Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

Abstract

Introduction: Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidimeresistant P. aeruginosa isolates from State of Sergipe, Brazil. Methods: Metallo-β-lactamase was investigated using the disk approximation test and polymerase chain reaction (PCR). Genetic diversity was evaluated by pulsed-fi eld gel electrophoresis (PFGE). Results: A total of 48 (51.6%) isolates were resistant to ceftazidime. Six (12.2%) of these were positive for metallo-β-lactamase production. Only two (4.1%) of the ceftazidime-resistant isolates carried the bla SPM-1 gene. Conclusions: Production of metallo-β-lactamases was not the main mechanism of resistance to ceftazidime and carbapenems among P. aeruginosa strains in Sergipe, Brazil.

Clinical and Molecular Epidemiology of Multidrug-Resistant P. aeruginosa Carrying aac(6')-Ib-cr, qnrS1 and blaSPM Genes in Brazil

“…aeruginosa clone SP/ST277, considered a high-risk clone [ 7 , 18 , 59 , 60 ]. The bla SPM-1 gene was first detected in São Paulo, and later in others cities in Brazil, moreover several studies have shown its global spread and pandemic potential, causing important morbidity and mortality in hospital infections [ 18 , 43 , 60 , 61 ]. In our study, we observed a high frequency of SPM among the isolates, especially those belonging to clone A.…”

Identification of São Paulo metallo-beta-lactamase-1-producing Pseudomonas aeruginosa in the Central-West region of Brazil: a case study

“…SPM-1 was first detected and reported in 2001 in São Paulo, Brazil 1 . Since then, SPM-1-producing P. aeruginosa strains have been reported in different regions of Brazil 3,4 . However, data are lacking regarding the clinical features of SPM-1-related drug resistance in the Central Western Brazilian State of Mato Grosso do Sul.…”

Epidemiologia e caracterização molecular de Pseudomonas aeruginosa, Klebsiella pneumoniae e Escherichia coli multirresistentes carreando determinantes de resistência às quinolonas mediada por plasmídeos

“…Além da multirresistência, os dados observados mostraram que a resistência aos carbapenêmicos é muito alta, ratificando relatos do aumento significativo das frequências de resistência na América Latina, onde sua disseminação está relacionada a clones dominantes e a produção de enzimas do tipo MBL (ANDRADE et al, 2003;BAUMGART;MOLINARI;SILVEIRA, 2010;SCHEFFER et al, 2010b;AKYA et al, 2015). No Brasil, o gene predominante que codifica MBL é bla SPM-1 , disseminado pelo clone SP/ST277 (GALES et al, 2003;WRIGHT et al, 2014;COSTA et al, 2015;FONSECA et al, 2015), detectado pela primeira vez em São Paulo, e que atualmente se espalhou por diferentes regiões geográficas do paísdevido ao seu potencial pandêmico, e está associado a frequências elevadas de morbidade e mortalidade (SILVA et al, 2014;COSTA et al, 2015;GALETTI et al, 2015;FONSECA et al, 2015). Em nosso estudo, foi observada frequência da enzima do tipo SPM, principalmente nas amostras do clone A, além de duas amostras com o gene bla VIM .…”