Técnicas moleculares para caracterização e conservação de plantas medicinais e aromáticas: uma revisão

Souza, Damázio Campos de

doi:10.1590/1983-084x/13_071

Cited by 20 publications

(17 citation statements)

References 55 publications

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“…Similarly, genetic divergence can be evaluated by means of molecular markers, enabling germplasm characterization (Souza, Carvalho, Martins, Guedes, & Oliveira, 2008), phylogenetic analysis, DNA fingerprinting, gene link detection with mono and polygenic characters, identification of varieties, gene introgression, and indirect selection of agronomic traits. The effectiveness of different types of labels is confirmed by the wide use of these techniques in in genetic storage studies and for improving plants (Souza, 2015).…”

Section: Introductionmentioning

confidence: 94%

<b>Genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors

et al. 2017

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ABSTRACT. This study aimed to evaluate the genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors. The experiment was conducted in the Olericulture Sector at Federal University of Jequitinhonha and Mucuri Valleys (UFVJM) and evaluated 60 sweet potato genotypes. For morphological characterization, 24 descriptors were used. For molecular characterization, 11 microsatellite primers specific for sweet potatoes were used, obtaining 210 polymorphic bands. Morphological and molecular diversity was obtained by dissimilarity matrices based on the coefficient of simple matching and the Jaccard index for morphological and molecular data, respectively. From these matrices, dendrograms were built. There is a large amount of genetic variability among sweet potato genotypes of the germplasm bank at UFVJM based on morphological and molecular characterizations. There was no duplicate suspicion or strong association between morphological and molecular analyses. Divergent accessions have been identified by molecular and morphological analyses, which can be used as parents in breeding programmes to produce progenies with high genetic variability. Dissimilaridade genética entre genótipos de batata-doce por meio de descritores morfológicos e moleculares RESUMO. Objetivou-se avaliar a dissimilaridade genética entre genótipos de batata-doce por meio de descritores morfológicas e moleculares. O experimento foi conduzido no Setor de Olericultura da Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) avaliando-se 60 genótipos de batata-doce. Na caracterização morfológica, utilizaram-se 24 descritores. Na caracterização molecular, utilizaram-se onze primers microssatélites específicos para batata-doce, obtendo-se 210 bandas polimórficas. A diversidade morfológica e molecular foi obtida por matrizes de dissimilaridade baseando-se no coeficiente de coincidência simples e índice de Jaccard, para dados morfológicos e moleculares, respectivamente. A partir destas matrizes foram construídos dendrogramas. Houve grande variabilidade genética entre os genótipos de batata doce do banco de germoplasma da UFVJM, tanto pela caracterização molecular como morfológica. Não houve suspeita de duplicata nem associação entre a análise molécular e morfológica. Foram identificados acessos divergentes pelas análises moleculares e morfológicas, os quais são indicados como genitores para compor programas de melhoramento a fim de obter progênies com alta variabilidade genética. Palavras-chave:Ipomoea batatas (L.) Lam., marcadores morfológicos, microssatélites, diversidade genética, banco de germoplasma.

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Section: Introductionmentioning

confidence: 94%

<b>Genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors

et al. 2017

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“…morphological, agronomic, and molecular characterization. Because inter-and intraspecific variability is detected in molecular characterization via markers (Souza, 2015;Pereira et al, 2016), it is an effective method in the study of genomes, as polymorphism can be identified directly at the DNA level without environmental influences (Ferreira and Grattapaglia, 1998). Among the markers based on PCR amplification are microsatellites, also known as SSR (Simple Sequence Repeat) markers.…”

Section: Introductionmentioning

confidence: 99%

Research Article Genetic diversity of cassava landraces cultivated in northern Mato Grosso State, Brazil, using microsatellite markers

Pedri¹,

Hoogerheide²,

Tiago³

et al. 2019

Genet. Mol. Res.

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Cassava (Manihot esculenta) is a traditional crop in tropical and subtropical regions that is used for human consumption and in animal feed. This studied cassava landraces cultivated in northern Mato Grosso State, Brazil, to detect the variability in family farms, aiming at the preservation and use potential of these genetic resources. A total of 120 M. esculenta individuals were evaluated at the rate of 40 per location (population), where each landrace was represented by 10 plants. The 14 microsatellite markers examined showed genetic diversity. The average polymorphic information content (PIC) was 0.621 and the average number of alleles per locus was seven. Genetic diversity parameters indicated that H o was higher than H e for the three populations, showing negative fixation indices and a lack of inbreeding. AMOVA revealed greater molecular variation within the populations (92%). Bayesian analysis and the ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 18 (3): gmr18315 E.C.M. Pedri et al. 2UPGMA clustering method resulted in two main groups formed with the individuals distributed randomly; i.e., regardless of collection site (location). The gene flow found in this study is a consequence of the introduction and exchange of genetic material (landraces) performed by the farmers, who act on their farms as maintainers of local diversity. Manihot esculenta cultivation in northern Mato Grosso State ensure the on-farm conservation of genetic variability of the species and constitutes a source of genetic resources such as genes of resistance and adaptation, which can be exploited and utilized in breeding programs.

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“…The economic potential of medicinal and aromatic plants has contributed to the significant growth of the interest for germplasm characterization and conservation (Souza, 2015). Germplasm characterization allows the insertion of materials of interest in breeding programs and serves as a basis for the design of conservation strategies.…”

Section: Introductionmentioning

confidence: 99%

“…Several molecular markers are available in the market, and the knowledge on the properties and applications of the species is necessary to choose the appropriate marker according to the profile of the study of interest (Souza, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Brazil has about 300 Croton species in different types of vegetation. Croton tetradenius Baill., which is endemic to the Northeast region and predominant in the Caatinga vegetation, stands out among the several species of this genus. Considering the importance of knowing the genetic variability of a species, the objective of this study was to analyze the genetic diversity of the genotypes of natural populations of C. tetradenius in the State of Sergipe, using ISSR molecular markers. Forty individuals were sampled in four natural populations of the State of Sergipe, Brazil. Thirteen primers were used for DNA amplification using ISSR-PCR, totaling 77 amplified fragments, of which 94.8% were polymorphic. Results of the cluster analysis obtained by the Jaccard's similarity index, using the UPGMA method, resulted in the formation of six distinct clusters. Analysis of molecular variance (AMOVA), used to estimate the genetic variability among populations, revealed significant genetic variance (P < 0.01) between and within the 2 C.S. Almeida-Pereira et al.Genetics and Molecular Research 16 (2): gmr16029602 studied populations, and most of the genetic diversity was found (87%) within populations. According to the Jaccard's similarity index, none of the studied plants was genetically identical. CTE210 and CTE305 presented high similarity index (0.76), while CTE105 presented low similarity index (<0.16) with all related individuals. ISSR markers were efficient and allowed the formation of a molecular profile, and had sufficient polymorphism to estimate the genetic variability between the accessions of the studied populations.

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Técnicas moleculares para caracterização e conservação de plantas medicinais e aromáticas: uma revisão

Cited by 20 publications

References 55 publications

<b>Genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors

<b>Genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors

Research Article Genetic diversity of cassava landraces cultivated in northern Mato Grosso State, Brazil, using microsatellite markers

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

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