Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Argenta, Anne de Rossi; Fuentefria, Daiane Bopp; Sobottka, Andréa Michel

doi:10.1590/0037-8682-0371-2016

Cited by 2 publications

(5 citation statements)

References 11 publications

(13 reference statements)

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“…It seems that the high pressure of antibiotics due to greater empirical or indiscriminate use of antibiotics can lead to carbapenem resistance in the hospital setting. Moreover, consistent with our study, a large outbreak of MBL-producing NFGNB infections, especially due to A. baumannii isolates were described in India ( 8 , 9 ), Brazil ( 1 ), Pakistan ( 32 ), and Nepal ( 33 ). In the present study, it was found that 33.3% and 46.7% of carbapenem-resistant P. aeruginosa isolates, and 13.3% and 28.9% of carbapenem-resistant A. baumannii isolates were harboring bla IMP-1 and bla VIM-1 genes, respectively.…”

Section: Discussionsupporting

confidence: 91%

“…Non-fermentative Gram-negative bacilli have emerged as a significant cause of healthcare-associated infections. The ever-increasing emergence of antibiotic resistance in NFGNB and the lack of new antibacterial agents is a major challenge for treating patients suffering from infections caused by them ( 1 , 23 ).…”

Section: Discussionmentioning

confidence: 99%

“…Another study found that MBL positive isolates of A. baumannii were showing significantly higher resistance to all antimicrobials agents as compared to MBL negative isolates ( 8 ). In the study of Argenta and colleagues (2017), high levels of antibiotic resistance were reported in MBL-producing NFGNB isolates compared to Non-MBL isolates ( 1 ). In a previous study, all MBL-producing P. aeruginosa was isolated from In-patients only ( 10 ).…”

Section: Discussionmentioning

confidence: 99%

“…Non-fermentative Gram-negative bacilli including Pseudomonas aeruginosa, Acinetobacter baumannii, and Stenotrophomonas maltophilia are opportunistic pathogens with a highly variable level of virulence that rarely causes disease in healthy individuals. However, these bacteria as one of the most important nosocomial pathogens can cause severe infections such as bacteremia, urinary tract and surgical site infections in hospitalized and immunocompromised patients ( 1 – 3 ).…”

Section: Introductionmentioning

confidence: 99%

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High prevalence of multidrug-resistant non-fermentative Gram-negative bacilli harboring bla and bla metallo-beta-lactamase genes in Birjand, south-east Iran

Namaei¹,

Yousefi²,

Askari³

et al. 2021

IJM

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Background and Objectives: Non-fermentative Gram-negative Bacilli (NFGNB) is known as a major cause of health- care-associated infections with high levels of antibiotic resistance. The aim of this study was to investigate the antibiotic resistance profiles and molecular characteristics of metallo-beta-lactamase (MBL)-producing NFGNB. Materials and Methods: In this cross-sectional study, the antibiotic resistance profile of 122 clinical NFGNB isolates was determined by the Kirby-Bauer disk diffusion and microdilution broth methods. Bacterial isolates were investigated for the detection of MBLs production using the combination disk diffusion Test (CDDT). The existence of bla , bla , and bla NDM genes in all carbapenem-resistant isolates was determined employing polymerase chain reaction (PCR) assays. Results: High resistance in Pseudomonas aeruginosa was reported to cefotaxime and minocycline, whereas Acinetobacter baumannii isolates were highly resistant to all antibiotics except colistin. Multidrug resistance (MDR)-NFGNB (66% vs. 12.5%, P=0.0004) and extensively drug resistant (XDR)-NFGNB (55.7% vs. 12.5%, P=0.001) isolates were significantly more common in hospitalized patients than in outpatients. The production of MBL was seen in 40% of P. aeruginosa and 93.3% of A. baumannii isolates. It was found that 33.3% and 46.7% of carbapenem-resistant P. aeruginosa isolates, and 13.3% and 28.9% of carbapenem-resistant A. baumannii isolates were harboring bla IMP-1 and bla VIM-1 genes, respectively. The incidence of MDR (98.2% vs. 28.3%, P<0.001) and XDR (96.4% vs. 11.7%, P<0.001) in MBL-producing NFGNB isolates was significantly higher than non-MBL-producing isolates. Conclusion: This study demonstrated a higher rate of resistance among NFGNB isolates with an additional burden of MBL production within them, warranting a need for robust microbiological surveillance and accurate detection of MBL producers among the NFGNB.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High prevalence of multidrug-resistant non-fermentative Gram-negative bacilli harboring bla and bla metallo-beta-lactamase genes in Birjand, south-east Iran

Namaei¹,

Yousefi²,

Askari³

et al. 2021

IJM

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“…Alterations of chromosomal genes are still by far the most critical mechanisms of β-lactam resistance in NFGNBs, although transferable carbapenem resistance is becoming increasingly important 1 .…”

Section: Discussionmentioning

confidence: 99%

Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran

Porbaran¹,

Habibipour²

2020

J Pure Appl Microbiol

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Bacteria are capable of evolving high doses of the drug in various infections by forming biofilms. Perhaps, biofilm regulator genes have different frequencies in β-lactam producing non-fermentative Gram-negative Bacilli (NFGNB). In this study, we investigated the role of biofilm operons of Pseudomonas aeruginosa and Acinetobacter baumannii on the prevalence of different β-lactamase enzymes. One-hundred twenty (120) nosocomial NFGNB isolates were collected from different clinical samples of patients. PCR method was used for the amplification of resistance genes. Isolates were collected, including 50 isolates (41.66%) of P. aeruginosa and 70 isolates (58.33%) of A. baumannii. The distribution of ESBL, AmpC, KPC, and MBL β-lactamase enzymes in P. aeruginosa and A. baumannii isolates were 64%, 58%, 38%,44%, and 57.14%, 60%, 32.85%, 34.28%, respectively. The frequency of csuABC, pgaABC operon in A. baumannii were as follows: pgaA (45.71%), pgaB (32.85%), pgaC (42.85%), csuA (34.28%), csuB (32.85%), csuC (41.42%), and ompA (38.57%). Further, the prevalence of pslABC and pelABC operons in P. aeruginosa isolates were as follows: pslA (58%), pslB (58%), pslD (60%), pelA (64%), pelB (38%), pelC (44%), and algD (68%). This study revealed that the abundance of biofilm regulator genes in NFGNB strains is affected by different β-lactamase enzymes.

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Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital

Cited by 2 publications

References 11 publications

High prevalence of multidrug-resistant non-fermentative Gram-negative bacilli harboring bla and bla metallo-beta-lactamase genes in Birjand, south-east Iran

High prevalence of multidrug-resistant non-fermentative Gram-negative bacilli harboring bla and bla metallo-beta-lactamase genes in Birjand, south-east Iran

Relationship between Biofilm Regulating Operons and Various Β-Lactamase Enzymes: Analysis of the Clinical Features of Infections caused by Non-Fermentative Gram-Negative Bacilli (Nfgnb) from Iran

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