Fifty liver and lung samples (25 for each) were examend for the presence of Staphylococcus aureus. The rate of S. aureus isolates were 60 and 56% in livers and lungs respectively. Identification of S. aureus isolates were performed by studying, it's cultural characteristics on mannitol salt agar and testing their pathogenicity factors (Coagulase, haemolysis and B-lactmase production), 72.4% of isolates were coagulase positive, 58.6% were βlactamase producer, 62.1% were β-haemolytic and 37.9% were α-haemolytic. There was a difference in the ablity of S. aureus isolates to produce B-lactamase concerning the time of decolourization when iodometric assay was used.
One Hundred eighty fecal samples and (50) bile samples were collected from cattle of different ages and both sexes present in Basrah farms and Slaughterhouse. The results of the bacteriological and serological methods carried out on fecal and bile samples of cows detect Salmonella spp in the fecal samples of 3 cows ( 6611 ) % and these bacteria were not detected (0%) in bile samples. Concerning the effect of months of study on the rate of Salmonella spp. isolation . The higher rate of isolation was encountered in march (6.66%) followed by February (2.38%), while in other months no Salmonella isolates were observed. Depending on the sex of animals the higher rate of Salmonella isolation was observed in males )%2.06) and it was in females (1.204%) . According to age group the higher rate of Salmonella isolation (%965)was observed in the third age group ) followed by the second age group (1<-3) in which the rate was ( %2.09) . There was statistical significance difference (p< 0.05) among age groups concerning the Salmonella isolation rate. Suckling mice and permeability of rabbit skin were show good result for detection of enterotoxin which were extracted from the more virulent isolate No. (161). The enterotoxin then were purified and fractionated by gel flirtation on sephadex (G-100). Results of gel flirtation showed that the toxin had two peaks , one of them were highly toxic. The chemical studying of enterotoxin characteristics revealed that it contained sugar moiety and it was a glycoprotein.
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