Genetic differentiation among Brazilian populations of Euschistus heros (Fabricius) (Heteroptera: Pentatomidae) based on RAPD analysis

Sosa-Gómez, Daniel Ricardo; Delpin, Katiaíres E.; Almeida, A. M. R.; Hirose, Edson

doi:10.1590/s1519-566x2004000200009

Cited by 19 publications

(19 citation statements)

References 9 publications

(10 reference statements)

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“…With few exceptions, pupae of the same sex clustered together in the Planaltina, Londrina and Passo Fundo populations. Although similar results have been obtained for the neotropical brown stink bug, Euschistus heros (Fabricius 1798) (Sosa-Gómez et al, 2004) and for Triatoma brasiliensis (Neiva, 1911), sex-clustering was not reported by Borges et al, (2000). A. gemmatalis larvae collected from peanut fields in Marianna, and on soybean in Quincy, (70 km apart) were similar.…”

Section: Resultssupporting

confidence: 75%

Intraspecific variation and population structure of the Velvetbean Caterpillar, Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae)

Sosa–Gómez

2004

Genet. Mol. Biol.

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The velvetbean caterpillar (VBC), Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae), is one of the most important New World soybean agro-ecosystems pests, occurring from 40°N in the USA to 39°S in Argentina. Information on the migration patterns of the VBC moth may be important for managing the resistance of VBC populations to insecticides or plants carrying the Bacillus thuringiensis insecticide genes, especially since sedentary populations have a higher potential to became resistant than migratory populations. We studied intraspecific variations of geographically distinct VBC populations in order to determine the genetic distance between them and to assess the variability of VBC populations from near the city of Londrina (Paraná (PR) state, Brazil). Samples of the VBC were obtained from sites near the following towns or cities: Marianna and Quincy (Florida, USA); La Virginia (Tucumán province, Argentina); Londrina (PR), Passo Fundo (Rio Grande do Sul, Brazil) and Planaltina (Goiás, Brazil). The VBC samples were used to construct a genetic similarity matrix based on random amplified polymorphic DNA (RAPD) allele frequencies, the cotton leafworm, Alabama argillacea, Hübner 1823 (Insecta: Lepidoptera: Noctuidae), being used as an outgroup. Interestingly, despite the great distance (about 6,500 km) between Planaltina and Quincy some of the specimens from the Quincy population clustered in a group genetically close to the Planaltina populations. Larvae collected on peanuts in Marianna and on soybean in Quincy, 70 km apart, appeared genetically similar. The population from Planaltina was the most heterogeneous (polymorphism = 85.6%; heterozygosity = 0.1505). The Argentinean VBC population was entirely different from the Brazilian populations. The genetic similarities found between individuals from geographically distant populations and effective migration rate values (2.0566 > Nm < 15.2618) indicate that migration occurs.

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Section: Resultssupporting

confidence: 75%

Intraspecific variation and population structure of the Velvetbean Caterpillar, Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae)

Sosa–Gómez

2004

Genet. Mol. Biol.

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“…Genetic studies using RAPD markers of individuals of this species collected during 2000 (Sosa‐Gómez et al., ) revealed genetic differentiation among E. heros geographic populations. However, some aspects should be considered to explain differences between these findings and the results of the present study.…”

Section: Discussionmentioning

confidence: 99%

Structure and genetic variation among populations of Euschistus heros from different geographic regions in Brazil

Husch

Ferreira

Seraphim

et al. 2018

Entomologia Exp Applicata

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The Neotropical brown stink bug, Euschistus heros (Fabricius) (Hemiptera: Pentatomidae), is a major Brazilian soybean pest. Aiming to provide relevant information to implement pest and insecticide resistance management, new microsatellite loci were developed for E. heros and used in a study of genetic diversity and population structure. The population analysis was performed using eight microsatellite loci from 17 samples (n = 243 individuals) collected in the major soybean‐producing regions in Brazil (northeastern, midwestern, and southern regions). These microsatellite loci provided high genetic diversity values on the whole extension for the studied region (He = 0.895; total number of alleles = 400). Neotropical brown stink bug populations in general displayed low genetic structure levels among the samples (overall ΦST = 0.009). An exception was the sample from the northeastern region, which showed a significant genetic differentiation (pairwise ΦST = 0.031–0.063). Bayesian cluster analysis confirmed these results, did not show population subdivision, and indicated considerable levels of gene flow. Significant correlations between genetic differences and geographic distance were obtained. The lowest estimate of migration was found in the population from São Desidério, which was also the most distant from the remaining populations based on genetic distance. Some plausible hypotheses for the low genetic differentiation among these populations are the fast expansion of soybean production areas, the main food source of E. heros, polyvoltinism, and possible influence of anthropogenic dispersal. All these factors could have led to high population densities, a wide distribution that may contribute to reduced population differentiation, and increased genetic diversity.

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“…These variations among geographically isolated S. frugiperda indicate the presence of factors that affect the genetic variability and selection of biotypes in the two analyzed crops. As recommended by Sosa-Gomez et al (2004), in order to validate these results, it will be necessary to make ecological observations or characterizations of, for example, the insect's flying behavior, dispersion ability, and resistance to chemical or biological products. Despite this, the mitochondrial ND1 gene showed high potential to discriminate these two biotypes of S. frugiperda, being a quick method to identify these pests.…”

Section: Marcadores Mitocondriais Para Diferenciar Populações De Spodmentioning

confidence: 99%

Mitochondrial markers to distinguish Spodoptera frugiperda populations associated with corn and cotton crops

Queiroz¹,

Ramiro

Martins

et al. 2016

Pesq. agropec. bras.

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-The objective of this work was to analyze the genetic variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) populations collected from corn and cotton crops in Brazil. The samples were analyzed by DNA markers. The dendrogram produced by the 20 RAPD primers evaluated showed a correlation between genetic profile and feeding behavior. The analysis of the mitochondrial ND1 gene allowed identifying the insect populations in both crops, and, in corn, in several geographical regions. The presented strategy allows the identification of Spodoptera frugiperda populations associated with corn and cotton crops.Index terms: Gossypium hirsutum, Zea mays, fall armyworm, haplotypes, ND1 gene, RAPD. Marcadores mitocondriais para diferenciar populações de Spodoptera frugiperda associadas às culturas de milho e algodãoResumo -O objetivo deste trabalho foi avaliar a variabilidade genética de populações de Spodoptera frugiperda (Lepidoptera: Noctuidae) coletadas nas culturas de milho e algodão do Brasil. As amostras foram analisadas por marcadores de DNA. O dendrograma produzido pelos 20 iniciadores de RAPD avaliados revelou correlação entre o perfil genético e o comportamento de alimentação. A análise do gene ND1 mitocondrial permitiu identificar populações do inseto em ambas as culturas, e, em milho, em várias regiões geográficas. A estratégia apresentada permite a identificação de populações de Spodoptera frugiperda associadas às culturas de milho e algodão.Termos para indexação: Gossypium hirsutum, Zea mays, lagarta-do-cartucho, haplótipos, gene ND1, RAPD.Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae), usually known as fall armyworm, has a polyphagous feeding behavior and is a serious pest of 80 plant species, including cotton (Gossypium hirsutum L.), corn (Zea mays L.), and soybean (Glycine max L.) (Martinelli et al., 2006;Barros et al., 2010).Initial studies of S. frugiperda populations from North American and Caribbean countries showed the existence of two strains: the corn strain, associated with corn and sorghum (Sorghum bicolor L.); and the rice strain, found preferentially in rice (Oryza sativa L.) and turfgrassess. These strains differ in their host preference, physiology, behavior, and pesticide susceptibility (Prowell et al., 2004). However, both host strains can only be reliably distinguished by biochemical and molecular markers, which remain the most accurate indicators of strain identity worldwide (Nagoshi & Meagher, 2008). Busato et al. (2004) and Pinto et al. (2015) reported genetic variability among S. frugiperda populations associated with cotton and corn in Brazil, using amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers. marker based on the NADH dehydrogenase 1 (ND1) gene was adopted; this gene has been previously used for phylogenetic analyses of different lepidopteran species, including S. frugiperda (Pashley & Ke, 1992). The objective of this work was to analyze the genetic variability of S. frugiperda populations collected...

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Genetic differentiation among Brazilian populations of Euschistus heros (Fabricius) (Heteroptera: Pentatomidae) based on RAPD analysis

Cited by 19 publications

References 9 publications

Intraspecific variation and population structure of the Velvetbean Caterpillar, Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae)

Intraspecific variation and population structure of the Velvetbean Caterpillar, Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae)

Structure and genetic variation among populations of Euschistus heros from different geographic regions in Brazil

Mitochondrial markers to distinguish Spodoptera frugiperda populations associated with corn and cotton crops

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