Molecular Characterization of β-lactamase Genes in Antibiotic Resistant Bacteria

Abstract

Beta-lactamases (bls) are implicated seriously in resistance to β -lactam antibiotics. β-lactam resistance related to Sulfa hydral variabl-beta lactamases (SHV-bls) were detected in E. coli and Klebsiellae isolated from human and chickens. In this work, resistance profile of the isolates, extended spectrum β-lactamases (ESBLs) activity and SHV genes sequencing were examined. E. coli was isolated from humans, broiler and day-old chicks samples, meanwhile, K. pneumonia was isolated only from human samples (88.8%). Sixty-four (88.8 %) of human isolates contained betalactamases (iodometric positive), 28% were ESBLs and 35% contained SHV. In case of day-old chick isolates, 50% contained beta-lactamases (iodometric positive), 5.5% were ESBLs. Out of 45 broiler isolates, 37.7% contained beta-lactmases (iodometric positive), 8.8% were ESBLs and 80% contained SHV. It was found that SHV were of SHV-5 and SHV-12. It could be concluded that, wide spread of ESBLs SHV-5 and SHV-12 in humans and chicken is a major concern in resistance to β -lactam drugs.