Cytotoxicity, cytoprotection and morphological analysis of MTA, MTA Repair HP and Biodentine

Abstract

The aim of the present study was to evaluate the in vitro cytotoxicity, cytoprotection and morphological changes by SEM technique of MTA, MTA-HP and Biodentine, on fibroblast 3T3 cell line. MTA, MTA-HP, and Biodentine were disposed into teflon sterile discs and incubated in culture media for 24 hours to obtain elutes. Fibroblast 3T3 cells were cultured with the respective elutes and control group with culture medium. Cytotoxicity and cytoprotection assays were determined by MTT method. The results were statistically processed by Mann-Whitney (α=0.05) and Kruskal-Wallis analysis. Cells cultured in coverslips and treated with the elutes were submitted to fixation and dehydration process to evaluate morphological alterations by SEM technique. In cytotoxicity assay, cells treated with MTA, MTA HP and Biodentine showed viability above 95%, like control cells. In cytoprotection to the 3T3 cells, materials promoted at the same magnitude (p>0.05), with improved cell growth and were considered statistically different from the obtained for cells only treated with peroxide solution (positive control) (p=0.046). Also, viability results of the tested root canal materials were close to that of the negative control (cells treated only with culture medium) (p=0.05). No morphologic cell changes of 3T3 cells in contact with the endodontic materials were revealed by SEM technique. The bioceramic materials has demonstrated high bioactivity and biocompatibility, as presented in cytoprotection and morphological trials.

Use of Biodentine in the resolution of a failure in paraendodontic surgery

“…Several reactions occur at the interface between cells and the biomaterial (Tomás-Catalá et al, 2018). Among these reactions, there is the bioactivity where there is an interaction and a stimulus to the tissue in contact with the biomaterial (Michelotto et al, 2022). Therefore, the objective of the case report below was to show the rapid bone repair obtained with the use of Biodentine, as a filling material in the mesial canals of a maxillary molar, with symptomatic apical periodontitis, which had already undergone endodontic treatment and paraendodontic surgery.…”