Detecção de metalo-beta-lactamase em Pseudomonas aeruginosa isoladas de pacientes hospitalizados em Goiânia, Estado de Goiás

Gonçalves, Diana Christina Pereira Santos; Lima, Ana Beatriz Mori; Leão, Lara Stefânia Netto de Oliveira; Filho, José Rodrigues do Carmo; Pimenta, Fabiana Cristina; Vieira, José Daniel Gonçalves

doi:10.1590/s0037-86822009000400010

Cited by 21 publications

(15 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Dice (Tol 1.0%-1.0%) obtained in phenotypic and genotypic tests has also been reported in other studies (13) (14) and may be related to diffi culties in reading and interpreting tests, variations in the sensitivity and specifi city of the method according to the combination of substrate and chelating agent, the concentration of the chelating agent, and the presence of other resistance mechanisms that may affect the results of the phenotypic tests.…”

Section: Rev Soc Bras Med Trop 48(2):212-215 Mar-apr 2015supporting

confidence: 60%

“…Different rates of SPM-producing P. aeruginosa isolates have been reported in Brazil, varying according to geographic region as follows: 7.5% in São Luis (5) (in Northeastern Brazil), 20.7% in Recife (4) (in Northeastern Brazil), and 42% in Goiás (13) (in Mid-west Brazil). The two SPM-1-positive isolates showed electrophoretic profi les indistinguishable from or closely related to the bla SPM-1 -positive control strains (P1088 and 365h2).…”

Section: Rev Soc Bras Med Trop 48(2):212-215 Mar-apr 2015mentioning

confidence: 99%

See 1 more Smart Citation

Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

Costa

Fleming

Paula

et al. 2015

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidimeresistant P. aeruginosa isolates from State of Sergipe, Brazil. Methods: Metallo-β-lactamase was investigated using the disk approximation test and polymerase chain reaction (PCR). Genetic diversity was evaluated by pulsed-fi eld gel electrophoresis (PFGE). Results: A total of 48 (51.6%) isolates were resistant to ceftazidime. Six (12.2%) of these were positive for metallo-β-lactamase production. Only two (4.1%) of the ceftazidime-resistant isolates carried the bla SPM-1 gene. Conclusions: Production of metallo-β-lactamases was not the main mechanism of resistance to ceftazidime and carbapenems among P. aeruginosa strains in Sergipe, Brazil.

show abstract

Section: Rev Soc Bras Med Trop 48(2):212-215 Mar-apr 2015supporting

confidence: 60%

Section: Rev Soc Bras Med Trop 48(2):212-215 Mar-apr 2015mentioning

confidence: 99%

Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

Costa

Fleming

Paula

et al. 2015

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

show abstract

“…Multidrug resistance of NFGNB stems from different factors, such as up-regulated production of enzymes metabolizing the drugs, target site changes, overexpression of efflux pumps, and porin deficiency 5,6 . Metallobeta-lactamase (MBL) production, mainly by Pseudomonas aeruginosa, stands out as a frequent cause of severe nosocomial infections 7,8 . The prevalence, phenotypic characteristics, and antimicrobial susceptibility profile of NFGNB strains may show regional variation.…”

mentioning

confidence: 99%

Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Argenta

Fuentefria

Sobottka

2017

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction:We compared the prevalence and antimicrobial susceptibility of non-fermenting gram-negative bacilli (NFGNB) isolated from clinical samples at a Brazilian tertiary care hospital in 2008 and 2013. Methods: Collected data included patient's name, age, sex, inpatient unit, laboratory record number, type of biological material, culture test result, and antimicrobial susceptibility of isolated strains. Results: Out of 19,112 culture tests analyzed, 926 (4.8%) were positive for NFGNB. Among these, 45.2% were metallo-beta-lactamase (MBL) producing strains. Conclusion: Between 2008 and 2013, the number of MBL-producing NFGNB isolates increased by 21.5%, which was accompanied by a consequent reduction in susceptibility to antimicrobials.

show abstract

“…As an example, one could cite rates of 10.9% in hospitals in Rio Grande do Sul, 24 20% in Rio de Janeiro -RJ, 25 and 41.9% in the state of Goiás. 26 Figueiredo-Mendes et al 27 described higher rates of this enzyme production (77.8%) among P. aeruginosa strains previously resistant to carbapenems in hospitals in São Paulo and Brasília.…”

mentioning

confidence: 95%

Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil

Rodrigues¹,

Chang²,

Nóbrega³

et al. 2011

Braz J Infect Dis

View full text Add to dashboard Cite

Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1 st , 2007 to June 31 st , 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals.

show abstract

Detecção de metalo-beta-lactamase em Pseudomonas aeruginosa isoladas de pacientes hospitalizados em Goiânia, Estado de Goiás

Cited by 21 publications

References 17 publications

Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil

Contact Info

Product

Resources

About