Fibroblast and pre-osteoblast cell adhesive behavior on titanium alloy coated with diamond film

Abstract

It is well known that titanium alloys have mechanical strengths comparable to steels, as well as high corrosion resistance. Also, they have the advantage of promoting osseointegration, when used in medical and dental implants. This work aims to describe the adhesion properties of fibroblast and osteoblast cells on the surface of titanium aluminum vanadium alloy (Ti6Al4V). Three different conditions of the surface were investigated: smooth, rough and covered with diamond film. Conventional material characterizations were performed to the film which consisted in: Morphological visualization by scanning electron microscopy, confocal profilometry, X-ray diffraction pattern, Raman backscattering spectroscopy and atomic force microscopy. Biocompatibility tests of Ti6Al4V were performed using primary human fibroblasts and mouse pre-osteoblasts cell line MC3T3-E1. Overall, diamond films deposited on Ti6Al4V showed interesting results of uniformity and protection against cracks on to the surface, reasonable biocompatibility features if compared to uncovered ones, indicating that this film is an alternative for using in health care applications.

Physicochemical and Biological Evaluation of Chitosan-Coated Magnesium-Doped Hydroxyapatite Composite Layers Obtained by Vacuum Deposition

“…The 2D topographies of the fibroblast cells adhered to the surface of unirradiated MgHApCh composite thin films after 24 h of incubation and their 3D representation are depicted in Figure 12a,b. The adherence and proliferation of the fibroblast cells on the surface of the MgHApCh composite thin films irradiated with a dose of 3 Gy of gamma radiation are depicted in the 2D AFM topography and its 3D representation from Figure 12c,d The AFM 2D topographies suggested that on the surface of the MgHApCh composite thin films could be found the typical patterns of the cellular morphology of fibroblast cells presenting morphologies of flattened cells varying from spindle-like to tile-like [62][63][64][65][66][67]. In addition, both the 2D AFM topographies and their 3D representations highlighted that after an incubation period of 24 h, the fibroblast cells had a very good adherence to the studied MgHApCh composite thin film surfaces.…”

“…The fibroblast cells measure several hundreds of micrometers, which exceed the normal range of an AFM scan. For this reason, the topogra- The AFM 2D topographies suggested that on the surface of the MgHApCh composite thin films could be found the typical patterns of the cellular morphology of fibroblast cells presenting morphologies of flattened cells varying from spindle-like to tile-like [62][63][64][65][66][67]. In addition, both the 2D AFM topographies and their 3D representations highlighted that after an incubation period of 24 h, the fibroblast cells had a very good adherence to the studied MgHApCh composite thin film surfaces.…”

Human Embryonic Stem Cell-Derived Mesenchymal Progenitor (hESCs-MP) Growth on Nanostructured Ti6Al4V Surfaces

“…Considering the results obtained in the present work, it is possible to evidence that the increase in nanometric roughness had a more significant effect than wettability on cell growth in osteogenic media. However, cell attachment and growth are complex processes that involves several surface features, including topographic morphology, surface roughness, surface chemistry, and phase state, extending well beyond the realm of surface nanostructures 47,64 .…”