Use of polymerase chain reaction and enzymatic cleavage in the identification of Helicobacter spp. in gastric mucosa of human beings from North Paraná, Brazil

Camargo, Pedro Luiz Teixeira de; Alfieri, A.A.; Bracarense, Ana Paula Frederico Rodrigues Loureiro; Menoli, R.; Spinosa, S; Hagiwara, Mitika Kuribayashi

doi:10.1590/s0074-02762003000200016

Cited by 7 publications

(7 citation statements)

References 20 publications

(26 reference statements)

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“…This result suggests that coinfection with other Helicobacter species does not significantly influence H. pylori's propensity to colonize the gastric environment. These results show that there is a higher incidence of H. felis in the African population than in Europe (53) or South America (7). Whether this is due to specific host factors or environmental and social factors remains to be elucidated.…”

Section: Discussionmentioning

confidence: 77%

“…H. felis is a gram-negative, spiral bacterium that measures approximately 0.4 by 5 to 10 m (20). Reports of H. felis in humans are, however, rare, and recent studies failed to show the organism in human gastric biopsy specimens (7). The principal Helicobacter infection in humans is H. pylori, with infection rates in developing countries reaching 70 to 90% (2,11); however, prevalence rates are decreasing in the developed world (3,18,40).…”

mentioning

confidence: 99%

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Incidence of Helicobacter felis and the Effect of Coinfection with Helicobacter pylori on the Gastric Mucosa in the African Population

Fritz

Slavík²,

Delport

et al. 2006

J Clin Microbiol

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Helicobacter pylori and Helicobacter felis are two of the Helicobacter spp. that infect humans. H. pylori has been linked to significant gastric pathology. Coinfection with Helicobacter spp. may influence infectious burden, pathogenesis, and antibiotic resistance; however, this has not been studied. The aims of this study were to identify the incidence of H. felis and to analyze the effects of coinfection with both organisms on gastric pathology in a well-characterized South African population. Biopsy samples from the gastric corpora and antra of volunteers (n ‫؍‬ 90) were subjected to histological examination and PCR for the identification of H. pylori and H. felis. We further investigated the effect of global strain type on the occurrence of precursor lesions by assigning nucleotide sequences derived from PCR amplification of three genes to global groupings (ancestral Africa1, ancestral Africa2, ancestral Europe, ancestral Asia, and mixed). H. pylori was detected in 75 (83.3%), H. felis in 23 (25.6%), and coinfection in 21 (23.3%) of the volunteers by PCR. H. felis was randomly distributed among adults and children but clustered within families, suggesting intrafamilial transmission. Analysis of histopathology scores revealed no differences in atrophy, activity, and helicobacter density between H. felispositive and H. felis-negative volunteers. H. pylori substrains common to southern Africa showed no differences in inflammation or atrophy scores. The incidences of H. felis and coinfection with H. pylori in the African population are high. H. felis infection, however, does not influence specific gastric pathology in this population.

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Section: Discussionmentioning

confidence: 77%

mentioning

confidence: 99%

Incidence of Helicobacter felis and the Effect of Coinfection with Helicobacter pylori on the Gastric Mucosa in the African Population

Fritz

Slavík²,

Delport

et al. 2006

J Clin Microbiol

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“…PCR amplifications were performed in a final volume of 20 ml containing 100 ng of extracted DNA, 2 ml of 10Â PCR buffer (Fermentas, Lithuania), 0.2 mM of dNTP, 1.5 mM MgCl 2 , 25 pM/ml of each primer and 0.5 U of Taq DNA polymerase (Fermentas, Lithuania) under conditions previously described. 16 Gastric biopsies were first tested against genus-specific primers (16S rRNA), then assessed for colonisation with H pylori, H felis, or H heilmannii via strain-specific primers (Table 1). 17e19 The resulting PCR products underwent gel electrophoresis (1.5% (w/v) agarose gel with 0.3% ethidium bromide in 10% Triseborate ethylenediaminetetraacetic acid (EDTA) buffer (TBE)) and visualised under an ultraviolet transilluminator.…”

Section: Dna Extraction and Pcr Assaysmentioning

confidence: 99%

The efficacy and safety of long-term Helicobacter species quadruple therapy in asymptomatic cats with naturally acquired infection

Khoshnegah

Jamshidi

Mohammadi

et al. 2011

Journal of Feline Medicine and Surgery

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Owing to rising drug-resistant Helicobacter species infections in people and animals, currently therapies are losing their efficacy; therefore, regimens efficacious in the presence of drug resistance are needed. This study assessed the efficacy and safety of a 14-day quadruple Helicobacter species therapy in cats with naturally acquired infection. Thirteen asymptomatic adult stray cats with Helicobacter species infection (identified by analysis of gastric biopsies using polymerase chain reaction and Helicobacter-specific primers) received omeprazole 0.7mg/kg q 8h plus amoxicillin 20mg/kg q 12h, metronidazole 20mg/kg q 12h and clarithromycin 7.5mg/kg q 12h, for 14 days. Second molecular analysis of gastric biopsies revealed persistence of Helicobacter species DNA in four cats that were negative on quantitative urease testing, cytology and histopathology. Our results suggest that antibiotic regimens that are effective against Helicobacter pylori in people cannot eradicate Helicobacter species in cats with naturally acquired infection, although transient suppression may occur.

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“…Helicobacter species, the primer sequences, the annealing temperatures, and the size (base pairs) of amplified fragments were described in a previous study (2). Spearman test was performed to verify the correlation between colonization density and inflammation (18).…”

Section: The Genes Targeted For Helicobacter Genus Andmentioning

confidence: 99%

Gastric Helicobacter spp. infection in captive neotropical Brazilian feline

Camargo¹,

Uenaka²,

Motta³

et al. 2011

Braz. J. Microbiol.

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Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus, corpus and pyloric antrum were evaluated for the presence of Helicobacter species.Warthin-Starry (WS) staining and PCR assay with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant association between Helicobacter infection and histological findings was verified.These observations suggest that gastric Helicobacter spp. could be a commensal or a eventual pathogen to captive neotropical feline, and that procedures, way life, and stress level on the shelter apparently had no negative repercussion over the integrity of the stomach.

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Use of polymerase chain reaction and enzymatic cleavage in the identification of Helicobacter spp. in gastric mucosa of human beings from North Paraná, Brazil

Abstract: Helicobacter pylori is the most common gastric bacteria of human beings. Animal-borne

Cited by 7 publications

References 20 publications

Incidence of Helicobacter felis and the Effect of Coinfection with Helicobacter pylori on the Gastric Mucosa in the African Population

Incidence of Helicobacter felis and the Effect of Coinfection with Helicobacter pylori on the Gastric Mucosa in the African Population

The efficacy and safety of long-term Helicobacter species quadruple therapy in asymptomatic cats with naturally acquired infection

Gastric Helicobacter spp. infection in captive neotropical Brazilian feline

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