Gastric Helicobacter spp. infection in captive neotropical Brazilian feline

Abstract

Ten captive neotropical Brazilian feline were submitted to gastroscopic examination and samples of gastric mucosa from fundus, corpus and pyloric antrum were evaluated for the presence of Helicobacter species.Warthin-Starry (WS) staining and PCR assay with species-specific primers and enzymatic cleavage were applied for bacterial detection and identification. Histological lesions were evaluated by haematoxylin and eosin staining. All animals showed normal gross aspect of gastric mucosa. Helicobacter heilmannii was confirmed in 100% of the samples by WS and PCR assay. Mild lymphocytic infiltrate in the lamina propria was observed in eight animals, mainly in the fundus region. Small lymphoid follicles were seen in three animals. No significant association between Helicobacter infection and histological findings was verified.These observations suggest that gastric Helicobacter spp. could be a commensal or a eventual pathogen to captive neotropical feline, and that procedures, way life, and stress level on the shelter apparently had no negative repercussion over the integrity of the stomach.

IDENTIFICAÇÃO DE Helicobacter spp. EM MUCOSAS GÁSTRICA E DUODENAL DE CÃES (Canis familiaris) UTILIZANDO A TÉCNICA DE WARTHIN-STARRY

“…Já em região de antro pilórico foi observado que 11,5% (3/26) das amostras apresentavam a bactéria. A existência de bactérias espiraladas na mucosa gástrica pode não estar correlacionada com doenças digestivas do animal (19,20) , podendo, entretanto, ser uma fonte de disseminação dessas bactérias. A falta de sinais clínicos e lesões macroscópicas evidentes em caninos infectados nos levam a crer que a bactéria não seja tão patogênica para esses como são para os felinos, em que as lesões se assemelham às observadas em humanos.…”

Microbial Diversity: Relevance and Relationship Between Environmental Conservation And Human Health

“…and Proteus sp. that assist in the synthesis of degradation of the food (Camargo et al 2011;Odonkor and Ampofo 2013). The intestinal microbiota is a dynamic mixture of microorganisms, whose composition varies along the gastrointestinal tract (GIT) and between the mucosa and the intestinal lumen.…”