Freqüência de híbridos em cruzamento entre tangerina 'cravo' e laranja 'pêra': análise de marcadores morfológicos e RAPD

Oliveira, R. P. de; Novelli, Valdenice M.; Machado, Marcos Antônio

doi:10.1590/s0100-204x2000000900024

Cited by 17 publications

(19 citation statements)

References 15 publications

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“…In Brazil, the annual losses in citrus production due to CVC are estimated to be 235 millions dollars (Fundecitrus, 2000). In Centro Apta Citros 'Sylvio Moreira', São Paulo State, a hybrid progeny between 'Cravo' mandarin and 'Pêra' sweet orange was obtained by controlled crossings (Oliveira et al, 2000). Therefore, that progeny and the genetic maps of 'Pêra' sweet orange and 'Cravo' mandarin may help to understand the inheritance of CVC and other important traits.…”

Section: Introductionmentioning

confidence: 99%

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers

Oliveira

Cristofani²,

Machado³

2004

Pesq. agropec. bras.

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-The objective of this work was to construct linkage maps of 'Pêra' sweet orange [Citrus sinensis (L.) Osbeck] and 'Cravo' mandarin (Citrus reticulata Blanco) using RAPD markers and the pseudo-testcross strategy. The parents were chosen according to the resistance/susceptibility to citrus variegate chlorosis (CVC). The segregation of 176 markers was analyzed in 94 progeny of F 1 hybrids, which were obtained from controlled crossings. The linkage map of 'Pêra' sweet orange had 117 markers defined by 12 linkage groups, which spanned 612.1 cM. Only six markers could not be linked to the linkage group and 48.7% of the markers showed segregation distortion. The linkage map of 'Cravo' mandarin had 51 markers defined by 12 linkage groups, which spanned 353.3 cM. Only two markers did not link to the groups and 15.7% showed segregation distortion. The construction of linkage maps is relevant to future mapping studies of the inheritance of CVC, citrus canker and leprosis.

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Section: Introductionmentioning

confidence: 99%

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers

Oliveira

Cristofani²,

Machado³

2004

Pesq. agropec. bras.

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“…significativamente maiores, quando comparado com outros marcadores moleculares a exemplo do RAPD (Cristofani & Machado, 1998;Oliveira et al, 2000;Cristofani et al, 2001).…”

Section: Resultsunclassified

Identificação de híbridos de citros resistentes à mancha-marrom-de-alternária por meio de fAFLP e testes de patogenicidade

Chagas

Cazetta

Lemos

et al. 2007

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi identificar híbridos, oriundos de hibridações controladas entre 'Folha Murcha' x 'Ponkan' e testá-los quanto à resistência a Alternaria alternata f. sp. citri. As plântulas foram obtidas via cultura in vitro de embriões. Utilizou-se o marcador molecular fAFLP para identificação dos híbridos e, em seguida, realizou-se o teste de patogenicidade nos híbridos com isolados de Alternaria alternata f. sp. citri, em condições de laboratório. Os pares de primers EcoRI AAG -MseI CAG e EcoRI ACC -MseI CAA foram os mais eficientes na identificação dos híbridos, os quais identificaram 48,5% de híbridos. Os híbridos F64, F108, F111, F113, F131 e F139 são potencialmente resistentes a Alternaria alternata f. sp. citri.Termos para indexação: Citrus sinensis, Citrus reticulata, melhoramento genético, biotecnologia vegetal, marcador molecular. Alternaria brown spot resistant citrus hybrid identification by means of fAFLP and pathogenicity testsAbstract -The objective of this work was to identify hybrids obtained from controlled crossings between 'Folha Murcha' x 'Ponkan', and to check their resistance to Alternaria alternata f. sp. citri. The seedlings were obtained by in vitro embryo culture. The fAFLP marker technique was used to identify the hybrids, then laboratory pathogenicity test of the hybrids was accomplished with Alternaria alternata f. sp. citri isolates. The pairs of primers EcoRI AAG -Msel CAG and EcoRI ACC -Msel CAA were the most efficient for hybrids identification, and distinguished 48.5% of hybrids. Hybrids F64, F108, F111, F113, F131 and F139 showed potential resistance to the Alternaria alternata f. sp. citri.

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“…The hybrid population was differentiated from the nucellar plants with morphologic and RAPD markers according to Oliveira et al [15].…”

Section: Plant Materialsmentioning

confidence: 99%

Integrated genetic map of citrus based on RAPD markers

Cristofani

Machado²

2005

Fruits

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Integrated genetic map of citrus based on RAPD markers.Abstract --Introduction. The citrus industry is one of the main activities of Brazilian agriculture. Several pests and diseases have threatened citrus culture in recent years. Genetic mapping is one of the most efficient strategies for conducting advanced genetic studies by facilitating plant selection guided by markers. The objective of this study was to construct an integrated genetic map between Citrus sinensis (L.) Osbeck cv. 'Pêra' and C. reticulata Blanco cv. 'Cravo' using two different types of segregation of markers. Materials and methods. The linkage analyses were conducted with segregation data obtained from 256 RAPD markers in a population of 94 hybrids. Analyses were performed through the software JoinMap, LOD ≥ 6.0, θ ≤ 0.25, and the Kosambi function. Results and discussion. The linked map between the two cultivars had 217 markers defined by 15 linkage groups, covering 527 cM. This map had five linkage groups with common markers of 'Pêra' and 'Cravo'. The inclusion of new markers would increase the number of linkage groups with markers from both parents, thus making their number equal to the haploid chromosome number. The alterations in the order and in the distances among the markers, due to the presence or absence of bridging markers in the linkage map, are discussed. Citrus reticulata / Citrus sinensis / genetic maps / genetic markers / RAPDCarte génétique intégrée des agrumes, basée sur l'utilisation de marqueurs RAPD.Résumé --Introduction. L'industrie des agrumes est l'une des activités principales de l'agriculture brésilienne. Plusieurs ravageurs et maladies ont menacé cette culture ces dernières années. La cartographie moléculaire est l'une des stratégies les plus efficaces pour entreprendre des études génétiques avancées afin de faciliter une sélection des plants assistée par marqueurs. L'objectif de cette étude a été de construire une carte génétique intégrée entre Citrus sinensis (L.) Osbeck cv. 'Pêra' et C. reticulata Blanco cv. 'Cravo' en utilisant deux types différents de ségrégation des marqueurs. Matériel et méthodes. Les analyses de liaisons ont été conduites à partir de données de ségrégation obtenues de 256 marqueurs RAPD dans une population de 94 hybrides. Les analyses ont été réalisées avec le logiciel JoinMap. Résultats et discussion. La carte des liaisons entre l'un et l'autre des cultivars a comporté 217 marqueurs définis par 15 groupes de liaisons, couvrant 527 cM. Cette carte a présenté cinq groupes de liaisons contenant les marqueurs communs de Pêra et Cravo. L'inclusion de nouveaux marqueurs augmenterait le nombre de groupes de liaisons portant des marqueurs des deux parents, en rendant leur nombre égal au nombre de chromosomes haploïde. Les différences d'ordre et de distances au sein des marqueurs, dues à la présence ou à l'absence de marqueurs dans la carte de liaisons, sont discutées. Citrus reticulata

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Freqüência de híbridos em cruzamento entre tangerina 'cravo' e laranja 'pêra': análise de marcadores morfológicos e RAPD

Cited by 17 publications

References 15 publications

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers

Genetic linkage maps of 'Pêra' sweet orange and 'Cravo' mandarin with RAPD markers

Identificação de híbridos de citros resistentes à mancha-marrom-de-alternária por meio de fAFLP e testes de patogenicidade

Integrated genetic map of citrus based on RAPD markers

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