Observações sobre Blastocystis hominis e Cyclospora cayetanensis em exames parasitológicos efetuados rotineiramente

Alarcón, Ruth Semira Rodríguez; Neto, Vicente Amato; Gakiya, Érika; Bezerra, Rita Cristina

doi:10.1590/s0037-86822007000200024

Cited by 8 publications

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“…identification can be explained by the polymorphism of this protozoan, with different evolutionary forms (vacuolar, granular, amoeboid and cystic), and a wide range of sizes, varying from 2 to 200 micrometers (13)(14)(15). Moreover, the hypertonic zinc sulphate solution may not be appropriate for the detection of Blastocystis spp., due to the possibility of parasite membrane lysis (16,17).…”

Section: Discussionmentioning

confidence: 99%

Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites

et al. 2016

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Introduction:The diagnosis of intestinal parasitic infections depends on the parasite load, the specific gravity density of the parasite eggs, oocysts or cysts, and the density and viscosity of flotation or sedimentation medium where faeces are processed. Objective: To evaluate the concordance between zinc sulphate flotation and centrifugal sedimentation in the recovery of parasites in faecal samples of children. Materials and methods: Faecal samples of 330 children from day care centers were evaluated by zinc sulphate flotation and centrifugal sedimentation techniques. The frequencies of detection of parasites by each method were determined and the agreement between the diagnostic techniques was evaluated using the kappa index, with 95% confidence intervals. Results: The faecal flotation in zinc sulphate diagnosed significantly more cases of Trichuris trichiura infection when compared to centrifugal sedimentation (39/330; 11.8% vs. 13/330; 3.9%, p<0.001), with low diagnostic concordance between methods (kappa=0.264; 95% CI: 0.102-0.427). Moreover, all positive samples for Enterobius vermicularis eggs (n=5) and Strongyloides stercoralis larvae (n=3) were diagnosed only by zinc sulphate. No statistical differences were observed between methods for protozoa identification. Conclusions:The results showed that centrifugal flotation in zinc sulphate solution was significantly more likely to detect light helminths eggs such as those of T. trichiura and E. vermicularis in faeces than the centrifugal sedimentation process.

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Section: Discussionmentioning

confidence: 99%

Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites

et al. 2016

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“…DIScUSSãO Vários estudos sobre a importância do Blastocystis hominis em patologias humanas têm sido realizados 1,3,6,8,9,12,14,23 . Entretanto, seu papel exato ainda não se encontra perfeitamente elucidado.…”

Section: Rev Soc Bras Med Trop 43(3):309-312 Mai-jun 2010unclassified

“…No Brasil, sua presença já foi relatada em diversos estudos, seja em portadores hígidos ou em imunodeprimidos 1,7,[8][9][10][11] .…”

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“…Vários estudos indicam que os métodos de sedimentação espontânea ou de flutuação são ineficazes na busca de Blastocystis hominis nas fezes, pois o contato deste protozoário com água acarretaria a lise do mesmo, produzindo resultados falsonegativos [9][10][11]17 . Uma alternativa para melhorar o desempenho destas técnicas parece ser a conservação prévia das mesmas com formol ou outro tipo de conservante 18 .…”

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“…A grande maioria dos autores encontra consenso quando afirma que a dificuldade na identificação deste protozoário está no treinamento adequado dos analistas clínicos, que possuem dificuldade em reconhecer e identificar o Blastocystis hominis ou, ainda, não atribuem a devida importância ao mesmo, não o mencionando nos resultados dos exames 7,9,10,17 .…”

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Padronização do diagnóstico de Blastocystis hominis por diferentes técnicas de coloração

Eymael

Schuh

Tavares

2010

Rev. Soc. Bras. Med. Trop.

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INTRODUÇÃO: O presente trabalho foi realizado no período de março a maio de 2008, com o objetivo de avaliar a eficácia de diferentes técnicas para diagnóstico de Blastocystis hominis em uma amostra da população atendida pelo Laboratório de Biomedicina da Universidade Feevale, Novo Hamburgo/RS. MÉTODOS: Foram estudadas 100 amostras de fezes de crianças e adultos, que foram coletadas e submetidas às técnicas de sedimentação espontânea (HPJ), sedimentação em formol-éter (Ritchie) e de coloração por Gram e May-Grünwald-Giemsa (MGG). RESULTADOS: A presença de Blastocystis hominis foi observada em 40 amostras, quando utilizadas técnicas de coloração (MGG e Gram), enquanto que as técnicas de sedimentação se mostraram menos eficientes (32 amostras positivas para a técnica de Ritchie e 20 amostras positivas para a técnica de HPJ). CONCLUSÕES: Nossos resultados demonstram que o HPJ foi menos eficiente que outros métodos, indicando a necessidade de se incluir na rotina do laboratório técnicas que permitam a identificação deste parasita.

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Evaluation of the effect of 1,3-bis-(4-phenyl-[1,2,3] triazole-1-il)2-propanol in comparison with metronidazole in an in vitro culture of Blastocystis in samples of patients with irritable bowel syndrome

et al. 2019

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Metronidazole is the most-used pharmaceutical for the treatment of infection by Blastocystis. However, studies have reported resistance of the microorganism towards this pharmaceutical. In Mexico, studies concerning the prevalence of this parasite and its relationship to Irritable Bowel Syndrome have been carried out. To evaluate the in vitro effect of metronidazole and the compound 1,3bis-(4-phenyl-[1,2,3] triazole-1-il)2-propanol over Blastocystis, as well as the prevalence of Blastocystis in patients with Irritable Bowel Syndrome. A prospective, transversal design study (April 2016-April 2017) of 51 samples of patients with Irritable Bowel Syndrome, obtained from the ISSEMyM Medical Center in Toluca, Mexico. For the identification of Blastocystis was done in three serial stool samples through direct microscopic examination and the Ritchie technique. The in vitro susceptibility test towards metronidazole and the triazolic compound was done through a microculture in concentrations of 1 to 1000 lg/ mL, each one in triplicate. A 31.3% prevalence of Blastocystis was observed in the population, with greater prevalence in women (30.2%) than in men (25%). In the susceptibility test, a CL 50 of 64 lg/mL was obtained for metronidazole, in comparison to the CL 50 of 250 lg/mL for 1,3-bis-(4-phenyl-[1,2,3] triazole-1-il)2-propanol. This molecule in development has an effect for the treatment of infection by Blastocystis in vitro in patients with IBS and therefore, more studies should be performed.

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Observações sobre Blastocystis hominis e Cyclospora cayetanensis em exames parasitológicos efetuados rotineiramente

Abstract: RESUMO Relatamos algumas observações, efetuadas com exames parasitológicos de fezes, em atividades rotineiras: os métodos de

Cited by 8 publications

References 12 publications

Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites

Concordance between the zinc sulphate flotation and centrifugal sedimentation methods for the diagnosis of intestinal parasites

Padronização do diagnóstico de Blastocystis hominis por diferentes técnicas de coloração

Evaluation of the effect of 1,3-bis-(4-phenyl-[1,2,3] triazole-1-il)2-propanol in comparison with metronidazole in an in vitro culture of Blastocystis in samples of patients with irritable bowel syndrome

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