BACKGROUND: Nursing professionals that work in Emergency and Urgency services, daily carry out direct patient care, in a high stimuli situation and permanent work pressure; therefore it is important to develop a questionnaire that helps establishing an adequate scientific, technical and ethical profile, for nurses who work in Emergency and Urgency hospital areas. METHODS: The aim of this study was to validate a questionnaire able to identify the profile that Emergency and Urgency Services nurses should have. The questionnaire consisted of 32 items, and was validated by 4 experts; Fleiss Kappa coefficient was used to evaluate the agreement between judges. RESULTS: One of the items ( question No.12) was inadequate for all of the judges and had to be withdrawn; 5 items (questions 1,5,9,11 and 19) needed changes, so in accordance with the judges requirements, these changes were made; a total of 26 items obtained an “almost perfect” concordance of totally adequate items. CONCLUSION: The original questionnaire ended with a total of 31 items and has the required content validity. This questionnaire can be validated in the construct through the use of a pilot test applied to nurses in Emergency and Urgency services. This questionnaire makes it possible to evaluate the profile of the nursing staff prior to working in the emergency area. http://dx.doi.org/ KEYWORDS: Emergency nursing, expert testimony, validation study