Experimental furcal perforation treated with mineral trioxide aggregate plus selenium: immune response

Abstract

The aim of this study is to evaluate the expression of cytokines in response to mineral trioxide aggregate (MTA) plus selenium in germ-free mice with experimental furcal perforation. The first left maxillary molar was opened, and the furcal area was perforated and treated with post-MTA-Se (experimental group). The same surgical intervention was performed for the maxillary right first molar, which was treated with MTA (control group). Fifteen mice were sacrificed 7, 14, and 21 days after furcal perforation, and periapical tissue samples were collected. The mRNA expression levels of the cytokines TGF-β, TNF-α, IFN-γ, HPRT, IL-10, IL-4, RANK, RANKL, IL-1, and IL-17 were assessed by using real-time polymerase chain reaction. In the experimental group, at 21-days post-MTA-Se sealing, the mRNA levels of TNF-α and IL-10 were upregulated compared with those in the control group (p < 0.05). Futher assessment revealed basal mRNA expression levels of IL-1α, IFN-γ, RANK, RANKL, IL-17A, IL-4, and TGF-β, over long experimental times, in both the experimental and control groups (p > 0.05). In conclusion, MTA+Se sealing favoured increased expression of IL-10 and TNF-α at later time points (day 21).

Cytotoxicity, cytoprotection and morphological analysis of MTA, MTA Repair HP and Biodentine

“…In this study, the statistical analysis of the MTT test data did not show any differences between the three materials in 24 hours. The results obtained in this study are consistent with those obtained previously (Duarte et al, 2018;Espaladori et al, 2018), that showed non-cytotoxic effect of the MTA. The tested materials herein demonstrated a significant cytoprotection effect against the damage caused by hydrogen peroxide.…”

MTA In The Treatment Of Furcation Perforation In Upper Molars Under Operative Microscopy: Case Report

“…Studies evaluating the immune response induced by MTA have observed that this material is able to stimulate a protective host inflammatory response, positively regulating the expression of proinflammatory cytokines in the early phase after its application (7 to 14 days), while favoring the expression of immunoregulatory cytokines in a late phase (21 days), which would be able to lead to tissue repair (Lara et al, 2015;Espaladori et al, 2018).…”