Genetic diversity in Brazilian populations of Aedes albopictus

Ayres, Constância Flávia Junqueira; Romão, Tatiany Patrícia; Melo-Santos, Maria Alice Varjal de; Furtado, André Freire

doi:10.1590/s0074-02762002000600022

Cited by 49 publications

(42 citation statements)

References 14 publications

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“…This pattern was also observed using a random amplified polymorphic DNA technique, which showed the high genetic variation observed in local Brazilian populations. 46 One explanation could be that drastic reductions occurred in founding populations that are likely due to insecticide treatments. 47 Over distances of 90−250 km, populations along the northeastern coast of Mexico were isolated by distance.…”

Section: Discussionmentioning

confidence: 99%

Large Genetic Differentiation and Low Variation in Vector Competence for Dengue and Yellow Fever Viruses of Aedes Albopictus From Brazil, the United States, and the Cayman Islands

Oliveira¹,

Vazeille²,

Filippis³

et al. 2003

The American Journal of Tropical Medicine and Hygiene

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Abstract. We conducted a population genetic analysis of Aedes albopictus collected from 20 sites in Brazil, the United States (Florida, Georgia, and Illinois), and the Cayman Islands. Using isoenzyme analysis, we examined genetic diversity and patterns of gene flow. High genetic differentiation was found among Brazilian samples, and between them and North American samples. Regression analysis of genetic differentiation according to geographic distances indicated that Ae. albopictus samples from Florida were genetically isolated by distance. Infection rates with dengue and yellow fever viruses showed greater differences between two Brazilian samples than between the two North American samples or between a Brazilian sample and a North American sample. Introductions and establishments of new Ae. albopictus populations in the Americas are still in progress, shaping population genetic composition and potentially modifying both dengue and yellow fever transmission patterns.

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Section: Discussionmentioning

confidence: 99%

Large Genetic Differentiation and Low Variation in Vector Competence for Dengue and Yellow Fever Viruses of Aedes Albopictus From Brazil, the United States, and the Cayman Islands

Oliveira¹,

Vazeille²,

Filippis³

et al. 2003

The American Journal of Tropical Medicine and Hygiene

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“…Bootstrap analysis was performed with Winboot program (Yap & Nelson 1996) POPGENE genetic software (Yeh & Boyle 1997) was used to determine the percentage of polymorphic loci, heterozigosity, Gst and Nm indices taking into account the same restrictions considered by Ayres et al (2002). Distances among the populations from different sample points were calculated with SPRING software version 3.06.02 (Instituto Nacional de Pesquisas Espaciais, 2002).…”

Section: Methodsmentioning

confidence: 99%

Genetic differentiation among Brazilian populations of Euschistus heros (Fabricius) (Heteroptera: Pentatomidae) based on RAPD analysis

Sosa-Gómez¹,

Delpin²,

Almeida³

et al. 2004

Neotrop. Entomol.

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PALAVRAS-CHAVE: RAPD, marcador molecular, estrutura genética, fluxo gênico ABSTRACT -The Neotropical Brown Stink Bug (NBSB), Euschistus heros (Fabricius), has a wide distribution in Brazilian soybean fields, being more important in the central region of the country. The species is the main target of insecticide applications for stink bug pest control. We determined the variability among and within populations of the NBSB by random amplification of polymorphic DNA (RAPD) analysis. Samples of NBSB were collected in soybean fields from Ubiratã (PR), Londrina (PR), Centenário do Sul (PR), Cândido Mota (SP), Ponta Porã (MS) and Sapezal (MT). Genomic DNA was extracted from the head to minimize DNA contamination by endoparasites. DNA was amplified with 10 mer primers. Fifteen primers produced 246 bands. The genetic similarity matrix was obtained, based on RAPD allele frequencies, using Nei´s 1972 genetic distance. NSBS collected from the same geographical region clustered together. The populations from Londrina and Centenário do Sul were genetically closer than the others and stink bugs collected in Cândido Mota were closer to Ponta Porã population. The Sapezal geographical population was the most divergent from the others. Females and males clustered separately inside each geographical population, implying that RAPD permits gender discrimination. We did not observe individuals from one region clustering together with stink bugs from another region. The number of polymorphic loci from the different populations ranged between 40.6% and 52.1%. The gene flow indexes (overall Nm = 0.8307) were lower than that observed for Anticarsia gemmatalis Hübner and Helicoverpa armigera (Hübner) suggesting that in stink bug populations gene flow is lower than in the noctuid moths.

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“…albopictus Amapá, Roraima, Acre, Tocantins, Piauí and Sergipe (Martins et al 2006). The ability to survive in such different environmental conditions is likely to be infl uenced by differences in the strain populations (Ayres et al 2002). This hypothesis is reinforced by the results from experimental trails performed in the fi eld and laboratory conditions where development of Ae.…”

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confidence: 99%

Eclosion rate, development and survivorship of Aedes albopictus (Skuse)(Diptera: Culicidae) under different water temperatures

Monteiro¹,

Souza²,

Albuquerque³

2007

Neotrop. entomol.

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Neotropical Entomology 36 (6):966-971 (2007) Eclosão, Desenvolvimento e Sobrevivência de Aedes albopictus (Skuse) (Diptera: Culicidae) sob Diferentes Condições de Temperatura da Água RESUMO -Em áreas tropicais, onde as populações de insetos vetores são particularmente abundantes, as temperatura usualmente variam entre 25°C e 35°C. Considerando a importância dessa variação na determinação da dinâmica populacional de mosquitos, neste trabalho, desenvolvimento e as taxas de eclosão e sobrevivência dos estágios imaturos de Aedes albopictus (Skuse) foram comparados sob temperaturas constantes de 25, 30 e 35°C (em câmaras climatizadas) e ambientes (25°C a 29°C). A taxa de eclosão foi considerada como o total de larvas obtidas após 24h. O período de desenvolvimento, assim como a taxa de sobrevivência larval e pupal foram avaliados diariamente. A taxa de eclosão foi signifi cativamente mais elevada sob temperatura ambiente comparada às constantes, sugerindo que a variação da temperatura pode ser um fator estimulante da eclosão. O tempo médio de eclosão aumentou com a temperatura, variando de 2,8h (25ºC) a 5,2h (35ºC). A duração do período larval apresentou grande variabilidade dentro de cada grupo, embora não tenha diferido signifi cativamente entre os mesmos (11,0 ± 4,19 dias), tendo sido mais longo para indivíduos mantidos na água a 35°C (12,0 ± 4,95 dias) e temperatura ambiente (13,6 ± 5,98 dias). Ao contrário, a sobrevivência das larvas foi fortemente afetada na temperatura mais elevada, onde apenas um indivíduo alcançou o estágio adulto. Esses resultados sugerem que a população de Ae. albopictus de Recife pode estar em processo de adaptação ao aumento de temperatura e que o limite para o desenvolvimento de estágios larvais se encontra próximo a 35°C. PALAVRAS-CHAVE: Biologia, vetor, ciclo de vida, limite térmicoABSTRACT -In tropical areas, where vector insects populations are particularly numerous, temperature usually range between 25°C and 35°C. Considering the importance of such temperature variation in determining mosquitoes population dynamics, in this work the developmental, eclosion and survival rates of the immature stages of Aedes albopictus (Skuse) were compared under constant 25, 30 and 35°C (using acclimatized chambers) and environmental (25°C to 29°C) temperatures. The hatching rate was considered as total number of larvae recovered after 24h. The development period as well as larval and pupal survival rate were evaluated daily. Eclosion rate was signifi cantly higher under environmental temperature than under the studied constant temperatures, suggesting that temperature variation may be an eclosion-stimulating factor. The mean eclosion time increased with the temperature, ranging from 2.8h (25°C) to 5.2h (35°C). The larval period was greatly variable inside each group, although it did not differ signifi cantly amongst groups (11.0 ± 4.19 days), with individuals showing longer larval stages in water at 35°C (12.0 ± 4.95 days) and environmental temperature (13.6 ± 5.98 days). Oppositely, survival was...

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Genetic diversity in Brazilian populations of Aedes albopictus

Abstract: Random amplified polymorphic DNA (RAPD) analysis technique was undertaken in

Cited by 49 publications

References 14 publications

Large Genetic Differentiation and Low Variation in Vector Competence for Dengue and Yellow Fever Viruses of Aedes Albopictus From Brazil, the United States, and the Cayman Islands

Large Genetic Differentiation and Low Variation in Vector Competence for Dengue and Yellow Fever Viruses of Aedes Albopictus From Brazil, the United States, and the Cayman Islands

Genetic differentiation among Brazilian populations of Euschistus heros (Fabricius) (Heteroptera: Pentatomidae) based on RAPD analysis

Eclosion rate, development and survivorship of Aedes albopictus (Skuse)(Diptera: Culicidae) under different water temperatures

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