Phenotypic and molecular characterization of antimicrobial resistance and virulence factors in Pseudomonas aeruginosa clinical isolates from Recife, State of Pernambuco, Brazil

Jácome, Paula Regina Luna de Araújo; Alves, Lílian Rodrigues; Cabral, Adriane Borges; Lópes, Ana Catarina de Souza; Maciel, Maria Amélia Vieira

doi:10.1590/s0037-86822012000600010

Cited by 30 publications

(21 citation statements)

References 46 publications

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“…13 . Brezilya'da yapılan bir çalışmada ise, yatan hastalardan izole edilen P.aeruginosa suşlarında direnç oranları AMK, GEN, CIP, aztreonam (ATM), sefepim (FEP), CAZ, IPM ve meropenem (MEM) için sırasıyla; %34, %41, %41, %29, %34, %32, %32 ve %37 olarak bulunmuştur 14 . Özyurt ve arkadaşları 15 2010 yılında İstanbul bölgesinde yaptıkları çalışmada, AMK, GEN, levofl oksasin (LVX), CIP, CAZ, FEP, ATM, IPM ve MEM'e sırasıy-la; %17.6, %44.9, %21.2, %20, %64.3, %45.1, %60.3, %22.9 ve %18.9 oranlarında direnç saptamışlardır.…”

Section: Discussionunclassified

Molecular Epidemiology of Beta-Lactamases in Ceftazidime-Resistant Pseudomonas aeruginosa Isolates

Er¹,

Altındiş²,

Aşık³

et al. 2015

Mikrobiyol Bul

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ÖZPseudomonas aureginosa, özellikle immün sistemin baskılandığı hastalarda, yaşlılarda ve ağır yanık durumlarında hastalık oluşturan ve daha çok hastane enfeksiyonlarına neden olabilen önemli bir fırsatçı patojendir. Bakterinin birçok antibiyotiğe karşı yüksek oranda direnç geliştirme özelliği, P.aeruginosa enfeksiyonlarının mortalite ve morbiditesini artırmaktadır. Bu çalışmada, yatan hastalardan izole edilen P.aeruginosa suşlarının antibiyotik duyarlılıklarının belirlenmesi ve PER, GES, KPC, VIM, IMP ve OXA gibi direnç enzimlerinin varlığının araştırılması amaçlanmıştır. Çalışmaya, 2010-2012 yılları arasında Afyon Kocatepe Üniversitesi Tıp Fakültesi Hastanesi'nde yatan 134'ü erkek 61'i kadın hastanın çeşitli klinik örneklerinden (29 balgam, 67 yara, 53 trakeal aspirat, 23 kan, 18 idrar, 3 beyin omurilik sıvısı, 2 plevral sıvı) izole edilen, 195 P.aeruginosa suşu dahil edilmiştir. İzolatların tanımlanmasında konvansiyonel ve otomatize sistemler (VITEK 2, BioMerieux, Fransa) kullanılmış; antibiyotik duyarlılıklarının belirlenmesi için disk difüzyon ve E-test yöntemleri uygulanmıştır. İzolatların indüklenebilir beta-laktamaz (İBL), genişlemiş spektrumlu beta-laktamaz (GSBL) ve metallo-beta-laktamaz (MBL) üretimleri, fenotipik olarak, sırasıyla çift disk indüksiyon yöntemi, çift disk sinerji testi ve E-test yöntemi ile saptanmıştır. İzolatlarda direnç enzimlerini (PER, GES, KPC, VIM, IMP ve OXA) kodlayan genlerin varlığı ise gerçek zamanlı polimeraz zincir reaksiyonu ile araştırılmış; pozitif örneklere dizi analizi uygulanmıştır. Çalışmamızda, 195 P.aeruginosa suşunun tümü (%100) seftazidime, %90.8'i tazobaktam/piperasiline, %60.5'i aztroenama, %50.2'si sefepime, %48.2'si imipeneme, %47.2'si meropeneme, %47.2'si ofl oksasine, %44.1'i pipeGeliş Tarihi

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Section: Discussionunclassified

Molecular Epidemiology of Beta-Lactamases in Ceftazidime-Resistant Pseudomonas aeruginosa Isolates

Er¹,

Altındiş²,

Aşık³

et al. 2015

Mikrobiyol Bul

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“…In the same concern, Minhas et al (2015) reported that 90% of all isolates showed gelatinase activity and 60% were hemolysin evidenced, by hemolysis on blood agar, 70% were found to express protease activity, while 66.67% and 83.33% were biofilm producers. Jácome et al (2012) indicated that 93.4%, 72.1% and 34.4% of P. aeruginosa strains having gelatinase, hemolysin and biofilm production activities, respectively. P. aeruginosa also produces some proteases (LasB elastase, LasA elastase and alkaline protease) which are able to destroy the protein elastin.…”

Section: Phenotypic Expression Of Virulence Factorsmentioning

confidence: 99%

Antibacterial Potential of a Newly Synthesized Zinc Peroxide Nanoparticles (Zno2-Nps) to Combat Biofilm-Producing Multi-Drug Resistant Pseudomonas aeruginosa

et al. 2019

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Introduction Multidrug-resistant (MDR) bacteria remain the greatest challenge in public health care. The numbers of infections produced by such resistant strains are increasing globally. This acquired resistance of pathogens presents a key challenge for many antimicrobial drugs (Ali et al., 2019). Such multidrug-resistant (MDR) microbes make the treatment more difficult and expensive with more side effects. Many diseases showed difficulties in their treatment after the advent of MDR bacteria

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“…P. aeruginosa has different mechanisms of resistance, which generates multi-drug resistant isolates or panresistant isolates (Jácome et al, 2012). Several mechanisms of resistance which the pathogen develops include multidrug efflux pump, production of βlactamases and aminoglycoside modifying enzymes (Mahmoud et al, 2013) which reflect the increasing rate of multidrug resistant strains worldwide causing a serious problem in hospital settings (Lim et al, 2009).…”

Section: Sourcementioning

confidence: 99%

Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals

Raafat¹,

Ali-Tammam²,

Ali³

2016

Afr. J. Microbiol. Res.

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Aim & Objectives: The present study was aimed to determine phenotypic characterization of virulent Pseudomonas aeruginosa and its Type 3 Secretion system from Surat region. Methods: 125 clinical Pseudomonas aeruginosa were procured from different laboratories of Surat and 53 environment Pseudomonas aeruginosa isolated from 125 various environment and vegetable samples. Antimicrobial susceptibility testing was performed by Kirby Bauer Disc Diffusion method. The phenotypic screening evaluated for six soluble virulence factors (amylase, Caseinase, gelatinase, haemolysins, lecithinase, and lipase). Biofilm formation was detected by Tube method and Congo red agar method. Four virulence genes (exoS, exoT, exoU, exoY) were screened in selected isolates by PCR. Results: A high percentage of resistance rates (89.60% to amoxyclav and 88% to Cefuroxime) were observed amongst most of the isolates. Soluble enzymes were expressed in variable proportions (Gelatinase 96.80%, Lipase 96%, Lecithinase 88%, and Caseinase 88%). Tube method was considered to be superior to congo red agar method. The most frequent virulent gene detected was exoU (100%). Conclusions: Clinical and environmental virulent Pseudomonas aeruginosa harbour various genes that represent type 3 secretary systems and have high level of resistance that may result in to therapeutic failure.

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Phenotypic and molecular characterization of antimicrobial resistance and virulence factors in Pseudomonas aeruginosa clinical isolates from Recife, State of Pernambuco, Brazil

Cited by 30 publications

References 46 publications

Molecular Epidemiology of Beta-Lactamases in Ceftazidime-Resistant Pseudomonas aeruginosa Isolates

Molecular Epidemiology of Beta-Lactamases in Ceftazidime-Resistant Pseudomonas aeruginosa Isolates

Antibacterial Potential of a Newly Synthesized Zinc Peroxide Nanoparticles (Zno2-Nps) to Combat Biofilm-Producing Multi-Drug Resistant Pseudomonas aeruginosa

Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals

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