Phenotypic and genotypic characterization of Pseudomonas aeruginosa isolates from Egyptian hospitals

Abstract

Aim & Objectives: The present study was aimed to determine phenotypic characterization of virulent Pseudomonas aeruginosa and its Type 3 Secretion system from Surat region. Methods: 125 clinical Pseudomonas aeruginosa were procured from different laboratories of Surat and 53 environment Pseudomonas aeruginosa isolated from 125 various environment and vegetable samples. Antimicrobial susceptibility testing was performed by Kirby Bauer Disc Diffusion method. The phenotypic screening evaluated for six soluble virulence factors (amylase, Caseinase, gelatinase, haemolysins, lecithinase, and lipase). Biofilm formation was detected by Tube method and Congo red agar method. Four virulence genes (exoS, exoT, exoU, exoY) were screened in selected isolates by PCR. Results: A high percentage of resistance rates (89.60% to amoxyclav and 88% to Cefuroxime) were observed amongst most of the isolates. Soluble enzymes were expressed in variable proportions (Gelatinase 96.80%, Lipase 96%, Lecithinase 88%, and Caseinase 88%). Tube method was considered to be superior to congo red agar method. The most frequent virulent gene detected was exoU (100%). Conclusions: Clinical and environmental virulent Pseudomonas aeruginosa harbour various genes that represent type 3 secretary systems and have high level of resistance that may result in to therapeutic failure.

Prevalence of Quorum Sensing and Virulence Factor Genes Among Pseudomonas aeruginosa Isolated from Patients Suffering from Different Infections and Their Association with Antimicrobial Resistance

“…Different mechanisms of P. aeruginosa resistance result in the emergence of isolates that are pan-drug-resistant (PDR) or multi-drug resistant (MDR). 8 The pathogen exhibits a variety of these mechanisms such as multidrug efflux pump, β-lactamases, and aminoglycoside modifying enzymes 9 that increase the chance of developing multidrug resistant strains representing a significant threat to health-care facilities and created increasing immense clinical global problem. 10 Bacterial resistance syndrome is a property of P. aeruginosa , as this strain possesses almost all known antimicrobial resistance mechanisms.…”

<p>Quorum quenching activity of <em>Bacillus cereus</em> isolate 30b confers antipathogenic effects in <em>Pseudomonas aeruginosa</em></p>

“…A total of 33 clinical isolates of P. aeruginosa previously identified with API 20NE System (Biomerieux, France) were included in this study. They were selected from our culture collection based on the presence of one or more virulence factors, as described previously 45. (Table S1).…”