs (cultivation area, labor, time, financial resources and others) (Parasnis et al 1999;Santos et al 2003;Costa et al 2011;Reddy et al 2012).…”

“…The advantages of DNA markers include their abundance and stability, a relatively high rate of polymorphism in many populations, usually routine technologies for scoring, frequent comparability across species, clear dominance or codominance in most situations, presence at all stages and tissue types of plant growth, lack of epistasis and no detectable phenotypic effect (Kanchana-Udomkan et al 2014). Although molecular markers could be substantial tool for researchers to employ in sex determining of experimental material, some authors contest its practicability in routine sex testing by farmers, since that is an expensive and intensive technology (Deputy et al 2002;Santos et al 2003). …”