Influence of diabetes mellitus on the mineralization ability of two endodontic materials

Abstract

The aim of this study was to evaluate the influence of diabetes mellitus on tissue response and mineralization ability of Sealapex ® and MTA Fillapex ® sealers. Twenty-four Wistar rats were divided into two groups: diabetic and non-diabetic. The materials were placed in polyethylene tubes and implanted into dorsal connective tissue of rats for 7 and 30 days. Six animals from each group received injection of calcein, alizarin, and oxytetracycline on days 7, 14, and 21, respectively. The animals were killed after 7 and 30 days and specimens were prepared for histologic analysis by staining with hematoxylin and eosin or Von Kossa or left unstained for polarized light or fluorescence microscopy. On day 7, inflammatory reactions were characterized. Moderate inflammatory responses were observed for all groups and on day 30, a mild inflammatory response against MTA Fillapex ® and a moderate inflammatory response against Sealapex ® were observed. Von Kossa-positive structures were observed in response to both materials and birefringent structures were observed upon polarized light analysis; these had no relation to the diabetic condition (p > 0.05). The fluorescence intensity was unaffected in diabetic rats (p > 0.05).In conclusion, diabetes mellitus did not influence the tissue response or mineralization stimulated by Sealapex ® or MTA Fillapex ® .

Cytotoxicity and biocompatibility of a new bioceramic endodontic sealer containing calcium hydroxide

“…3,8 The MTA Fillapex (Angelus Indústria de Produtos Odotontológicos S/A, Londrina, Brazil) is an endodontic sealer that contains MTA, radiopacifiers, and resins in its composition. 9 Previous studies exhibited that the sealer exhibited flowability, dimensional change, radiopacity, and solubility, and this is in agreement with International Organization for Standardization 6876 (ISO 6876/2001). 10,11 Additionally, it exhibited an adequate setting time that is suitable for an endodontic sealer.…”

“…4,22 In our histological analysis, a reduction in the inflammation caused by MTA Fillapex was observed over time despite increase in inflammation observed in relation to the Control group, thereby suggesting that it is more biocompatible with increases in the analysis times as reported in extant studies. 9 The same occurred with AH Plus given that the histological results indicate that the inflammation generated by the sealer decreases over time and can be better evaluated over longer periods of analysis. Other previous studies (both in vitro or in vivo) indicated that AH Plus is considered as biocompatible by allowing reduction of inflammation and cytotoxicity over time.…”

“…10,11 Additionally, it exhibited an adequate setting time that is suitable for an endodontic sealer. 12,13 However, a consensus with respect to the biocompatibility and cytotoxicity of the material is absent since extant studies indicate both favourable 9 and unfavorable results in terms of their use. 14,15 Thus, new bioceramic materials appear in the market in order to obtain an ideal endodontic sealer.…”

Hyperglycemic condition influence on mineral trioxide aggregate biocompatibility and biomineralization

“…Thus, to better understanding the influence of hyperglycemia on tissue response, mineralization ability and cytokine production of both MTAs, a subcutaneous implantation study was performed. Additionally, to induce hyperglycemia in animals we used Alloxan (Gomes Filho et al, ; Gomes‐Filho et al, ). Alloxan and Streptozotocin are the most utilized chemicals drugs to induce hyperglycemia in experimental animals and while some researches reporting that Alloxan is hydrophilic compound similar to glucose, chemically unstable with short half‐life (Lenzen, ; Lenzen & Munday, ) and due the greater stability Streptozotocin is the agent of choice to this induction, these both drugs are widely used and recommended and also exhibit cytotoxic action in β cells of the pancreas (Lenzen, ; Szkudelski, ).…”

Endodontic medicine: interrelationships among apical periodontitis, systemic disorders, and tissue responses of dental materials

“…In addition, no evidence of the direct correlation among diabetes mellitus and inflammatory response or mineralization ability for Sealapex and MTA Fillapex sealers was detected in a similar investigation. 94 Furthermore, the local and systemic effects of both Gray and White MTA on bone marker expression after subcutaneous implantation in diabetic rats were investigated by Queiroz et al 80 Immunohistochemical analysis of osteocalcin and osteopontin production and biochemical analyses of calcium, phosphorus, and serum alkaline phosphatase levels at 7 and 30 days were performed. At day 7, calcium and phosphorus levels were higher in the GMTA group than in the WMTA group.…”