Evaluation of four methods for detecting the beta-lactamase activity in Neisseria gonorrhoeae isolated in Cuba

Llanes, Rafael; González, Miriam; Martínez, Isabel de Miguel; Sosa, Jorge; Guzmán, Daymi; Gutiérrez, Óscar; Llop, Alina; Sánchez, Lizet

doi:10.1590/s0074-02762003000800020

Cited by 9 publications

(5 citation statements)

References 13 publications

(8 reference statements)

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“…The isolates showed differences in the decolorization time from those with less inhibition diameters in penicillin sensitivity test than the other, were the faster in the reduction of iodine. These results agreed with Lianes et al [28].…”

Section: Concerningsupporting

confidence: 93%

Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Attia

Hassan

Elwan

2017

Zagazig Veterinary Journal

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Beta-lactamases (bls) are implicated seriously in resistance to β -lactam antibiotics. β-lactam resistance related to Sulfa hydral variabl-beta lactamases (SHV-bls) were detected in E. coli and Klebsiellae isolated from human and chickens. In this work, resistance profile of the isolates, extended spectrum β-lactamases (ESBLs) activity and SHV genes sequencing were examined. E. coli was isolated from humans, broiler and day-old chicks samples, meanwhile, K. pneumonia was isolated only from human samples (88.8%). Sixty-four (88.8 %) of human isolates contained betalactamases (iodometric positive), 28% were ESBLs and 35% contained SHV. In case of day-old chick isolates, 50% contained beta-lactamases (iodometric positive), 5.5% were ESBLs. Out of 45 broiler isolates, 37.7% contained beta-lactmases (iodometric positive), 8.8% were ESBLs and 80% contained SHV. It was found that SHV were of SHV-5 and SHV-12. It could be concluded that, wide spread of ESBLs SHV-5 and SHV-12 in humans and chicken is a major concern in resistance to β -lactam drugs.

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Section: Concerningsupporting

confidence: 93%

Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Attia

Hassan

Elwan

2017

Zagazig Veterinary Journal

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“…In other countries, higher levels of b-lactamase production are usually identified. [12][13][14][15] However, in a previous Russian study, a low level of b-lactamase positivity (2%) was also identified. 16 This may indicate that treatment with b-lactams is no longer widespread in Russia or even that endemic spread of the b-lactamase-producing strains has not been established in Russia during the past few years.…”

Section: Discussionmentioning

confidence: 81%

Antibiotic susceptibility of Neisseriagonorrhoeae in Arkhangelsk, Russia

Vorobieva

Firsova²,

Абабкова³

et al. 2006

Sexually Transmitted Infections

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Objectives: To characterise comprehensively the antibiotic susceptibility of Neisseria gonorrhoeae in Arkhangelsk, Russia, and to investigate whether the recommended treatment guidelines are updated and effective. Methods: The susceptibility of N gonorrhoeae isolates, cultured during June-November 2004 mainly from consecutive patients with gonorrhoea (n = 76) in Arkhangelsk, to penicillin G, ampicillin, cefixime, ceftriaxone, ciprofloxacin, erythromycin, azithromycin, kanamycin, spectinomycin and tetracycline was analysed using Etest. Nitrocefin discs were used for b-lactamase detection. Results: The levels of intermediate susceptibility and resistance to the different antibiotics were as follows: penicillin G 76%, ampicillin 71%, cefixime 0%, ceftriaxone 3%, ciprofloxacin 17%, erythromycin 54%, azithromycin 14%, kanamycin 49%, spectinomycin 0% and tetracycline 92%. Of the isolates 55 (72%) were determined as multiresistant-that is, they showed intermediate susceptibility or resistance to three or more classes of antibiotics. However, none of the isolates were b-lactamase producing. Conclusions: In Arkhangelsk, and presumably in many other areas of Russia, penicillins, ciprofloxacin, erythromycin, azithromycin, kanamycin and tetracycline should not be used in the treatment of gonorrhoea if the results of antibiotic susceptibility testing are not available. In Russia, optimised, standardised and qualityassured antibiotic susceptibility testing needs to be established in many laboratories. Subsequently, continuous local, regional and national surveillance of antibiotic susceptibility is crucial to detect the emergence of new resistance, monitor changing patterns of susceptibility and be able to update treatment recommendations on a regular basis.A s a result of the break-up of the Soviet Union, during the past decade, Russia and the other newly independent states have gone through major changes, which have considerably influenced the healthcare system too. Regarding the incidence of gonorrhoea, reliable figures are still lacking mainly because of suboptimal diagnostics, incomplete epidemiological surveillance and reporting of new cases, as well as self-medication.1 Furthermore, the prevalence of antibiotic resistance of Neisseria gonorrhoeae is unknown in most Russian areas, and this knowledge is crucial for empirical treatment. Owing to the worldwide rapid increase of antibiotic resistance, empirical treatment strategies need constant re-evaluation, 2 and antibiotic susceptibility testing is the main tool for updating them.3 Consequently, it is also essential to monitor the level of antibiotic resistance of N gonorrhoeae in Russia.1 4 As in most of the east European countries, it is crucial to establish local, regional and national antibiotic resistance networks and, in addition, monitor antibiotic consumption in Russia.5 During the 1990s, controlled, centralised provision of antibiotics and/or antibiotic resistant surveillance ended and the consumption of antibiotics increased markedly in Russia. 6The Arkhangels...

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“…In this study determination of βlactamase production achived by microiodometric assay. This method is sensitive for measuring the rate of hydrolysis of penicillin to penicilloic acid by β-lactamase and depended upon the reduction of iodine by penicilloic acid but not by penicillin and it was carried out by measuring the rate of decolourization of the blue starch iodine complex when the enzyme and substrate react in the presence of the starchiodine (8) . The results of β-lactamase detection reveald that 86.7% liver isolates and 78.6% of lung isolates produce this enzyme but there was variation in their decolourization times.…”

Section: Discussionmentioning

confidence: 99%

“…Rapid iodometric method was used for detection of β-lactmase as described by (Lian et al,) (8) . As a follow: 2) A 0.1ml of pencilline solution was added to the wells of microtiter plates.…”

Section: -Detection Of β-Lactamase Productionmentioning

confidence: 99%

Prevalence of Β- Lactamase Producing Staphylococcus Aureus in Slaughtered

Rodhan¹

2008

Bas.J.Vet.Res.

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Fifty liver and lung samples (25 for each) were examend for the presence of Staphylococcus aureus. The rate of S. aureus isolates were 60 and 56% in livers and lungs respectively. Identification of S. aureus isolates were performed by studying, it's cultural characteristics on mannitol salt agar and testing their pathogenicity factors (Coagulase, haemolysis and B-lactmase production), 72.4% of isolates were coagulase positive, 58.6% were βlactamase producer, 62.1% were β-haemolytic and 37.9% were α-haemolytic. There was a difference in the ablity of S. aureus isolates to produce B-lactamase concerning the time of decolourization when iodometric assay was used.

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Evaluation of four methods for detecting the beta-lactamase activity in Neisseria gonorrhoeae isolated in Cuba

Abstract: Four methods (chromogenic, acidimetric, inhibition, and iodometric)

Cited by 9 publications

References 13 publications

Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Antibiotic susceptibility of Neisseriagonorrhoeae in Arkhangelsk, Russia

Prevalence of Β- Lactamase Producing Staphylococcus Aureus in Slaughtered

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