Metallo-β-lactamase-production in meropenem-susceptible Pseudomonas aeruginosa isolates: risk for silent spread

Picão, Renata C.; Carrara-Marroni, Floristher Elaine; Gales, Ana Cristina; Venâncio, Emerson José; Xavier, Danilo Elias; Tognim, Maria Cristina Bronharo; Pelayo, Jacinta Sánchez

doi:10.1590/s0074-02762012000600007

Cited by 15 publications

(12 citation statements)

References 20 publications

(29 reference statements)

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“…The differences observed here between the phenotypic and genotypic methods of detection of MBLs may be because MBL producers might act as silent reservoirs of such resistance determinants [26]. …”

Section: Discussionmentioning

confidence: 99%

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Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

et al. 2014

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Background: Pseudomonas aeruginosa commonly causes nosocomial bloodstream infections and the emergence of a variety of β-lactamases (BLs) is worrying. In 5 hospitals in Belo Horizonte, Brazil, the presence of phenotypes encoding BL genes was established and the genetic diversity of the P. aeruginosa strains recovered from bloodstream infections was analyzed. Materials and Methods: The isolates were investigated using a disk diffusion (DD) method and the Etest®, for encoding metallo-β-lactamases (MBLs), oxacillinases and cephalosporinases. Genes and genetic diversity were evaluated by random amplified polymorphic DNA (RAPD) genotyping and enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results: Twelve strains (30%) were positive for MBLs by Etest and DD, 15 were cephalosporinase-positive and 87.5% were positive for bla_SPM-1 and bla_VIM-1. Twenty-three strains (57.5%) were grouped into profile A, 32.5% into profile B and 10% into profile C by RAPD genotyping. ERIC-PCR revealed a varying degree of similarity between strains, ranging from 45 to 100%. Conclusions: The results suggest distinct clonal populations in the 5 hospitals studied, indicating a potentially problematic epidemiological situation in Belo Horizonte, Brazil.

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Section: Discussionmentioning

confidence: 99%

“…aeruginosa [8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27]. In this study, PCR assays were used to evaluate the presence of carbapenem-hydrolyzing class D BL and MBL genes, while ERIC-PCR and RAPD genotyping were performed to identify and establish the relatedness between the isolates.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

et al. 2014

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“…() and Picão et al . (), the combination of many mechanisms may be necessary to achieve antimicrobial resistance, or these samples may even exhibit a higher permeable membrane, allowing drug action.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, K. pneumoniae had positive results for the bla TEM and bla SHV genes, but revealed a phenotype of resistance only to penicillin. According to Pellegrino et al (2008) and Picão et al (2012), the combination of many mechanisms may be necessary to achieve antimicrobial resistance, or these samples may even exhibit a higher permeable membrane, allowing drug action.…”

Section: Antimicrobial Resistancementioning

confidence: 99%

Phenotypic and genotypic characterization of clinically relevant bacteria isolated from dental waste and waste workers’ hands, mucosas and coats

Tagliaferri

Vieira

Carvalho

et al. 2017

Lett Appl Microbiol

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Infectious dental waste can contain clinically relevant bacteria with important resistance and biofilm profiles. These micro-organisms could be transmitted to waste workers, other professionals and patients if the principles of biosafety measures are neglected. To our knowledge, no study has ever evaluated the microbial characterization and the potential contamination risk of dental infectious waste and waste handlers. The presence of clinically relevant bacteria in the hands and nasal mucosa of waste workers highlights the need for studies in this field to clarify the risk of these pathogens in dental healthcare services, and to stress the need for an efficient waste management.

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“…Detection of MβL-producing P. aeruginosa is important for controlling the dissemination of MβL isolates and for the correct choice of antimicrobial regimens; these enzymes are able to hydrolyze most β-lactams but do not always exhibit carbapenem resistance (12) , as observed in one of the two SPM-1-positive isolates. This result emphasizes the utilization of ceftazidime resistance as a criterion for selecting isolates for phenotypic MβL production tests.…”

mentioning

confidence: 99%

Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

Costa

Fleming

Paula

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction: Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidimeresistant P. aeruginosa isolates from State of Sergipe, Brazil. Methods: Metallo-β-lactamase was investigated using the disk approximation test and polymerase chain reaction (PCR). Genetic diversity was evaluated by pulsed-fi eld gel electrophoresis (PFGE). Results: A total of 48 (51.6%) isolates were resistant to ceftazidime. Six (12.2%) of these were positive for metallo-β-lactamase production. Only two (4.1%) of the ceftazidime-resistant isolates carried the bla SPM-1 gene. Conclusions: Production of metallo-β-lactamases was not the main mechanism of resistance to ceftazidime and carbapenems among P. aeruginosa strains in Sergipe, Brazil.

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Metallo-β-lactamase-production in meropenem-susceptible Pseudomonas aeruginosa isolates: risk for silent spread

Abstract: Relative expression of efflux pumps genes, oprD, ampC and bla . Data is represented as mean (n-fold) and 95% confidence interval.

Cited by 15 publications

References 20 publications

Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

Phenotypic and genotypic characterization of clinically relevant bacteria isolated from dental waste and waste workers’ hands, mucosas and coats

Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

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