Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods

Duffeck, Maíra Rodrigues; Tibola, Casiane Salete; Guarienti, E. M.; Ponte, Emerson M. Del

doi:10.1590/1678-992x-2016-0263

Cited by 19 publications

(14 citation statements)

References 25 publications

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“…The mean concentration reported for DON (240 µg/kg) [14] was similar to the level described in the present study. In a recent survey conducted in the Brazilian states of Sã o Paulo, Paraná and Rio Grande do Sul Brazil [15], ZEA and DON were found in 39% and 55% of wheat grain samples, respectively, with overall mean levels of 79.78 µg/kg and 795.2 µg/kg, respectively, which is much high than the values reported in our work. In the present study, DON was also detected in one sample (8.3%) of bean, as shown in Table III.…”

Section: Resultscontrasting

confidence: 79%

Performance and Application of a “Dilute-and-Shoot” LC-MS/MS Method for Determination of Mycotoxins in Food Products in São Paulo, Brazil

Franco¹,

Petta²,

Vendrametto³

et al. 2018

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Mycotoxins are secondary metabolites produced by several species of fungi that occurs in agricultural commodities, which cause a great variety of toxic effects in vertebrates, including humans. In this study, a "dilute-andshoot" method using ultra-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was evaluated and applied to assess the incidence of aflatoxins (AF) B 1 , B 2 , G 1 and G 2 , fumonisins (FB) B 1 and B 2 , ochratoxin A (OTA), zearalenone (ZEA) and deoxynivalenol (DON) in samples of corn meal (N = 4), corn flour (N=2), wheat flour (N=4), rice (N=14) and bean (N=12) collected in small-scale farms from São Paulo, Brazil. The performance parameters (aparent recovery, matrix effect, extraction recovery, linearity, limit of quantification and limit of detection) were satisfactory, and the most frequent mycotoxin detected in food samples was FB (7.11-316.04 g/kg) below the Brazilian regulations. DON was detected in all samples of wheat flour (162.49-324.66 g/kg) along with FB in 3 samples, and in one sample of bean (46.52 g/kg). ZEA was detected in 2 samples of rice (4.90-6.78 g/kg). Results indicated low incidences of mycotoxin in the products evaluated, although the co-occurrence of FB and DON warrants concern about their incidence in wheat flour in Brazil.

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Section: Resultscontrasting

confidence: 79%

Performance and Application of a “Dilute-and-Shoot” LC-MS/MS Method for Determination of Mycotoxins in Food Products in São Paulo, Brazil

Franco¹,

Petta²,

Vendrametto³

et al. 2018

ijfe

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“…The method was approved by the AOAC Research Institute (Certificate Nº 110,701). The accuracy, sensitivity, and specificity of the ELISA method compared with a chromatographic method, for mycotoxin analysis, were determined in a previous study (Duffeck, Tibola, Guarienti, & Del Ponte, 2017).…”

Section: Deoxynivalenol Analysismentioning

confidence: 99%

Effect of debranning process on deoxynivalenol content in whole‐wheat flours

et al. 2019

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Background and objectives Post‐harvest mitigation strategies are essential to reduce mycotoxin contamination in wheat‐based foods. The study aimed to evaluate the effect of the debranning process on the deoxynivalenol content in whole‐wheat flour naturally contaminated by Fusarium spp. Thirty commercial wheat samples were used and were obtained in Southern Brazil Region in 2015 crop season. Findings The highest DON contamination level was found in the no‐debranned samples and the outermost fraction (extraction rate >95%), which corresponds to the debranning time of 15 s. When all debranning times were included in the analysis, only the samples from Paraná State had the DON content significantly reduced after the debranning. Conclusions The debranning process reduced the DON content in whole‐wheat flour with lower to moderate levels of contamination, corresponding to DON content of 1.032 and 1.174 µg/kg, obtained in Paraná and Rio Grande do Sul States, respectively. Significance and novelty These results are important for wheat supply chain to meet the legislation requirements and to produce safer foods.

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“…The mean concentration reported for DON (240 µg/kg)(LIU et al, 2015) was similar to the level described in the present study. In a recent survey conducted in the Brazilian states of São Paulo, Paraná and Rio Grande do Sul Brazil(DUFFECK et al, 2017), ZEN and DON were found in 39% and 55% of wheat grain samples, respectively, with overall mean levels of 79.78 µg/kg and 795.2 µg/kg, respectively, which is much high than the values reported in our work. In the present study, DON was also detected in one sample (8.3%) of bean, as shown inTable 8.…”

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confidence: 80%

Exposure assessment to multiple mycotoxins in rural areas of São Paulo and Santa Catarina states, Brazil

Franco¹

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Gostaria de primeiramente agradecer a Deus por permitir que esta pesquisa fosse possível e por me dar força e coragem para lidar com todos os desafios. Agradecer ao meu pai e minha mãe, por sempre me apoiarem com as minhas escolhas, por apoiarem o caminho que escolhi para seguir em minha vida e por me darem força e suporte durante o período do meu doutorado. E a minha irmã Beatriz pela paciência e por me ajudar quando estive longe. Obrigada família! Gostaria de agradecer ao professor Carlos por sempre acreditar em mim e no meu potencial, por todo apoio e suporte oferecido, e por toda paciência com minha falta de experiências. Obrigada professor Carlos por sua orientação, pelas trocas valiosas de conhecimentos e pela amizade. I thank George Rottinghaus for everything. Thank you for the opportunities you gave to me. Thank you for the friendship. And Thank you for teaching me so much. A Tânia Petta por todo suporte, ensinamentos e ajuda dada. Obrigada por ser paciente e estar sempre disponível para me ajudar e ensinar, sem você este projeto não teria caminhado. Ao professor Corassin e a Roice, pela ajuda, paciência e empenho em solucionar os diversos problemas que surgiram em todos esses anos. Agradeço ao Everton Angelotti que me acompanhou em grande parte desta jornada, sempre com muito carinho, me aconselhando e me apoiando nas minhas decisões. Aos estagiários que foram essenciais para a realização da pesquisa especialmente Ilca Pasquotto, Paula Maniglia, Maria Eugênia Vendrametto, Gabriela Zambelli e Eduarda Georges. Por todo esforço e dedicação, muito obrigada por tudo. Muito obrigada Amanda Cirelli, pela amizade e companheirismo, paciência nos momentos dos meus surtos e por toda ajuda dada durante minha pesquisa. Ao Romulo Uliana, por sempre estar disposto a me ajudar. Vocês fizeram a diferença no meu doutorado. Obrigada Keliani por toda ajuda. Obrigada por me emprestar seus pais e sua casa, e por me darem todo suporte em Erval Velho-SC. Obrigada ao professor Gilmar pela ajuda dada em Pinhalzinho-SC. Obrigada ao funcionário Diógenes, pela ajuda nas propriedades de Pirassununga-SP e a Patricia que permitiu o estudo em sua granja em Descalvado-SP. Obrigada a república Havaianas por me acolher e pelas amizades criadas. Aos amigos portugueses, Paula Alvito e Ricardo Assunção, pela oportunidade de aprendizado que obtive no meu tempo junto a vocês no Instituto Ricardo Jorge. Obrigada pela paciência e por compartilharem seus conhecimentos comigo. Foi uma experiência fantástica, com muito crescimento profissional e pessoal. À CAPES e CPNPq, pelos recursos destinados à execução do projeto e bolsas concedidas. Meus sinceros agradecimentos também a todos os integrantes da Faculdade de Zootecnia e Engenharia de Alimentos, especialmente os funcionários membros da pós graduação e professores. A todos aqueles que, embora não citados, me acompanharam nesta etapa e de alguma forma contribuíram para que estes quase 4 anos fossem mais felizes, o meu reconhecido e carinhoso muito obrigada! "Aos outros, dou o direito de ser como sã...

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Survey of mycotoxins in Southern Brazilian wheat and evaluation of immunoassay methods

Cited by 19 publications

References 25 publications

Performance and Application of a “Dilute-and-Shoot” LC-MS/MS Method for Determination of Mycotoxins in Food Products in São Paulo, Brazil

Performance and Application of a “Dilute-and-Shoot” LC-MS/MS Method for Determination of Mycotoxins in Food Products in São Paulo, Brazil

Effect of debranning process on deoxynivalenol content in whole‐wheat flours

Exposure assessment to multiple mycotoxins in rural areas of São Paulo and Santa Catarina states, Brazil

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