Influência dos diferentes períodos de colheita na expressão de isoenzimas em sementes de cevada

Tunes, Lilian Vanussa Madruga de; Badinelli, Pablo Gerzson; Barros, Antônio Carlos Souza Albuquerque; Meneghello, Géri Eduardo; Amarante, Luciano do

doi:10.1590/s0034-737x2011000200008

Cited by 12 publications

(10 citation statements)

References 14 publications

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“…This enzyme operates in the alternative pentose phosphate pathway and is responsible for maintaining an adequate level of NADPH in cells, and malate dehydrogenase, which catalyzes the conversion of malate to oxaloacetate, having an important role in the Krebs cycle. In addition, glucose-6-phosphate dehydrogenase, participates in malate movement through the mitochondrial membrane in other cellular compartments (Tunes et al, 2011). In wheat seedlings that low concentration of glucose enhance the activity of antioxidant enzymes, such as peroxidase, catalase and superoxide dismutase (Hu et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Biochemical profiling and its role in physiological quality of maize seeds

2018

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-Viability and vigor of seeds are physiological attributes influenced by a myriad of factors including the genotype. Biochemical profiling of maize seeds and its correlation with their physiological quality is of crucial importance in breeding programs that the aim is seeds of better quality. The main goal of this study was to analyze the genetic diversity among maize inbred lines and evaluate the biochemical components that have an important role in physiological quality of seeds. The seeds of the inbred lines and hybrids were submitted to viability test, vigor by the accelerated aging and cold tolerance, and biochemical profiling (total protein content, soluble proteins, total phosphorus, phytate, inorganic phosphorus, starch and soluble sugars). The genetic divergence between the inbred lines found, prompt us to conclude that genetic gains may exist in association with physiological quality of maize hybrids. Seeds with higher inorganic phosphorus and soluble sugars presented superior physiological quality than seeds with lower levels of those compounds.Index terms: vigor, viability, genetic divergence, stored reserves, Zea mays.Perfil bioquímico e seu papel na qualidade fisiológica de sementes de milho RESUMO -A viabilidade e o vigor de sementes são atributos fisiológicos influenciados por uma série de fatores, incluindo o genótipo. O perfil bioquímico das sementes de milho e sua correlação com a qualidade fisiológica são importantes para programas de melhoamento que objetivam obter sementes de melhor qualidade. O objetivo deste estudo foi analisar a diversidade genética entre as linhagens de milho e avaliar os componentes bioquímicos que têm um papel importante na qualidade fisiológica das sementes. As sementes das linhagens e dos híbridos foram submetidas ao teste de viabilidade, vigor pelo envelhecimento acelerado e tolerância ao frio e a determinação do perfil bioquímico (teor de proteína total, proteínas solúveis, fósforo total, fitato, fósforo inorgânico, amido e açúcares solúveis). A divergência genética encontrada entre as linhagens permite concluir que os ganhos genéticos podem existir em associação com a qualidade fisiológica dos híbridos de milho. As sementes com maior teor de fósforo inorgânico e açúcares solúveis apresentaram qualidade fisiológica superior à das sementes com níveis menores desses compostos.Termos para indexação: vigor, viabilidade, divergência genética, reservas armazenadas, Zea mays.

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Section: Resultsmentioning

confidence: 99%

Biochemical profiling and its role in physiological quality of maize seeds

2018

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“…Environmental stresses such as those caused by exposure of plants and seeds to cold temperature can also result in changes in the expression of Root (cm) enzymes (Mertz et al, 2009). Isoenzymes are products of gene expression and consequently highly infl uenced by the environment (Tunes et al, 2011). Low ambient temperatures caused changes in enzyme patterns, especially catalase ( Figure 3A).…”

Section: Resultsmentioning

confidence: 99%

Physiological, isozyme changes and image analysis of popcorn seeds submitted to low temperatures

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2017

J. Seed Sci.

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-This present study aimed to assess seed quality of popcorn seedlings subjected to low temperatures by examining their physiological changes, enzymes, and images. We used a completely randomized design with four replications in a factorial arrangement with four hybrids (P618, AP6002, AP8202, AP8203) and five temperatures (10, 13, 16, 19 and 25 °C). Their physiological quality was assessed through radicle protrusion, germination, dry mass of seedlings and emergence. We have assessed the images of seedlings through their hypocotyl and root lengths; and through rates of automatic force, uniformity and growth, provided by Groundeye. The isozyme expressions were determined for the catalase (CAT -IUBMB: EC 1.11.1.6), alcohol dehydrogenase (ADH -IUBMB: EC 1.1.1.1), malate dehydrogenase (MDH -IUBMB: EC: 1.1.1.37) and α-amylase (α-AMY -IUBMB: EC 3.2.1.1). Results show that low temperatures cause a negative effect on seed physiological quality in enzyme expression of CAT, ADH, MDH and α-AMY and on the performance of seedlings. These alterations compromise seed quality. Hybrids AP6002 and AP8203 should be recommended for sowing at up to 16 °C. The Groundeye software was efficient in the analysis of popcorn seedlings and in for the assessment of seed quality when submitted to low temperatures.

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“…The highest activity of this enzyme occurs at temperatures between 20°C and 30°C, which could be related to synthesis of amino acids on the metabolic pathway during germination. Tunes et al (2011) worked with two barley cultivars harvested in three seasons, and observed that seedlings showed no activity in cultivars harvested at 118 days after sowing (DAS). When harvest was carried out at 129 DAS, the enzyme was present only in 'Scarlett' cultivar.…”

Section: Figure 1 Profile Of the Esterase (Est) Enzyme Using Lettucementioning

confidence: 99%

Germination temperatures affect the physiological quality of seeds of lettuce cultivars

et al. 2019

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The objective of this study was to evaluate the physiological quality allied to biochemical quality of lettuce seeds by germination and enzymes expression at 20, 25, 30, 35, 40 and 42ºC. Germination speed index and percentage of germination were estimated. Isoenzyme expressions were assessed by alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), catalase (CAT), esterase (EST), pyruvate decarboxylase (PDC) and glutamate oxaloacetate transferase (GOT). The experiment consisted of a completely randomized design in a factorial scheme 4x6, with four cultivars and six different temperatures, with four replications. The highest germination and vigor were observed for cv. 'Everglades' at 35°C, which proved that this cultivar is thermotolerant. Catalase can be considered a genetic marker for the identification of thermotolerant lettuce cultivars. Cultivar 'Everglades' has potential to be used in lettuce breeding programs aimed at cultivars tolerant to high temperatures during germination.

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Influência dos diferentes períodos de colheita na expressão de isoenzimas em sementes de cevada

Cited by 12 publications

References 14 publications

Biochemical profiling and its role in physiological quality of maize seeds

Biochemical profiling and its role in physiological quality of maize seeds

Physiological, isozyme changes and image analysis of popcorn seeds submitted to low temperatures

Germination temperatures affect the physiological quality of seeds of lettuce cultivars

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