Evaluation of Petrifilm™ system compared with traditional methodology in count of indicators of sanitary-hygienic quality and pathogenic microorganisms in sheep milk

Abstract

The objective of this study was to evaluate the applicability of the Petrifilm™ plates to enumerate microbial groups in sheep milk. Samples of sheep milk (n = 30) were plated simultaneously, to enumerate mesophilic aerobes, total coliforms, lactic acid bacteria, Staphylococcus aureus and Escherichia coli, using convencional reference protocols and Petrifilm™ plates. The results were compared using McNemar's test, linear regression and ANDVA (p < 0,05). The results demonstrated good significant between conventional methodologies and Petrifilm™ plates. Further, the Petrifim™ STX for counting S. aureus had higher recoverability of bacteria compared with the conventional methodology. Based on the results obtained and in view of the ease and rapidity procedures results, Petrifim ™ plates may be considered as alternatives for microbiological testing in sheep milk.Keywords: milk analyzes; sheep's milk; Escherichia coli; Aerobic mesophilic bacteria.Practical Application: This study generated knowledge for the practical application of the Petrifilm™ system in microorganisms search in sheep milk. From this study was possible to conclude that the use of this system is reliable for microorganisms surveyed, facilitating the work, and generate faster results, an important factor when it comes to monitoring of food-borne outbreaks. Thus, it was possible to verify the adequacy of Petrifilm™ system as a new alternative for enumeration of mesophilic aerobic microorganisms, coliforms, lactic acid bacteria, Staphylococcus aureus and Escherichia coli. Finally, the use of Petrifilm™ system generated reliable results more easily and more quickly, including facilitating the development of the work in the laboratory microbiological analyzes of food, because of no necessity of using large quantities of materials, glassware, petri plates, pipettes, while minimizing the preparation of material for the analyzes.

Real-Time Monitoring the Effects of Storage Conditions on Volatile Compounds and Quality Indexes of Halal-Certified Kimchi during Distribution Using Electronic Nose

“…Lactic acid bacteria were counted by the 3M Petrifilm lactic acid bacteria count plate method 6461/6492. The evaluation of Petrifilm is a reliable LAB enumeration method compared with the traditional methodology [ 31 , 32 ]. In sterile conditions, portions of Kimchi (10 g) were obtained from each sample and diluted 10-fold with 90 mL of 0.85% saline solution in a sterile filter bag.…”

Isolation of Escherichia coli and Staphylococcus aureusin raw milk from refrigeration tanks: identification and antimicrobial resistance profiles

“…In addition to conventional microbiological analyses, quick analysis methods like Readycult™ -LMX (BELOTI et al, 2002), Rida Count [Coliforms R 1009] (VASALLO; REYES; CAMBAS, 2013), and 3M TM PetrifilmTM Staph (SOUZA et al, 2015) are routinely applied to microbiological milk analyses. Among these methods, 3M TM Petrifilm TM products are recognized and regulated for foods of animal origin by the Brazilian Ministry of Agriculture, Livestock and Supply-MAPA (BRASIL, 2005).…”